{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v02/MSV000083839/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Barry Willardson"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=b3567d018a8448acb4b1d6cbb5d0c497"],"submitter_email":["bmwillardson@chem.byu.edu"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["78"],"ptm_modification":["UNIMOD:4 - \"Iodoacetamide derivative.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["LTQ Orbitrap"],"species":["Homo Sapiens (ncbitaxon:9606)"],"submitter_affiliation":["Brigham Young University"],"pubmed_abstract":["The mechanistic target of rapamycin (mTOR) kinase forms two multi-protein signaling complexes, mTORC1 and mTORC2, which are master regulators of cell growth, metabolism, survival and autophagy. Two of the subunits of these complexes are mLST8 and Raptor, β-propeller proteins that stabilize the mTOR kinase and recruit substrates, respectively. Here we report that the eukaryotic chaperonin CCT plays a key role in mTORC assembly and signaling by folding both mLST8 and Raptor. A high resolution (4.0 Å) cryo-EM structure of the human mLST8-CCT intermediate isolated directly from cells shows mLST8 in a near-native state bound to CCT deep within the folding chamber between the two CCT rings, and interacting mainly with the disordered N- and C-termini of specific CCT subunits of both rings. These findings describe a unique function of CCT in mTORC assembly and a distinct binding site in CCT for mLST8, far from those found for similar β-propeller proteins."],"pubmed_title":["Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly."],"pubmed_authors":["Cuéllar Jorge J, Ludlam W Grant WG, Tensmeyer Nicole C NC, Aoba Takuma T, Dhavale Madhura M, Santiago César C, Bueno-Carrasco M Teresa MT, Mann Michael J MJ, Plimpton Rebecca L RL, Makaju Aman A, Franklin Sarah S, Willardson Barry M BM, Valpuesta José M JM"],"description_synonyms":["column., CG1849, d230, POP3, LST8, DAX1, 293tsA1609neo, CMT1E, NROB1, MPP, CMT1A, CMT1B, leg, AHX, dTAFII250, GAS-3, sp110, EfW1, CG1049, extracted material, 293T, l(1)AA33, dmTAF[[II]]230, SRXY2, dmTAF1, Taf230, p63, AI851821, 1, 2, Gable, 0610033N12Rik, mLST8, total congenital with posterior sutural opacities in heterozygotes, TRic, TAF250, AI528772, HEK293T, Taf200, HHG, dTAF[[II]]250, 293-T, TFIID TAF250, cel, cell, CMTDID, Runt, Taf1p, CMT1, CMTDI3, Hek 293T, dTAF250, cataract, HEK-293T, column, l(1)B2/13.1, RUN, Run, Protein GbetaL, total congenital, HMSNIA, TAF, HMSNIB, Tp63, LB5, DAX-1, Human Embryonic Kidney 293T, dTAF[[II]]230, TAF[[II]]250, l(1)LB9, CHM, AA409454, TORC subunit LST8, WAT1, DmelCG1849, TAF200, G protein beta subunit-like, HEK 293 T, l(3)84Ab, hmsnia, BG:DS00004.13, TAFII-250, Rnt, TAF250/230, Cell, AA33, lts8, dTAF230, lLB5, Cct1, CCT1, DSS, TAFII250, HNPP, DmelCG1049, p230, TAF[[II]]250/230, TFIID, CT41283, cct1, Sp110, hnpp, Mammalian lethal with SEC13 protein 8, cmt1e, cmt1a, c-cpn, Taf[[II]]250, dss, TAF[[II]]230, GbetaL, Gbl, GBL, AHCH, CMT2I, CMT2J, TAF[II]250, l(1)19Ea, CG17603, 293 T, TAF[[II]], P0, P235, AI505104, gas-3, DmelCG17603, Taf250, Tcp-1, SR3-5, CMT4E, gbl, Ccta, CCT, Cct, GTD, AHC, HEK-293-T, TAF230, TAF1"],"pubmed_abstract_synonyms":["biochemical