MassIVEapplication/xmlftp://massive.ucsd.edu/v02/MSV000083884/primaryOK2000000ProteomicsHarm HogenEsch6410 Triple Quadrupole LC/MSMus Musculus (ncbitaxon:10090)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=f217a4dd7614407f85c93e732aa90e30hogenesch@purdue.eduPurdue UniversityMassIVE10,630Lipidomics analysisProSAP1, the integument, Biological Markers, Viral Marker, "dermatitis" EXACT [NCI2004_11_17:C2983], Surrogate Endpoints, pelt, ion, hindlimb zeudopodium, determination, Laboratory, Skn-li, Atopic neurodermatitis, leg, Mus domesticus, Atopic, NetrinA, posterior region of leg, D430049E23Rik, Biochemical, A4, Endpoint, CASP-14, protein, "Dermatitis NOS" EXACT [SNOMEDCT_2005_07_31:267847004], Serum, clefted, composed of, House Mouse, element, "dermatitis" EXACT [CSP2005:4008-0032], protein polypeptide chains, Laboratory Markers, sura, diseases, Biological, NOS" EXACT [MTHICD9_2006:692.9], diseases and disorders, Analysis, protein aggregate, (2Z)-but-2-enedioate, animal, sural region, WMS, integumentum commune, male, organ, lower extremity zeugopod, Progression, human disease, Elkh, Sex, crus of hindlimb, Man (Taxonomy), EK6, dermoid system, entire skin, composition, Swiss Mice, proteins, present in organism, outer epithelial layer, Sap-2, skin inflammation, Phenotypic, neuroendocrine tumour, reaction, lower extremity middle limb segment, Immune, zone of skin inflammation, sex, Markers, Solute carrier family 6 member 2, Besnier's prurigo, Viral Markers, Otf11, Fam91a1, CT27014, Exacerbation, NET1, SLC6A5, allergic dermatitis, Homo sapiens disease, "skin inflammation NOS" EXACT [SNOMEDCT_2005_07_31:156388001], stage, calf, associated, netA, GPHYSD2, AV220772, skin and subcutaneous tissue, middle limb segment of hind limb, posterior curral region, SGS, Elk, ELK, inflammatory skin disease, Viral, subdivided, Surrogate Endpoint, Neuronally-expressed EPH-related tyrosine kinase, CORTBP1, Modern, allergic form of dermatitis, mouse, Disease Exacerbation, SAP2, Biochemical Markers, integumental organ, Infantile, 9330129L11, Biologic Marker, female, hind epipodium, neuroendocrine neoplasm, ACMICD, Spectroscopy, inflammation of zone of skin, organism, "Other dermatitis due to solar radiation" EXACT [], Atopic dermatitis and related conditions (disorder), Ionen, increased period, Norepinephrine transporter, Marker, Inflammatory skin disease, BC033609, Lipid, Mini-ICE, regio cruris posterior, Diseases, precocious, EPH-like kinase 6, forked, posterior part of leg, Mus musculus, Skin inflammation, Genotypic, divided, whole organism, Caspase-14 subunit p10, End Points, maleate, mice, "skin inflammation NOS (disorder)" EXACT [SNOMEDCT_2005_07_31:267856007], content, "Dermatitis, Swiss Mouse, Spectrometry, Caspase-14 subunit p19, Eczema, MASS, Immunologic, hEK6, AUTS17, Laboratory Marker, MICE, septate, mKIAA0493, early, domesticus, Infantile Eczema, Atopic Dermatitis, disease, Dermatitides, DmelCG18657, vertebrate epidermis, DMDA, "Dermatitis NOS (disorder)" EXACT [SNOMEDCT_2005_07_31:200884006], Biochemical Marker, atopic eczema, Koerper, hindlimb zeugopod, Mouse, entire integument, dermal system, hypoderm, humans, AW488255, dermis plus epidermis plus hypodermis, lipids, 3.4.22.-, other disease, hindlimb zeugopodium, advanced, region of skin, multi-cellular organism, Other atopic dermatitis and related conditions, Clinical Markers, Otf-11, Dermatitis, Clinical Marker, developmental stage, FBN, mini-ICE, "Dermatitis (disorder) [Ambiguous]" EXACT [SNOMEDCT_2005_07_31:4979002], Atopic dermatitis, slow time, protein-containing complex, hind limb middle limb segment, TYPE, epidermis, Human, netrin, Surrogate End Points, regio surae, DAGA4, Surrogate Markers, polypeptide chain, Homo sapiens, House, Hek6, ECTOL1, zeugopod of hind limb, Clinical Progression, zeugopod of hindlimb, Mus musculus domesticus, disease or disorder, Cek6, Atopic Dermatitides, inflammation of skin, hindlimb epipodium, MAM, Mice, SCG3, hind limb zeudopodium, Disseminated