{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/x01/MSV000085761/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":7,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Andrea Sinz"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=a4e8b90abdd34c7089dec03f19adaf76"],"submitter_email":["andrea.sinz@pharmazie.uni-halle.de"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["14"],"ptm_modification":["MOD:00397 - \"A protein modification that is produced by reaction with iodoacetamide, usually replacement of a reactive hydrogen with a methylcarboxamido group.\"","UNIMOD:765 - \"Removal of initiator methionine from protein N-terminus.\"","UNIMOD:4 - \"Iodoacetamide derivative.\"","UNIMOD:766 - \"Removal of initiator methionine from protein N-terminus, then acetylation of the new N-terminus.\"","UNIMOD:7 - \"Deamidation.\"","UNIMOD:1 - \"Acetylation.\"","UNIMOD:35 - \"Oxidation or Hydroxylation.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["Orbitrap Fusion"],"species":["Homo Sapiens (ncbitaxon:9606)"],"submitter_affiliation":["Martin-Luther University Halle-Wittenberg Institute of Pharmacy Department of Pharmaceutical Chemistry & Bioanalytics Charles Tanford Protein Center"],"pubmed_abstract":["Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. The protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Data are available via ProteomeXchange with identifier PXD019423."],"pubmed_title":["Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients."],"pubmed_authors":["Ihling Christian C, Tänzler Dirk D, Hagemann Sven S, Kehlen Astrid A, Hüttelmaier Stefan S, Arlt Christian C, Sinz Andrea A"],"name_synonyms":["mtSerRS, SerRS, SerRSmt, SARS coronavirus caused disease or disorder, determination, SARS coronavirus infectious disease, severe acute respiratory syndrome, Proteins, SARS-CoV infection, Gene, patient, SARS-associated coronavirus, SARS ASSOC CORONAVIRUS, Sars1, Protein Gene Products, SARS coronavirus disease or disorder, Gene Proteins, Strs, serRS, Acute, Client., acute respiratory coronavirus infection, SARS, Patient, SYS, Clients, chemical analysis, SARSM, Protein, Severe acute respiratory syndrome (disorder), Gene Products, SERRS, assay, SERS, Respiratory Syndrome, Severe, Severe Acute"],"pubmed_title_synonyms":["COVID19, Disease, 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2019 Virus, Spectrometry, Wuhan, Methodological, COVID-19 Pandemics, COVID-19 Pandemic, study protocol, Methodological Study, beta-Ketopropane, SARS-coronavirus 2, plan specification, Protein Gene Products, Gene Proteins, disease, Patient, 2019, 2019 Novel Coronavirus Infection, Animal Viruses, Peptid, COVID 19 Virus, 2019-nCoV Diseases, assay, Polypeptide, COVID 19, methodology"],"citation_count":["7"],"additional_accession":["PXD019423"]},"is_claimable":false,"name":"MS analysis of SARS-CoV2 proteins from patient samples","description":"We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. Our protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. https://biorxiv.org/cgi/content/short/2020.04.18.047878v1","dates":{"publication":"Thu Jul 16 08:50:00 BST 2020"},"accession":"MSV000085761","cross_references":{"pubmed":["32568543"]}}