pathways, Rap, Forms, Metabolic Process, 2610315D21Rik, POP3, MTORC-2, LST8, ER-Phagy, beta Subunit, FKBP12-rapamycin complex-associated protein, Metabolic Concepts, protein, Progress Reports, l(2)k03905, DmTOR, protein polypeptide chains, Chaperonin Containing TCP1, Cytosolic, p150 target of rapamycin (TOR)-scaffold protein, Roles, Summary Report, symptoms, Concepts, Kinase, RAFT1, Mammalian target of rapamycin, Metabolism Concept, Gable, protein aggregate, Phenomenon, mLST8, Summary Reports, IKKg, total congenital with posterior sutural opacities in heterozygotes, KEY, Key, mTORC1 Complex, TRic, Bird of Prey, mKIAA1303, Man (Taxonomy), Progress Report, catabolism, non-developmental growth of a unicellular organism, rapamycin and nutrient-sensitive TOR complex, proteins, metabolic process resulting in cell growth, dtor, Progress, cataract, Field Reports, ER Phagy, eta Subunit, Nucleophagy, Role Concepts, cellular growth, biotransformation, dRaptor, Chaperonin Containing t Complex Polypeptide, ATP Phosphotransferases, Catabolism, dTOR, dTor, ATP, FK506-binding protein 12-rapamycin complex-associated protein 1, single organism signaling, Tp63, Mechanistic target of rapamycin, screening, dTORC1, Process, metabolism resulting in cell growth, WAT1, Modern, G protein beta subunit-like, Subunit 2, alpha Subunit, Subunit 3, Subunit 1, Ribophagy, Subunit 7, Subunit 4, Subunit 5, Subunit 8, Binding, Cct1, CCT1, DmIKKgamma, Role Concept, Investigative Report, Playthings and Play, Reticulophagy, dIKK, non-developmental cell growth, Kenny, Autophagocytosis, Role, Plaything, Cellular, secretion, cct1, TOR complex 1, TOR complex 2, Prey Bird, RAPT1, c-cpn, TORC 2 complex, Transphosphorylases, death rate, GbetaL, Testis Complex Polypeptide 1, Autophagy, Raptor, nutrient sensitive complex, Rapamycin and FKBP12 target 1, Gbl, GBL, Field, IKK-gamma, Toys, signs, dTOR/dRaptor complex, human, t-Complex Protein 1, 4320, Report, DmelCG16910, 5092, gbl, Ccta, Subunit 6A, Prey Birds, Subunit 6B, humans, zeta1 Subunit, Chaperonin, TORC1, TORC2, 4932417H02Rik, Plays, human being, conformation, Processes, CG5092, Gene, Lipophagy, Metabolic Processes, protein-containing complex, l(2)k17004, CG1049, dIKK-gamma, Human, theta Subunit, Investigative, polypeptide chain, Homo sapiens, Chaperonin-Containing T-Complex Polypeptide 1, Metabolism, p63, DmIKK-gamma, AI851821, Sites, Gene Products, Cellular Autophagy, dRap, CG4320, TOR, dmIKKgamma, 0610033N12Rik, IKK[[gamma]], Metabolism Phenomena, Phosphotransferase, Man, DmelCG8274, delta Subunit, Toy, AI528772, Site, Birds of Prey, Bx34, growth of cell, Playthings, CCT delta Subunit, Complex, Metabolic Concept, flat, tor, Tpr, TPR, Transphosphorylase, t Complex Polypeptide 1, Cytosolic Chaperonin, Combining Site, t-Complex Polypeptide 1, Puppets, IKK, rapamycin and nutrient-insensitive TOR complex, time of survival, raptor/CG4320, Complex 2, Play, Protein GbetaL, site, Torc2, total congenital, Investigative Reports, Frap1, Combining Sites, CT24817, relational structural quality, Chaperonin Containing t-Complex Polypeptide, Puppet, raptor, DmelCG4320, gamma Subunit, TORC 1 complex, findings, degradation, AA409454, protein