{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v02/MSV000083404/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Ondrej Vit"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=d310e8f87dd6406b9ebeacd262aaf955"],"submitter_email":["ondrej.vit@lf1.cuni.cz"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["31"],"ptm_modification":["UNIMOD:11 - \"Homoserine lactone.\"","UNIMOD:10 - \"Homoserine.\"","UNIMOD:7 - \"Deamidation.\"","UNIMOD:1384 - \"Methionine oxidation to homocysteic acid.\"","UNIMOD:893 - \"Carbamidomethylated DTT modification of cysteine.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["Orbitrap Fusion"],"species":["Homo Sapiens (ncbitaxon:9606)"],"submitter_affiliation":["Charles University, First Faculty of Medicine"],"name_synonyms":["Membrane Tissue, membrane, transmembrane, membrane of organ, human being, Man (Taxonomy), Protein., Tissues, Modern, membrane region, Proteins, Tissue, integral to membrane, total expressed protein, Gene, Membrane, membranous organ component, human, Human, Protein Gene Products, Gene Proteins, Membrane Tissues, Homo sapiens, integral component of membrane, Modern Man, Protein, region of membrane, whole membrane, Gene Products, pitchfork, Man, Proteomes, humans"],"description_synonyms":["APEG-1, human being, Procedures, Laundry Detergent Pods, Laundry Pods, Synthetic Detergents, AW125581, Syndet, Germinoblastoma, Malignant, Germinoblastic Sarcoma, Human, Agent, Detergent, Membrane Tissues, Malignant Lymphomas, method, Techniques, Pod, Synthetic Detergent, serial element, Homo sapiens, Method, integral component of membrane, CNM5, Detergents, method used in an experiment, Studies, mKIAA1297, Cell., Lymphomas, Technique, Man, Reticulolymphosarcoma, study, Synthetic, Sarcomas, Man (Taxonomy), Tissues, membrane region, beta-Trypsin, Tissue, Laundry Detergent, Study, Malignant Lymphoma, Sarcoma, Methodological Studies, BPEG, region of membrane, Laundry Pod, pitchfork, D1Bwg1450e, Laundry, Membrane Tissue, membrane, Detergent Pod, Modern, integral to membrane, total expressed protein, segment, Procedure, membranous organ component, Reticulolymphosarcomas, whole membrane, SPEGalpha, Laundry Detergent Pod, Germinoblastic Sarcomas, Cleansing Agents, Germinoblastic, Detergent Pods, Cleansing, transmembrane, membrane of organ, PRSS, Pods, Tripcellim, Glycopeptide, Methodological, MLYM, Membrane, Germinoblastomas, Methodological Study, beta Trypsin, human, plan specification, Trypure, SPEGbeta, lymphoma, Apeg1, Agents, Cleansing Agent, segment - adult, Modern Man, Lymphoma, APEG1, Proteomes, humans, lymphoid cancer"],"citation_count":["0"],"additional_accession":["PXD012599"]},"is_claimable":true,"name":"Three-pronged 'Pitchfork' strategy enables an extensive description of human membrane proteome and identification of missing proteins","description":"In this study, we combined the transmembrane segment-oriented method hpTC with a solid phase enrichment of glycopeptides (SPEG) and standard 'detergent and trypsin' approach into a three-pronged 'Pitchfork' strategy to maximize membrane proteome coverage. The strategy enabled identification of over 1200 IMPs in human lymphoma cells.","dates":{"publication":"Tue Feb 05 05:02:00 GMT 2019"},"accession":"MSV000083404","cross_references":{}}