{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v03/MSV000085945/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Karima Schwab"],"instrument_platform":["LTQ Orbitrap XL"],"species":["Homo Sapiens (ncbitaxon:9606)","Mus Musculus (ncbitaxon:10090)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=e50e8f39be94497db47a71a45a3becaf"],"submitter_email":["karima.schwab@charite.de"],"submitter_affiliation":["Charite Universitaetsmedizin Berlin"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["37"],"ptm_modification":["UNIMOD:21 - \"Phosphorylation.\""],"data_protocol":[""],"pubmed_abstract":["Synapse loss is associated with motor and cognitive decline in multiple neurodegenerative disorders, and the cellular redistribution of tau is related to synaptic impairment in tauopathies, such as Alzheimer's disease and frontotemporal dementia. Here, we examined the cellular distribution of tau protein species in human tau overexpressing line 66 mice, a transgenic mouse model akin to genetic variants of frontotemporal dementia. Line 66 mice express intracellular tau aggregates in multiple brain regions and exhibit sensorimotor and motor learning deficiencies. Using a series of anti-tau antibodies, we observed, histologically, that nonphosphorylated transgenic human tau is enriched in synapses, whereas phosphorylated tau accumulates predominantly in cell bodies and axons. Subcellular fractionation confirmed that human tau is highly enriched in insoluble cytosolic and synaptosomal fractions, whereas endogenous mouse tau is virtually absent from synapses. Cytosolic tau was resistant to solubilization with urea and Triton X-100, indicating the formation of larger tau aggregates. By contrast, synaptic tau was partially soluble after Triton X-100 treatment and most likely represents aggregates of smaller size. MS corroborated that synaptosomal tau is nonphosphorylated. Tau enriched in the synapse of line 66 mice, therefore, appears to be in an oligomeric and nonphosphorylated state, and one that could have a direct impact on cognitive function."],"pubmed_title":["Differential compartmental processing and phosphorylation of pathogenic human tau and native mouse tau in the line 66 model of frontotemporal dementia."],"pubmed_authors":["Lemke Nora N, Melis Valeria V, Lauer Dilyara D, Magbagbeolu Mandy M, Neumann Boris B, Harrington Charles R CR, Riedel Gernot G, Wischik Claude M CM, Theuring Franz F, Schwab Karima K"],"description_synonyms":["the brain, suprasegmental structures, human being, TAU, Tau, Laboratory, Mtapt, Modern, Mus domesticus, mouse, PPND, Thermolysin S., House Mouse, encephalon, extracted material, AW045860, Human, Electrophoreses, Homo sapiens, tau, House, Mus, AI551861, DDPAC, Fractions, Mus musculus domesticus, MSTD, synganglion, Mice, Man, CKIe, Mus musculus, PRSS, Man (Taxonomy), Subcellular, Swiss, mice, Encephalon, Swiss Mouse, beta-Trypsin, Tripcellim, Bacillus thermoproteolyticus neutral proteinase, House Mice, Swiss Mice, FTDP-17, CK1epsilon, suprasegmental levels of nervous system, beta Trypsin, human, domesticus, Laboratory Mice, AW457082, Trypure, AI426939, Fraction, Subcellular Fraction, Modern