{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v03/MSV000086045/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Erik Soderblom"],"instrument_platform":["Q Exactive HF"],"species":["Mus Musculus (ncbitaxon:10090)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=c667f5e3a3924603bee0acc69a40c6ba"],"submitter_email":["es114@duke.edu"],"submitter_affiliation":["Duke Univ"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["49"],"ptm_modification":["UNIMOD:35 - \"Oxidation or Hydroxylation.\""],"data_protocol":[""],"description_synonyms":["Water, sodium salt, Adh-1e, ammonium formate, 13C-labeled, MeCN, cadmium salt, ion, fond, H(2)O, AVSD3, BOUND WATER, magnesium formate, CycEI, ORE14, oxidane, zinc salt, cobalamin-dependent methionine synthase activity, Long Term, WATER, HOH, Polypeptides, %, M(3L)i, 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase activity, ERV1, cobalt(II) formate dihydrate, Method, AT5G62010, CH3-C#N, B1, 2-amino-3-mercaptopropanoic acid, Adh-1t, WMS, C79691, C, HPO, Adh-3e, F, HERV1, Data Collection, M, ADH1, Saccharomyes cerevisiae, procedures, Saccharomyces uvarum var. melibiosus, EG:196F3.2, AI047805, Lccp, column, sample, D1, GJAL, D9, M(3)RpS17, nickel salt, SGS, LC-MS2, cobalt (+2) salt, Longterm Effect, Procedure, Cyc E, ACMICD, ADH-AA, H2O, sodium (4:1:1) salt, BG:DS07108.3, Dm1, HSS, magnesium salt, M(3)i(55), Algorithm, AI194826, N-methyltetrahydrofolate:L-homocysteine methyltransferase activity, l(2)05206, (2R)-2-amino-3-sulfanylpropanoic acid, Hydrogen Oxide, rpS17, formate, cromium (+3), HARP, MASCOT, Methodological, Speed, HPO2, liquid chromatography-tandem mass spectroscopy, HPO1, ammonium (4:1) salt, methyltetrahydrofolate--homocysteine vitamin B12 methyltransferase activity, methyl cyanide, DmelCG32810, lead (+2) salt, Collection Method, MTG10_1, nickel formate dihydrate, cycline, lead formate, Effects, False, Biocatalysts, DmcyclinE, aluminum salt, number, L-Zystein, HLHS1, precursor, Chalk, Saccharomyces italicus, CG7826, agua, cdi7, M(3)i, cyclinE, LC-MSMS, yeast, CG7835, Cdi7, CDI7, Gene Products, CG42273, ODDD, Technique, lager beer yeast, F15E12_6, tetrahydrofolate methyltransferase activity, anatomical systems, thallium (+1) salt, Longterm, ARF1-BP, Dual Data, E-920, rubidium salt, Sciences, Hcys, Long-Term, methanoic acid, l(3)dtOA4, DmcycE, ions, ORESARA 14, Study, Injectable, mKIAA0989, Hallermann Streiff Francois syndrome, Enzyme, CYSTEINE, M(3)i[55], FREE CYSTEINE, peptidos, (2R)-2-amino-3-mercaptopropanoic acid, PTHB1, ALR, 1-(14)C-labeled, Proteins, potassium formate, copper (+2) salt, backward, acetonitrile, Saccaromyces cerevisiae, Plasma Albumin, polypeptide, Sccharomyces cerevisiae, l(2)k02514, Temperatures, DmCycE, C18, CX43, cupric formate, chemical analysis, Long Term Effects, HLS1 SUPPRESSOR, BcDNA:RE44119, MFS1, aluminum formate, Collection, ATCMPG1, MET, ATCMPG2, Saccharomyces oviformis, AU020952, lithium formate, l(2)k02602, ARF1-BINDING PROTEIN, M(3)q, N5-methyltetrahydrofolate methyltransferase activity, (R)-2-amino-3-mercaptopropanoic acid, ethanenitrile, muscle, anon-EST:Posey48, N(5)-methyltetrahydrofolic--homocysteine vitamin B12 transmethylase activity, primary structure of sequence macromolecule, 2-Amino-3-mercaptopropionic acid, Longterm Effects, ME-IV, D-CycE, Gene Proteins, data collection, M(3L)i[55], liquid chromatography tandem mass spectrometry, E920, [OH2], reversed, liquid chromatography tandem mass spectroscopy, Rps17, E 920, Meth, NCMe, determination, zinc formate, RPS17, lead salt, Serum, M(3)67, peptide, Techniques, ammonium tetraformate, Ccne, peptido, MetH, Min, auxin response factor 2, Cystein, 5-methyltetrahydrofolate--homocysteine transmethylase activity, Effect, Injectables, Adh3-e, DmelCG42273, B12 N5-methyltetrahydrofolate homocysteine methyltransferase activity, N5-methyltetrahydrofolic-homocysteine vitamin B12 transmethylase activity, LCMSMS, peptides, MUB3_18, cisteina, l(3)67BDo, min, mAPC, copper, MUB3.18, SCAN, lithium salt, CMDR, Methodological Studies, Saccharomyces capensis, Ice, ppm, dihydridooxygen, dihydrogen oxide, brewer's yeast, Adh-1-t, GPHYSD2, ammonium (2:1) salt, Long-Term Effects, Hallermann Streiff syndrome, PRO, LC-MS/MS, aqua, Dmel_CG7826, M(3)67C, DFNB38, br37, CYS, Cys, B12 N(5)-methyltetrahydrofolate homocysteine methyltransferase activity, C20orf48, Ionen, CG14784, nickel (+2) salt, and GLY protein 2, Dmel_CG7835, CG14783, Crystal, Mnb, MNB, parent ion, ammonium salt, Yeast, tetrahydropteroylglutamic methyltransferase activity, and GLY protein 1, Collection