{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v02/MSV000085056/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Leonard Foster"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=376ad345c5c74a29942bcc7997e01ba0"],"submitter_email":["foster@msl.ubc.ca"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["2,960"],"ptm_modification":["Oxidation [M]","Carbamidomethylation [C]","Acetylation [Protein N-term]"],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["Bruker Impact II Q-TOF"],"species":["Apis Mellifera"],"submitter_affiliation":["University of British Columbia"],"pubmed_abstract":["<h4>Background</h4>Queen failure is a persistent problem in beekeeping operations, but in the absence of overt symptoms it is often difficult, if not impossible, to ascertain the root cause. Stressors like heat-shock, cold-shock, and sublethal pesticide exposure can reduce stored sperm viability and lead to cryptic queen failure. Previously, we suggested candidate protein markers indicating heat-shock in queens. Here, we further investigate these heat-shock markers and test new stressors to identify additional candidate protein markers.<h4>Results</h4>We found that heat-shocking queens for upwards of 1 h at 40 °C was necessary to induce significant changes in the two strongest candidate heat-shock markers, and that relative humidity significantly influenced the degree of activation. In blind heat-shock experiments, we tested the efficiency of these markers at assigning queens to their respective treatment groups and found that one marker was sufficient to correctly assign queens 75% of the time. Finally, we compared cold-shocked queens at 4 °C and pesticide-exposed queens to controls to identify candidate markers for these additional stressors, and compared relative abundances of all markers to queens designated as 'healthy' and 'failing' by beekeepers. Queens that failed in the field had higher expression of both heat-shock and pesticide protein markers, but not cold-shock markers.<h4>Conclusions</h4>This work offers some of the first steps towards developing molecular diagnostic tools to aid in determining cryptic causes of queen failure. Further work will be necessary to determine how long after the stress event a marker's expression remains elevated, and how accurate these markers will be for field diagnoses."],"pubmed_title":["Candidate stress biomarkers for queen failure diagnostics."],"pubmed_authors":["McAfee Alison A, Milone Joseph J, Chapman Abigail A, Foster Leonard J LJ, Pettis Jeffery S JS, Tarpy David R DR"],"pubmed_title_synonyms":["Biomarker, Biological Markers, Viral Marker, Serum Marker, Viral, Surrogate Endpoints, Clinical, Surrogate Endpoint, End Points, Clinical Markers, Laboratory, Immune Markers., Surrogate, Clinical Marker, Biological Marker, Serum Markers, Endpoints, Biochemical, End Point, Endpoint, Biochemical Markers, Immunologic, Serum, Laboratory Marker, Biologic Marker, Surrogate Marker, Immune Marker, Surrogate End Points, Surrogate Markers, Laboratory Markers, Immunologic Markers, Immune, Markers, Biochemical Marker, Marker, Biological, Surrogate End Point, Viral Markers, Immunologic Marker, Biologic, Biologic Markers"],"pubmed_abstract_synonyms":["Antemortem Diagnosis, T22F8.160, degree (angle), and rna binding 2, Chronic Airflow Obstructions, Pb, Molecular Diagnostics, chronic obstructive pulmonary disease (COPD), protein, sci, Molecular Pathologies, GRO:0005338, Diagnosis, Long Term, Circulatory Collapse, Productivity, protein polypeptide chains, Donor Artificial Insemination, T22F8_160, Hot, ATGPR7, CDA2, chronic obstructive airways disease NOS (disorder), symptoms, Chronic irreversible airway obstruction, pathogenesis, HOW, How, Heat, GRP8, GRP7, Chronic Obstructive Pulmonary Disease (COPD), protein aggregate, preoperative procedures, Effect, l(3)j5D5, Donor Artificial, Shock, X-Chromosome-Bearing Sperm, 24B, treatment, sperm cell, X-Bearing, COLD (chronic obstructive lung disease), NEC in ICD9CM_2006, Molecular, developmental field, stru, CAFL - Chronic airflow limitation, proteins, l(3)S053606, CG10293, Chronic airflow limitation, l(3)j5B5, Y Chromosome Bearing Sperm, Y-Bearing Sperm, disease (COPD), GLYCINE RICH PROTEIN 7, climbing root (narrow), X Chromosome Bearing Sperm, Y-Chromosome-Bearing Sperms, Pathologies, disease management, Therapies, glycine-rich RNA-binding protein 8, CHRONIC OBSTRUCTIVE PULMONARY DISEASE, Long-Term Effects, Diagnose, ejaculate, CHRONIC, Therapy, Diagnostic Molecular Pathologies, screening, Y-Chromosome-Bearing, 0904/17, chronic obstructive lung disease [Ambiguous], obstructive lung disease, plomo, Blei, Longterm Effect, Molecular Diagnostic, aerial root (narrow), AV265756, plomb, 82Pb, Circulatory Failure, future organ, Airflow Obstructions, Diagnoses, CHRONIC OBSTRUCTIVE AIRWAY DIS, pulmonary disease (COPD), Postmortem, SZ1, CHRONIC OBSTRUCTIVE, Examinations and Diagnoses, chronic obstructive pulmonary disease and allied conditions, Chronic airway disease, Pathology, Heterologous Insemination, NOS, Postmortem Diagnosis, Airflow Obstruction, activation, Apiculture, COPD NOS, CHRONIC OBSTRUCTIVE LUNG DIS, GLYCINE-RICH PROTEIN 8, Chronic Obstructive Airways Disease, Diagnoses and Examination, Chronic airway obstruction, Chronic obstructive lung disease, X-Chromosome-Bearing, HTX2, Diagnostics, Postmortem Diagnoses, cold, Diagnostic Molecular, signs, root, spermatozoid, X Bearing Sperm, Treatments, Chronic Airflow Obstruction, seminal fluid, Artificial Insemination, F2G1.4, OBSTRUCTIVE PULMONARY DISEASE (COPD), COPD - Chronic obstructive pulmonary disease, Sperms, chronic obstructive airways disease, Hot Temperatures, anon-EST:Liang-2.39, obstructive pulmonary disease (COPD), Diagnoses., chronic obstructive lung disease, COLD, invasive procedures, Sperm, Effects, Circulatory, DISEASE (COPD), P62, CAO - Chronic airflow obstruction, Diagnostic Molecular Pathology, Gene, circadian rhythm, CHRONIC OBSTRUCTIVE PULM DIS, Chronic obstructive pulmonary disease NOS, Y-Chromosome-Bearing Sperm, protein-containing complex, AID, Aid, Failure, and RNA binding 1, Chronic obstructive pulmonary disease finding, Cold, organ field, PULMONARY DISEASE (COPD), Pulmonary Disease, polypeptide chain, Spermatozoon, Gene Products, field, Molecular Pathology, Antemortem, Y Bearing Sperm, aid, Insemination, Cold Temperatures, l(3)s2612, Diagnostic, Longterm, Pesticide, X-Bearing Sperms, causes, Long-Term, Diagnoses and Examinations, Chronic Obstructive, X-Chromosome-Bearing Sperms, Hypovolemic Shock, Hypovolemic, cryptic, ATGRP7, ATGRP8, sperm, HEL-S-284, NEC, lead, Humidities, causality, DmelCG10293, Chronic, HIGM2, SPERM, b2b970Clo, Long-Term Effect, operative procedures, Heterologous, COPD, Antemortem Diagnoses, COAD - Chronic obstructive airways