complex, TORC subunit LST8, Proteins, FRAP1, FRAP2, selective autophagy, function, mTOR, Binding Site, FRAP/TOR, DmelCG5092, Phosphotransferases, Cell, lts8, Concept, Metabolic Phenomena, cell expansion, Metabolism Concepts, IKKgamma, 2.7.11.1, survival, native protein, natural protein, DmelCG1049, Protein, Research Reports, Phenomena, Target of Rapamycin Complex 1, CT41283, Target of Rapamycin Complex 2, 4632407F12Rik, Mammalian lethal with SEC13 protein 8, zeta2 Subunit, Cytosolic Molecular Chaperone CCT, metabolism, mTORC2, mTORC1, Metabolic Phenomenon, Chaperonin Containing TCP 1, multicellular organism metabolic process, Chaperonin CCT, biodegradation, Metabolic, Protein., Kinases, Dmikkgamma, CT24745, Combining, Rapamycin target protein 1, AI327068, FRAP, CG16910, Protein Gene Products, Gene Proteins, AI505104, CT16317, signalling process, Reports, TOR Complex 1, TOR Complex 2, Tcp-1, CG8274, Modern Man, MTOR, epsilon Subunit, CCT, Cct, Summary, Field Report, Chaperonin Containing T Complex Polypeptide 1, Anabolism, t Complex Protein 1"],"pubmed_title_synonyms":["Chaperonin Containing TCP 1, Chaperonin, gamma Subunit, Chaperonin CCT, determination, Testis Complex Polypeptide 1, beta Subunit, CCT delta Subunit, Subunit 2, alpha Subunit, Subunit 3, Subunit 1, Subunit 7, Subunit 4, t Complex Polypeptide 1, Subunit 5, Subunit 8, Concept, Cytosolic Chaperonin, t-Complex Protein 1, Chaperonin Containing TCP1, theta Subunit, Cytosolic, t-Complex Polypeptide 1, Role Concept, Roles, Chaperonin-Containing T-Complex Polypeptide 1, eta Subunit, chemical analysis, Role Concepts, Role, Concepts, Chaperonin Containing t Complex Polypeptide, Subunit 6A, epsilon Subunit, mTOR complex., assay, zeta2 Subunit, Cytosolic Molecular Chaperone CCT, Subunit 6B, Chaperonin Containing t-Complex Polypeptide, zeta1 Subunit, Chaperonin Containing T Complex Polypeptide 1, delta Subunit, t Complex Protein 1"],"name_synonyms":["DAX-1, POP3, LST8, CHM, DAX1, AA409454, CMT1E, NROB1, MPP, CMT1A, TORC subunit LST8, WAT1, CMT1B, AHX, G protein beta subunit-like, hmsnia, GAS-3, Spectrum Analyses, sp110, CG1049, Spectrum Analysis, lts8, Spectroscopy, Cct1, CCT1, DSS, MS, HNPP, DmelCG1049, SRXY2, p63, AI851821, Mass, CT41283, cct1, Sp110, hnpp, Analysis, Mammalian lethal with SEC13 protein 8, Gable, 0610033N12Rik, cmt1e, mLST8, cmt1a, Mass Spectrum Analyses, total congenital with posterior sutural opacities in heterozygotes, Mass Spectroscopy, c-cpn, TRic, AI528772, Mass Spectrum Analysis, Mass Spectrum, dss, HHG, GbetaL, Analyses, CMTDID, Gbl, GBL, AHCH, Spectrometry, CMT2I, CMT2J, CMT1, CMTDI3, P0, AI505104, cataract, gas-3, Tcp-1, CMT4E, gbl, Mass., Protein GbetaL, Ccta, CCT, Cct, GTD, total congenital, HMSNIA, HMSNIB, AHC, Tp63"],"citation_count":["0"],"additional_accession":["PXD013975"]},"is_claimable":true,"name":"mLST8-CCT DSS crosslinking mass spectrometry","description":"DSS crosslinking of mLST8-CCT purified from HEK293T cells using a tandem affinity approach. First cell extract was run over HisTrap column then a streptactin column.","dates":{"publication":"Wed May 22 13:54:00 BST 2019"},"accession":"MSV000083839","cross_references":{"pubmed":["31253771"]}}