Methods, Rp S17, hydrogen hydroxide, cobaltous formate, 5-methyltetrahydrofolate-homocysteine S-methyltransferase activity, MASS, AW124434, Methodological Study, acqua, muscle element, L-Cystein, nanospray, l35Dd, precursor ion, copper salt, Biocatalyst, Wasser, Dual Data Collection, Polypeptide, Procedures, AW549739, cesium salt, FBN, Gene, baker's yeast, cycE, aligned, presence, l(2)br37, LC-MS-MS, CYCLE, formic acid, potassium salt, M(3)S33, Injection, Adh-1, ECTOL1, 14C-labeled, Tina, Studies, F15E12.6, MTG10.1, Data Collection Method, methionine synthase (cobalamin-dependent) activity, N(5)-methyltetrahydrofolate methyltransferase activity, CYCE, Glass, PKAN, iones, DmelCG3938, CyclE, 3938, Minute, eau, Adh1tl, calcium formate, OCTD, l(2)k05007, N(5)-methyltetrahydrofolate--homocysteine cobalamin methyltransferase activity, dm-cycE, Candida robusta, DmelCG3922, 10^[-6], musculus, N5-methyltetrahydrofolate-homocysteine cobalamin methyltransferase activity, cromium (+3) salt, Long-Term Effect, tetrahydropteroylglutamate methyltransferase activity, False., sodium formate, DYRK1, 5E4, Data Collection Methods, nickel formate, 3H-labeled, NBIA1, strontium formate, aligned to, strontium salt, Peptide, count in organism, LC/MS/MS, water, Protein, Adh1-t, ADC1, sequence, Dyrk1, techniques, CyeE, Adh1-e, ACETONITRILE, Zystein, cyanomethane, Data Base, WMS2, Methods, 2-amino-3-sulfanylpropanoic acid, CC1, l(2)35Dd, L-2-Amino-3-mercaptopropionic acid, sample population, chromic formate, CG3922, Protein Gene Products, Albumin, Ion, Data, cardinality, SSKS, vitamin B12 methyltransferase activity, calcium salt, Peptid, assay, variable, CG3938, CG32810, 5-methyltetrahydrofolate--homocysteine S-methyltransferase activity, methodology"],"name_synonyms":["Hsf1alpha, D-HSF, xhsf1, DmHSF, DmHSFa, EXA3, HSFa, Dm-Hsf, HSTF1, dHSFa, DmelCG5748, l(2)03091, CG5748., AA960185, Hsf1beta, MAS3, HSF1, dHSF, Hsf, hsf, HSF, hstf1"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"Thiele HSF1 C HS HSR Interactome ","description":"Methods. Quantitative LCMSMS was performed on 4 uL of each sample using a nanoAcquity UPLC system Waters Corp coupled to a Thermo QExactive HF high resolution accurate mass tandem mass spectrometer Thermo via a nanoelectrospray ionization source. Briefly the sample was first trapped on a  Symmetry C18 20 mm x 180 um trapping column 5 ul min at 99.9 0.1 vv water acetonitrile after which the analytical separation was performed using a 1.8 um Acquity HSS T3 C18 75 um x 250 mm column Waters Corp. with a 90min linear gradient of 5 to 30 acetonitrile with 0.1 percent formic acid at a flow rate of 400 nanoliters minute nL min with a column temperature of 55C. Data collection on the QExactive HF mass spectrometer was performed in a data dependent acquisition DDA mode of acquisition with a r 120,000 mz 200 full MS scan from mz 375 to 1600 with a target AGC value of 3e6 ions followed by 15 MSMS scans at r 30000 mz 200 at a target AGC value of 5e4 ions and 45 ms. A 20s dynamic exclusion was employed to increase depth of coverage. The total analysis cycle time for each sample injection was approximately 2 hours.\n\nFollowing 19 total LCMSMS analyses excluding conditioning runs, but including 4 replicate QC injections data was imported into Rosetta Elucidator v 4.0 Rosetta Biosoftware Inc., and analyses were aligned based on the accurate mass and retention time of detected ions features using PeakTeller algorithm in Elucidator. Relative peptide abundance was calculated based on area-under-the-curve AUC of the selected ion chromatograms of the aligned features across all runs. The MSMS data was searched against the SwissProt M. musculus database downloaded in May 2017 with additional proteins, including yeast ADH1, bovine serum albumin, as well as an equal number of reversed-sequence decoys false discovery rate determination. Mascot Distiller and Mascot Server v 2.5 Matrix Sciences were utilized to produce fragment ion spectra and to perform the database searches. Database search parameters included fixed modification on Cys carbamidomethyl and variable modifications on Meth oxidation and Asn and Gln deamidation. Full tryspin enzyme rules were used with up to 2 missed cleavages with mass tolerances of 5 ppm for precursor and 0.02da for product ions. After individual peptide scoring using the PeptideProphet algorithm in Elucidator, the data was annotated at a 1 percent peptide false discovery rate.\n","dates":{"publication":"Mon Aug 31 15:32:00 BST 2020"},"accession":"MSV000086045","cross_references":{}}