{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v03/MSV000085564/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Nathan A Yates"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=d425f0edd52a40f2b931013759d4a5ce"],"submitter_email":["yatesn@pitt.edu"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["24"],"ptm_modification":["MOD:01060 - \"A protein modification that effectively converts an L-cysteine residue to S-carboxamidomethyl-L-cysteine.\"","MOD:00719 - \"A protein modification that oxygenates an L-methionine residue to one of the diastereomeric L-methionine sulfoxide residues.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["LTQ Orbitrap XL"],"species":["Saccharomyces Cerevisiae By4741 (ncbitaxon:1247190)"],"submitter_affiliation":["University of Pittsburgh"],"description_synonyms":["other disease, Size, determination, GFP cell, number, Brewer's, baker's yeast, Spectrum Analyses, AID, supernumerary, Aid, Saccharomyces italicus, ACT2, AA409782, diseases, isolation, CDA2, yeast, ATARP2, GFP 细胞, Mass, DGS, disease or disorder, diseases and disorders, Sample Sizes, Analysis, lager beer yeast, Mass Spectroscopy, Mass Spectrum Analysis, increased, human disease, Analyses, gel, GFP, actin related protein 2, beta-Trypsin, Saccharomyes cerevisiae, purification, Baker, Saccharomyces uvarum var. melibiosus, free, S. cerevisiae, non-neoplastic, isolation and purification, DmelCG9901, ARABIDOPSIS RIBOSOMAL PROTEIN 2, S cerevisiae, Candida robusta, 4921510D23Rik, Saccharomyces capensis, DmelCG1106, HEL-S-284, sample, ARP14D, Baker's, disorder, HIGM2, Homo sapiens disease, Mycoderma cerevisiae, 1883, brewer's yeast, NICR1047, T25B24_7, actr2-A, Arp14D, Data Set, Saccharomyces cerevisiae 'var. diastaticus', disorders, T25B24.7, medical condition, Spectrum Analysis, Saccaromyces cerevisiae, Cell, arp2, Spectroscopy, Baker's Yeasts, Sccharomyces cerevisiae, Experiment, MS, arp2-A, Saccharomyces diastaticus, chemical analysis, Sizes, WURM, core, condition, R-protein L3 B, Mass Spectrum Analyses, Actr14D, Saccharomyces oviformis, Mass Spectrum, Yeast, PRSS, Arp2, D6Ertd746e, ARP2, increased number, CG9901, Tripcellim, Spectrometry, WRM, beta Trypsin, cardinality., RIBOSOMAL PROTEIN L3, sample population, CG1106, Trypure, present in greater numbers in organism, Saccharomyces cerevisiae (Desm.) Meyen ex E.C. Hansen, disease, Sample, label, ACTIN RELATED PROTEIN 2, assay, Baker's Yeast, SPAC22F8.01, Brewer's Yeast, Baker Yeast, accessory, END9"],"name_synonyms":["Mass Spectrum Analysis, isolation and purification, Spectroscopy, Mass Spectrum, Size, Sample, MS, isolation, Analyses, Mass, Mass., Sizes, Spectrometry, Sample Sizes, purification, Analysis, Spectrum Analyses, Mass Spectrum Analyses, Spectrum Analysis, Mass Spectroscopy"],"citation_count":["0"],"additional_accession":["PXD019656"]},"is_claimable":false,"name":"Evaluation of the effects of sample size on affinity purification-mass spectrometry","description":"An affinity purification experiment was carried out to evaluate the effects of sample size on the performance of AP-MS experiments. The Arp2/3 complex was affinity purified from Saccharomyces cerevisiae cells containing GFP-tagged Arc35, a core subunit of the complex, in-gel digested by trypsin, and label-free differential mass spectrometry was used for the unbiased analysis of detected molecular features. By comparing the performance of the full dataset (n=6 affinity purifications per condition) and resampled data subsets of different sample sizes (n=3 to 5 affinity purifications per condition), we showed that larger sample size not only increased the number of significant known interactors but also bettered their significance ranking. ","dates":{"publication":"Mon Jun 08 13:52:00 BST 2020"},"accession":"MSV000085564","cross_references":{}}