MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v03/MSV000085456/primaryOK2001000Mike KniermanRobert Siegelhttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=673248bc3fed46b090d7466fabd982f3siegel_robert@lilly.comknierman@lilly.comMassIVE29UNIMOD:303 - "Cysteine mercaptoethanol."UNIMOD:345 - "Cysteine oxidation to cysteic acid."UNIMOD:27 - "Pyro-glu from E."UNIMOD:351 - "Tryptophan oxidation to kynurenin."UNIMOD:425 - "Dihydroxy."MOD:00765 - "A protein modification that effectively converts an L-cysteine residue to S-(L-cysteinyl)-L-cysteine, forming a disulfide bond with free cysteine."UNIMOD:7 - "Deamidation."UNIMOD:374 - "Half of a disulfide bridge."UNIMOD:350 - "Tryptophan oxidation to hydroxykynurenin."UNIMOD:28 - "Pyro-glu from Q."UNIMOD:35 - "Oxidation or Hydroxylation."ProteomicsOrbitrap Fusion LumosSars Coronavirus (ncbitaxon:227859)Bos (ncbitaxon:9903)Homo Sapiens (ncbitaxon:9606)Eli Lilly and Co.Precise elucidation of the antigen sequences for T cell immunosurveillance greatly enhances our ability to understand and modulate humoral responses to viral infection or active immunization. Mass spectrometry is used to identify 526 unique sequences from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein extracellular domain in a complex with human leukocyte antigen class II molecules on antigen-presenting cells from a panel of healthy donors selected to represent a majority of allele usage from this highly polymorphic molecule. The identified sequences span the entire spike protein, and several sequences are isolated from a majority of the sampled donors, indicating promiscuous binding. Importantly, many peptides derived from the receptor binding domain used for cell entry are identified. This work represents a precise and comprehensive immunopeptidomic investigation with the SARS-CoV-2 spike glycoprotein and allows detailed analysis of features that may aid vaccine development to end the current coronavirus disease 2019 (COVID-19) pandemic.The Human Leukocyte Antigen Class II Immunopeptidome of the SARS-CoV-2 Spike Glycoprotein.Knierman Michael D MD, Lannan Megan B MB, Spindler Laura J LJ, McMillian Carl L CL, Konrad Robert J RJ, Siegel Robert W RWglicoproteinas, Co-Immunoprecipitations, COVID19, Dendritic Cells, Allelomorphs, O-Glycosylated, human being, mononuclear leukocyte, C-Glycosylated, Tandem, Interdigitating, COVID-19, 2019 novel coronavirus, HLA DRB1 Antigen, SARS CoV 2 Virus, Mass Spectrometry Mass Spectrometry, DRB1, COVID19 Virus, Human, Co Immunoprecipitation, Geographic, Polypeptides, Glycoprotein, peptido, SARS, Homo sapiens, glycoproteins, Novel Coronavirus, Virus, Antigen, Immune Precipitations, Interdigitating Dendritic Cell, β-coronavirus, Dendritic 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Wuhan, Active Immunizations, COVID-19 Pandemics, COVID-19 Pandemic, SARS-coronavirus 2, Protein Gene Products, extracellular, Gene Proteins, DmelCG17603, Vaccine, Modern Man, White Blood, Peptid, virulence, COVID 19 Virus, assay, ORW1, growth, White Blood Cell, COVID 19, TAF1mtSerRS, glicoproteinas, a glycoprotein, Interstitial Dendritic Cells, Interdigitating., SerRSmt, Dendritic Cells, Veiled Cells, glycated protein, O-Glycosylated, human being, C-Glycosylated, Interdigitating, Modern, Proteins, Interstitial, D7Ertd353e, Peptide, Cell, Mid(H15), Human, Polypeptides, Plasmacytoid Dendritic Cells, H15r, Glycoprotein, peptido, SARS, Homo sapiens, glycoproteins, SYS, SARSM, Protein, Glycosylated Proteins, Interdigitating Dendritic Cell, SERS, Dendritic Cell, glycoproteine, Interstitial Dendritic Cell, Man, H15r/nmr2, C-Glycosylated Proteins, SerRS, glicoproteina, Glykoprotein, N-Glycosylated, Plasmacytoid Dendritic Cell, Man (Taxonomy), peptides, Plasmacytoid Dendritic, Veiled Cell, nmr2, N-Glycosylated Proteins, Glycosylated, Interdigitating Dendritic, Dendritic, Veiled, Interdigitating Cells, los, glycoproteines, Plasmacytoid, CG6634, TBX20, extra, O-Glycosylated Proteins, 2410015F05Rik, human, DmelCG6634, Interstitial Dendritic, Prot/GlycoRes+, Modern Man, Cells, Interdigitating Dendritic Cells, Peptid, peptidos, Interdigitating Cell, glycosylated protein, Polypeptide, Glycosylated Protein, humans, Glykoproteine, Neoglycoproteinsglicoproteinas, a glycoprotein, COVID19, Leukocyte, glycated protein, SARS-CoV-2 Viruses, O-Glycosylated, human being, 2019 Novel Coronavirus, β-CoV, immune cell, C-Glycosylated, beta-CoV, COVID-19 Viruses, Modern, COVID-19, Proteins, SARS-CoV-2, 2019 novel coronavirus, Coronavirus 2, PMN cell, SARS CoV 2 Virus, White Blood Corpuscle, COVID19 Virus, Human, COVID19 Viruses, Severe Acute Respiratory Syndrome Coronavirus 2, Glycoprotein, Homo sapiens, SARS, glycoproteins, SARS-CoV-2 Virus, Protein, Glycosylated Proteins, Novel Coronavirus, Antigen, Virus, Corpuscles, Coronavirus, β-coronavirus, glycoproteine, Blood Corpuscle, Man, 2019-nCoV, C-Glycosylated Proteins, 2019 Novel Coronaviruses, Blood Cells, glicoproteina, Blood Cell, Glykoprotein, SARS Coronavirus 2, N-Glycosylated, leucocyte, β-CoVs, 2019 Novel, betacoronavirus, Neoglycoproteins., Man (Taxonomy), Viruses, N-Glycosylated Proteins, Coronavirus Disease 2019 Virus, Glycosylated, Wuhan Seafood Market Pneumonia Virus, glycoproteines, severe acute respiratory syndrome coronavirus 2, PMNC, Wuhan, White Blood Corpuscles, beta-CoVs, O-Glycosylated Proteins, human, SARS-coronavirus 2, White Blood Cells, white blood cell, Corpuscle, COVID-19 Virus, Prot/GlycoRes+, Modern Man, White Blood, 2019, White, COVID 19 Virus, Blood Corpuscles, glycosylated protein, Glycosylated Protein, White Blood Cell, humans, Wuhan Coronavirus, polymorphonuclear cell, Glykoproteine1falsePresentation of the Extra-Cellular Domain of the Sars2 Spike Glycoprotein by Immunopeptidomic Profiling of HLA-II Bound Peptides from Human Dendritic CellsIn the present study we sought to identify the naturally processed and presented immunopeptidome of the SARS-CoV-2 spike glycoprotein from human antigen presenting cells. Monocyte-derived dendritic cells from a panel of healthy human subjects representing a majority of the HLA-DRB1 allele usage within the United States were treated with recombinant spike glycoprotein. A subset of donors was also selected to represent common alleles from the Asia- Pacific geography. HLA II associated peptides were identified by liquid chromatography and nanoelectrospray ionization tandem mass spectrometry after immunoprecipitation. Thu May 21 23:50:00 BST 2020MSV00008545633220791