MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v02/MSV000085154/primaryOK2000000Anne-Claude Gingrashttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=c10a29632bc34515b4a7a2fa2c4d7511gingras@lunenfeld.caMassIVE1,422MOD:00685 - "A protein modification that effectively converts an L-glutamine residue to L-glutamic acid."MOD:00684 - "A protein modification that effectively converts an L-asparagine residue to L-aspartic acid."MOD:00719 - "A protein modification that oxygenates an L-methionine residue to one of the diastereomeric L-methionine sulfoxide residues."ProteomicsLTQ Orbitrap VelosLTQ Orbitrap EliteHomo Sapiens (ncbitaxon:9606)LTRIWe used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously annotated as mitochondrial, nearly half of the mitochondrial proteome defined by Mitocarta 2.0. Bait-bait analysis showed a clear separation of mitochondrial compartments, and correlation analysis among preys across all baits allowed us to identify functional clusters involved in diverse mitochondrial functions and to assign uncharacterized proteins to specific modules. We demonstrate that this analysis can assign isoforms of the same mitochondrial protein to different mitochondrial sub-compartments and show that some proteins may have multiple cellular locations. Outer membrane baits showed specific proximity interactions with cytosolic proteins and proteins in other organellar membranes, suggesting specialization of proteins responsible for contact site formation between mitochondria and individual organelles.A High-Density Human Mitochondrial Proximity Interaction Network.Antonicka Hana H, Lin Zhen-Yuan ZY, Janer Alexandre A, Aaltonen Mari J MJ, Weraarpachai Woranontee W, Gingras Anne-Claude AC, Shoubridge Eric A EA0PXD018196falseAntonicka_et_al_MitoMap_P129_2020This submission contains 283 raw mass spectrometry files and associated peak lists and result files for the manuscript by Hana Antonicka et al. that describes the high-density proximity mapping of mitochondria. Wed Mar 25 14:32:00 GMT 2020MSV00008515432877691