Man, MAPTL, PPP1R103, KC1epsilon, TG, Mouse, house mouse, Laboratory Mouse, MTBT1, AI413597, humans, MTBT2"],"name_synonyms":["Familial Pick's Disease, Disinhibition-Dementia-Parkinsonism-Amyotrophy, frontotemporal lobar Degeneration with Tau inclusions, human being, Familial Picks Disease, Frontotemporal Lobe (FLDEM), Multiple System Tauopathy with Presenile Dementia, frontotemporal dementia, with Parkinsonism., Wilhelmsen-Lynch Diseases, AW045860, Human, HDDD2, HDDD1, GRN-Related Frontotemporal Dementias, Disinhibition-Dementia-Parkinsonism-Amytrophy, Hereditary Dysphasic Disinhibition Dementia, Homo sapiens, Pick's Disease, DDPAC, Complices, Familial Pick Disease, FTD, dementia, Wilhelmsen-Lynch, Pick's Diseases, Man, Disinhibition-Dementia-Parkinsonism-Amyotrophy Complex, with Parkinsonism, Ubiquitin Positive, Frontotemporal Dementia with Parkinsonism, Disinhibition-Dementia-Parkinsonism-Amytrophy Complices, Man (Taxonomy), Frontotemporal Lobe, Complex, GRN-Related Frontotemporal, Familial, CK1epsilon, FTLD-17 GRN, AW457082, Ubiquitin-Positive Frontotemporal Dementia, AI426939, Hereditary Dysphasic Disinhibition, FTD-PGRN, Frontotemporal Lobe Dementias, frontotemporal dementia with Parkinsonism, PPP1R103, KC1epsilon, Wilhelmsen Lynch Disease, Pallidopontonigral Degeneration, MTBT1, AI413597, MTBT2, Disease, Semantic, TAU, Tau, Mtapt, Modern, PPND, multiple system tauopathy with presenile dementia, Frontotemporal Lobe Dementias (FLDEM), Frontotemporal Lobe Dementia (FLDEM), GRN-Related, Frontotemporal Dementia with Parkinsonism-17, Ubiquitin-Positive Frontotemporal, Wilhelmsen-Lynch Disease, disinhibition-dementia-Parkinsonism-amyotrophy Complex, Frontotemporal Dementia, Semantic Dementias, Disinhibition-Dementia-Parkinsonism-Amytrophy Complex, frontotemporal lobe dementia, Ftdp17, tau, Frontotemporal Dementia with Parkinsonism 17, AI551861, Ubiquitin-Positive Frontotemporal Dementias, Frontotemporal Lobe Dementia, Diseases, MSTD, FTD-GRN, Familial Pick's, Lobe Dementias, Ubiquitin-Positive, Wilhelmsen-Lynch disease, frontotemporal, CKIe, Dementia, GRN-Related Frontotemporal Dementia, GRN Related Frontotemporal Dementia, Frontotemporal Lobar Degeneration With Ubiquitin-Positive Inclusions, Disinhibition Dementia Parkinsonism Amyotrophy Complex, Disinhibition-Dementia-Parkinsonism-Amyotrophy Complices, FTLD with TDP-43 Pathology, Frontotemporal Dementias, Frontotemporal, Dementias, Ftld with Tau inclusions, FTDP-17, FTLD with TDP 43 Pathology, Semantic Dementia, human, Disinhibition Dementia Parkinsonism Amytrophy Complex, Familial Pick's Diseases, Pick Complex, Modern Man, MAPTL, frontotemporal lobe dementia (FLDEM), Lobe Dementia, FTLD-TDP, humans, Frontotemporal Lobar Degeneration With Ubiquitin Positive Inclusions"],"pubmed_abstract_synonyms":["Familial Pick's Disease, Disinhibition-Dementia-Parkinsonism-Amyotrophy, Cognitive Function, Presenile Alzheimer Dementia, Triton X-45, ALZHEIMERS DIS, frontotemporal lobar Degeneration with Tau inclusions, Disorders, nucleocytoplasm, Mental deterioration, frontotemporal dementia, Laboratory, Early Onset, supply, Mus domesticus, Mild, Triton X-305, Cognitive