Neurodermatitides, Man, ENSMUSG00000074119, Phenomenography, Skn-1a, Erp, APUDoma, ERP, Biomarker, CTTNBP1, SPANK-3, skin, Clinical, Genotypic Sex, Swiss, "Dermatitis (disorder)" EXACT [SNOMEDCT_2005_07_31:182782007], iones, Spectrum, Biological Marker, Tyrosine-protein kinase receptor EPH-2, lower leg, Maintenances, ions, SKIN, Inflammatory abnormality of the skin, non-neoplastic, OCTD, skin organ, crus, Immunologic Markers, 2.7.10.1, Clinical Course, anatomical unit, Etrp, body organ, inflammation of the skin, allergic, Chronic, disorder, NAT1, epidermis (sensu Metazoa), Immunologic Marker, Net, NET, Biologic, hind limb zeugopod, Disseminated., Atopic Neurodermatitides, Disease, C130099E04Rik, Oct-11a, atopic dermatitis and related conditions, female human body, body, protein complex, Males, Serum Markers, disorders, EPH tyrosine kinase 2, End Point, skin zone, intermediate segment of free lower limb, whole body, eczematous dermatitis, function, medical condition, eczema, compositionality, hypodermis, neuroendocrine tumor, increased time, net, chronic, OTHER ATOPIC DERMATITIS, LGMD2C, Immune Marker, skin plus hypodermis, Neurodermatitis, skin region, Oct11, native protein, natural protein, middle limb segment of hindlimb, Mus, Surrogate End Point, Protein, chemical analysis, high time, condition, MFS1, biological sex, Skin-1a, prolonged period, calf of leg, tegument, Skin, WMS2, Biologic Markers, "Dermatitis NOS" EXACT [SNOMEDCT_2005_07_31:156358005], Serum Marker, DMDA1, Surrogate, Endpoints, vertebrate integument, House Mice, Abnormal tendency to infections of the skin, Neurodermatitides, Disseminated, CG18657, Surrogate Marker, Epoc-1, Laboratory Mice, male human body, portion of skin, integument, posterior leg region, Ion, EPHT2, Phenotypic Sex, shank, Modern Man, structure, SCARMD2, SSKS, Disseminated Neurodermatitis, hindlimb middle limb segment, assay, outer epidermal layer, SHANK, Laboratory Mouse, Atopic Neurodermatitis, netrin A, "Other chronic dermatitis due to solar radiation" EXACT [], Females, Immune MarkersGenotypic, lipids, 3.4.22.-, Mus musculus, Sex, Genotypic Sex, Caspase-14 subunit p10, Laboratory, Swiss, mice, Mus domesticus, Swiss Mouse, mouse, RBCKL1, mini-ICE, House Mice, Swiss Mice, Caspase-14 subunit p19, CASP-14, cpdm, MICE, House Mouse, Phenotypic, Laboratory Mice, domesticus, Conneck1, sex, AW121341, House, Mus, Phenotypic Sex, SIPL1, 0610041B22Rik, Lipid, Mini-ICE, Mus musculus domesticus, biological sex, Mouse, Mice, Laboratory Mouse, Mus musculus.0trueInfluence of sex and disease stage on epidermal lipid profiles in SHARPIN-deficient miceSkin is an essential organ that preserves the integrity of the body and the maintenance of lipid content and composition is essential for proper epidermal barrier function. SHANK-associated RH domain interacting protein-deficient mice spontaneously develop a chronic proliferative dermatitis with similarities to atopic dermatitis in humans. To learn about changes in the epidermal lipid-content in male and female mice during disease progression, we used 18 male and 18 female wild-type mice and 3 groups of littermates mice with dermatitis (6 male and 6 female each) divided according to the stage of disease: non-lesional, established and advanced. A mass-spectrometry strategy for biomarker discovery termed multiple-reaction monitoring-profiling was used to detect and monitor 1030 lipid ions present in the epidermis samples. Univariate analysis was performed to link lipids to disease stage in a sex-dependent or independent manner. An elastic-net regression model was built using the top 10 lipid features to predict disease progression. Individual samples were classified into their corresponding disease stage groups with an accuracy of 0.90 (95% CI:0.86, 0.93). Multiple-reaction monitoring-profiling paired with machine-learning identified predictive biomarkers of dermatitis in mice and may provide the basis for a molecular diagnostic approach to atopic dermatitis.Mon Jun 03 12:43:00 BST 2019MSV000083884