disease, X-Bearing Sperm, findings, chronic obstructive airways disease NOS, protein complex, clone 2.39, not elsewhere classified, COAD, Proteins, intraoperative procedures, qkr, COLD - Chronic obstructive lung disease, l(3)S090417, chronic, PULM DIS CHRONIC OBSTRUCTIVE, Examination and Diagnoses, Chronic obstructive pulmonary disease finding (finding), spermatozoon, all, Human Donor, CFC1B, Temperatures, native protein, peroperative procedures, natural protein, KH93F, Protein, Long Term Effects, Donor, GLYCINE-RICH RNA-BINDING PROTEIN 7, (COPD), chronic airway obstruction, chronic obstructive, Y-Bearing, CAL - Chronic airflow limitation, who, chronic obstructive airway disease, Artificial, Temperature, Arp2, operative therapy, ARP2, Dops, plumbum, GR-RBP7, GR-RBP8, CRYPTIC, operations, arc degree, Who/How, Longterm Effects, Collapse, Protein Gene Products, Gene Proteins, Y-Bearing Sperms, Therapeutic, DTGA2, chronic obstructive lung disease (disorder), perioperative procedures, qkr[93F], cold (chronic obstructive lung disease), Treatment, Chronic Obstructive Lung Disease, chronic obstructive pulmonary disease"],"name_synonyms":["body fluid, fluid., Peptidomics"],"description_synonyms":["l(2)k04204, Ammonium, OG12X, Sperm, Peptidomics, Gene, Carbamide, 6330543G17Rik, Basodexan, fluid, Y-Chromosome-Bearing Sperm, [NH4](+), Buffer, l(2)k05821, Karbamid, ammonium cation, Polypeptides, peptido, l(2)k15606, reduced, azanium, Harnstoff, body fluid, Spermatozoon, Gene Products, tiny, Carmol, Y Bearing Sperm, Shot, X-Chromosome-Bearing Sperm, sperm cell, Apis mellifica, X-Bearing, l(2)k10821, peptides, E927b, ammonium ion, beta-Trypsin, Su(Mir)1, hypoplasia, X-Bearing Sperms, X-Chromosome-Bearing Sperms, Y Chromosome Bearing Sperm, Y-Bearing Sperm, sperm, X Chromosome Bearing Sperm, Y-Chromosome-Bearing Sperms, sample, honey bee, peptidos, l(2)CA4, SPERM, Prx3, Ammonium(1+), ejaculate, small, Y-Chromosome-Bearing, bee, X-Bearing Sperm, kop, Trypsin/K, Proteins, Liquid Chromatography, CG18076, NH4+, CG18637, buffer, ur, Peptide, shortstop/kakapo, spermatozoon, carbamide, NH4(+), carbonyldiamide, honeybee, DmelCG18076, Protein, Y-Bearing, l(2)k05434, X-Chromosome-Bearing, PRSS, ammonium, Grv, underdeveloped, uree, l(2)k03010, Tripcellim, kak, spermatozoid, beta Trypsin, SHOT, X Bearing Sperm, OG12, sample population, Protein Gene Products, seminal fluid, Gene Proteins, Trypure, Y-Bearing Sperms, H2NC(O)NH2, grv, Sperms, Peptid, Polypeptide, Og12x, anatomical systems., 42/4, 1728"],"citation_count":["0"],"additional_accession":["PXD017886"]},"is_claimable":false,"name":"Proteomics on spermathecal fluid of BC queens","description":"We sampled honey bee queens from beekeepers throughout British Columbia which were rated as either 'failed' or 'healthy' by beekeepers. We performed shot-gun proteomics on the spermathecal fluid (sperm removed). Proteins were reduced, alkylated, and digested with Lys-C for 4 h in urea digestion buffer. The sample was then diluted 4x with ammonium bicarbonated and digested overnight with trypsin. 2 ug of peptides were analyzed by an EASY-nLC 1000 liquid chromatography system coupled to a Bruker Impact II mass spectrometer. Data were searched with MaxQuant and the zipped txt folder output is provided.","dates":{"publication":"Wed Mar 04 19:39:00 GMT 2020"},"accession":"MSV000085056","cross_references":{"pubmed":["32819278"],"TAXONOMY":["7460"]}}