Dysfunctions, Octoxinols, Triton X305, CASP-14, Deteriorations, Wilhelmsen-Lynch Diseases, Basodexan, Early Onset Alzheimer Disease, House Mouse, Alzheimer's disease, Poly(oxy-1, Primary Senile Degenerative, AD, Disinhibition-Dementia-Parkinsonism-Amytrophy, unspecified, Hereditary Dysphasic Disinhibition Dementia, diseases, Pick's Disease, DDPAC, Complices, 1, Synapse, Octoxynol 9, diseases and disorders, 3, synganglion, dementia, with Parkinsonism, Frontotemporal Dementia with Parkinsonism, multicellular organismal biosynthetic process, insoluble, imprinted and ancient gene protein, Octoxynol-9, treatment, LATE ONSET ALZHEIMER DIS, single-organism biosynthetic process, human disease, Familial Alzheimer Disease (FAD), Alzheimer Diseases, Man (Taxonomy), DAT - Dementia Alzheimer's type, anabolism, Frontotemporal Lobe, E927b, Cognitive decline, GRN-Related Frontotemporal, Cognitive Disorders, Swiss Mice, Familial, Focal Onset Alzheimer's Disease, suprasegmental levels of nervous system, Cell Bodies, Late Onset Alzheimer Disease, genetic, Ubiquitin-Positive Frontotemporal Dementia, Cognitions, Hereditary Dysphasic Disinhibition, Axon, AD - Alzheimer's disease, Functions, Frontotemporal Lobe Dementias, Alzheimer Type Dementia, PPP1R103, disease management, Therapies, Homo sapiens disease, Wilhelmsen Lynch Disease, TG, ALZHEIMER DIS EARLY ONSET, Deterioration, house mouse, Presenile, AI413597, Alzheimers disease, intracellular, Octylphenoxy Polyethoxyethanol, Therapy, Senile, Alzheimer disease, suprasegmental structures, Cognitive Decline, Acute Confusional Senile Dementia, Cognitive Impairment, Mtapt, Modern, familial, mouse, Impairments, Frontotemporal Lobe Dementias (FLDEM), antibodies, Triton X100, Alzheimer's Dementia, Frontotemporal Dementia with Parkinsonism-17, ur, Ubiquitin-Positive Frontotemporal, Triton X-100, Alzheimer Type, Semantic Dementias, Disinhibition-Dementia-Parkinsonism-Amytrophy Complex, carbamide, soluble, Mild Cognitive Impairment, AI551861, Tauopathy, Ubiquitin-Positive Frontotemporal Dementias, Mini-ICE, Diseases, MSTD, mixed synapse, NOS, Dysfunction, Familial Pick's, internal to cell, Lobe Dementias, Wilhelmsen-Lynch disease, Dementia, GRN-Related Frontotemporal Dementia, Frontotemporal Lobar Degeneration With Ubiquitin-Positive Inclusions, Mus musculus, Disinhibition Dementia Parkinsonism Amyotrophy Complex, Disinhibition-Dementia-Parkinsonism-Amyotrophy Complices, Alzheimers, FTLD with TDP-43 Pathology, Caspase-14 subunit p10, Mental Deterioration, Alzheimer, mice, Encephalon, Swiss Mouse, Dementias, Caspase-14 subunit p19, FTDP-17, Alzheimer-Type (ATD), Semantic Dementia, MICE, Treatments, human, Disinhibition Dementia Parkinsonism Amytrophy Complex, Progressive cognitive decline, domesticus, Familial Pick's Diseases, disease, synaptic junction, H2NC(O)NH2, Senile Dementia, Acute Confusional, MAPTL, Alzheimer-Type Dementia (ATD), frontotemporal lobe dementia (FLDEM), electrotonic synapse, Mouse, inherited genetic, Alzheimer's Disease, 3-tetramethylbutyl)phenyl)-omega-hydroxy-, Lobe Dementia, FTLD-TDP, progressive, E430016J11Rik, 1728, Polyethoxyethanol, Mental Deteriorations, humans, Alzheimer Type Dementia (ATD), Dementia of the Alzheimer's type, Frontotemporal Lobar Degeneration With Ubiquitin Positive Inclusions, the brain, Mental, 3.4.22.-, other disease, Impairment, human being, Familial Picks Disease, Frontotemporal Lobe (FLDEM), Alzheimers Diseases, Multiple System Tauopathy with Presenile Dementia, Bodies, Sclerosis, mini-ICE, Carbamide, biosynthesis, Dysfunctions, Focal Onset, Ximpact, 2-ethanediyl), AW045860, unspecified (disorder), HDDD2, Human, ALZHEIMER DIS, Karbamid, HDDD1, GRN-Related Frontotemporal Dementias, Octoxynols, Cognitive Declines, [X]Dementia in Alzheimer's disease, Homo sapiens, House, Octylphenoxy, Triton X 305, Harnstoff, disease or disorder, Mus musculus domesticus, Familial Pick Disease, FTD, Carmol, Wilhelmsen-Lynch, Pick's Diseases, Mice, Disinhibition-Dementia-Parkinsonism-Amyotrophy Complex, Man, Ubiquitin Positive, protoplasm, Disinhibition-Dementia-Parkinsonism-Amytrophy Complices, tau Protein, protoplast, formation, distribution, Swiss, Complex, CK1epsilon, alpha-(4-(1, FOCAL ONSET ALZHEIMERS DIS, FTLD-17 GRN, non-neoplastic, Presenile Dementia, synthesis, Cognitive Functions, AW457082, AI426939, Octylphenoxypolyethoxyethanols, Triton X45, Alzheimer Disease, FTD-PGRN, Disorder, frontotemporal dementia with Parkinsonism, KC1epsilon, disorder, Alzheimer's disease (disorder), species, Pallidopontonigral Degeneration, Cognitive Disorder, Alzheimers Dementia, constitutitional genetic, MTBT1, MTBT2, Alzheimer Senile Dementia, Mild Cognitive Impairments, Disease, Mild Cognitive, Semantic, TAU, Tau, Alzheimer Syndrome, Proteins, Octoxinol, disorders, PPND, multiple system tauopathy with presenile dementia, medical condition, Frontotemporal Lobe Dementia (FLDEM), GRN-Related, encephalon, Cell, impact-a, Primary Senile Degenerative Dementia, Wilhelmsen-Lynch Disease, cognitive function, [X]Dementia in Alzheimer's disease (disorder), disinhibition-dementia-Parkinsonism-amyotrophy Complex, Alzheimer Dementias, Frontotemporal Dementia, Insights, frontotemporal lobe dementia, Triton X 100, Ftdp17, tau, Frontotemporal Dementia with Parkinsonism 17, Mus, carbonyldiamide, Cognitive Impairments, Alzheimer's Diseases, Protein, Frontotemporal Lobe Dementia, condition, IMPACT, imprinted and ancient gene protein homolog, FTD-GRN, supply and distribution, Ubiquitin-Positive, frontotemporal, CKIe, GRN Related Frontotemporal Dementia, Exhibit, Frontotemporal Dementias, uree, Dementia in Alzheimer's disease, Triton X 45, Intellectual deterioration, Frontotemporal, Function, Alzheimer's Disease Pathway, Alzheimer Type Senile Dementia, House Mice, Ftld with Tau inclusions, FTLD with TDP 43 Pathology, Cognitive, Alzheimer Dementia, Body, Alzheimer Sclerosis, Laboratory Mice, Declines, Pick Complex, Late Onset, Therapeutic, Familial (FAD), Modern Man, Dementia in Alzheimer's disease (disorder), Insight., Decline, Treatment, Familial Alzheimer Diseases (FAD), hereditary, Laboratory Mouse, RWDD5"],"pubmed_title_synonyms":["Familial Pick's Disease, Disinhibition-Dementia-Parkinsonism-Amyotrophy, frontotemporal lobar Degeneration with Tau inclusions, human being, Familial Picks Disease, Frontotemporal Lobe (FLDEM), Multiple System Tauopathy with Presenile Dementia, frontotemporal dementia, Laboratory, Mus domesticus, with Parkinsonism., Wilhelmsen-Lynch Diseases, House Mouse, phosphorylation, AW045860, Human, HDDD2, HDDD1, GRN-Related Frontotemporal Dementias, Disinhibition-Dementia-Parkinsonism-Amytrophy, Hereditary Dysphasic Disinhibition Dementia, Homo sapiens, House, Pick's Disease, DDPAC, Mus musculus domesticus, Complices, Familial Pick Disease, FTD, dementia, Mice, Wilhelmsen-Lynch, Pick's Diseases, Man, Disinhibition-Dementia-Parkinsonism-Amyotrophy Complex, with Parkinsonism, Ubiquitin Positive, Frontotemporal Dementia with Parkinsonism, Disinhibition-Dementia-Parkinsonism-Amytrophy Complices, Man (Taxonomy), Swiss, Frontotemporal Lobe, Complex, GRN-Related Frontotemporal, Swiss Mice, Familial, CK1epsilon, FTLD-17 GRN, AW457082, Ubiquitin-Positive Frontotemporal Dementia, AI426939, Hereditary Dysphasic Disinhibition, FTD-PGRN, Frontotemporal Lobe Dementias, frontotemporal dementia with Parkinsonism, PPP1R103, KC1epsilon, Wilhelmsen Lynch Disease, house mouse, Pallidopontonigral Degeneration, MTBT1, AI413597, MTBT2, Disease, Semantic, TAU, Tau, Mtapt, Modern, mouse, Phosphorylations, PPND, multiple system tauopathy with presenile dementia, Frontotemporal Lobe Dementias (FLDEM), Frontotemporal Lobe Dementia (FLDEM), GRN-Related, Frontotemporal Dementia with Parkinsonism-17, Ubiquitin-Positive Frontotemporal, Wilhelmsen-Lynch Disease, disinhibition-dementia-Parkinsonism-amyotrophy Complex, Frontotemporal Dementia, Semantic Dementias, Disinhibition-Dementia-Parkinsonism-Amytrophy Complex, frontotemporal lobe dementia, Ftdp17, tau, Mus, Frontotemporal Dementia with Parkinsonism 17, AI551861, Ubiquitin-Positive Frontotemporal Dementias, Frontotemporal Lobe Dementia, Diseases, MSTD, FTD-GRN, Familial Pick's, Lobe Dementias, Ubiquitin-Positive, Wilhelmsen-Lynch disease, frontotemporal, CKIe, Dementia, GRN-Related Frontotemporal Dementia, GRN Related Frontotemporal Dementia, Mus musculus, Frontotemporal Lobar Degeneration With Ubiquitin-Positive Inclusions, Disinhibition Dementia Parkinsonism Amyotrophy Complex, Disinhibition-Dementia-Parkinsonism-Amyotrophy Complices, FTLD with TDP-43 Pathology, Frontotemporal Dementias, mice, Swiss Mouse, Frontotemporal, House Mice, Dementias, Ftld with Tau inclusions, FTDP-17, FTLD with TDP 43 Pathology, Semantic Dementia, human, Disinhibition Dementia Parkinsonism Amytrophy Complex, domesticus, Laboratory Mice, Familial Pick's Diseases, Pick Complex, Modern Man, MAPTL, frontotemporal lobe dementia (FLDEM), Mouse, Lobe Dementia, FTLD-TDP, Laboratory Mouse, humans, Frontotemporal Lobar Degeneration With Ubiquitin Positive Inclusions"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"Pathogenic human tau in the Line 66 model of frontotemporal dementia","description":"Transgenic human tau in subcellular fractions extracted after electrophoresis of L66 mouse brain extract. For higher sequence coverage trypsin (Tr) and thermolysin (TL) digests were analysed. ","dates":{"publication":"Thu Aug 13 05:18:00 BST 2020"},"accession":"MSV000085945","cross_references":{"pubmed":["33127647"]}}