{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v03/MSV000086254/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"submitter":["Frank E. Loffler"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=b8f1f72f61c24e85abab272ba899a5a4"],"submitter_email":["frank.loeffler@utk.edu"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["68"],"ptm_modification":["UNIMOD:35 - \"Oxidation or Hydroxylation.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["LTQ XL"],"species":["Dehalococcoides Mccartyi (ncbitaxon:61435)"],"submitter_affiliation":["University of Tennessee-Knoxville"],"description_synonyms":["Water, Ethinyl Trichloride, Biological Markers, Viral Marker, Materials, Surrogate Endpoints, trichlorethylene, Laboratory, Subterranean Lakes, dhc64C, Biochemical, Endpoint, Trichloroethene, Serum, Ethinyl, Solvent, Underground Lake, trichloro-, Trichloride, Laboratory Markers, Groundwaters, Biological, BTBD34, Underground Waters, Fs(3)Laborc, dhc64c, vitamin, cDhc64C, 1, Dhc36c, Dehalococcoidetes, Dhc, DHC, Ground Water, CG7092, Eubacteria, ethene, Aethylen, Tables, CG3723, Waters, dhc, Bacteria Woese et al. 2024, CD, Immune, Markers, Bacteria <bacteria>, Prokaryotae, Viral Markers, TF2S, Procaryotae, Strains, acetylene trichloride, Trielina, B61, Underground Water, 1-dichloro-2-chloroethylene, Viral, Surrogate Endpoint, Underground Streams, Ethene, Dhc46C, Biochemical Markers, Fs(3)Sz18, Biologic Marker, DmelCG15804, trichlor, Ground Waters, Vinyl, trichloroethylene, Marker, SII, Genetic Materials, prokaryotes, triciene, Subterranean, DHC64C, Genetic Material, Vitamin, End Points, vitamines, Aethen, cDhc, Dhc64, DmelCG7092, INSDC_feature:gene, Immunologic, Laboratory Marker, ethinyl trichloride, Dehalococcoides ethenogenes, EDP1, Su(Gl)77, Material, Biochemical Marker, Underground, DmelCG3723, HMW MAP, Cistron, Trilene, H2C=CH2, Lakes, Strains and Sprains, vitaminum, Ground-Water, TCEA., Prokaryota, CH2=CH2, vitamine, Underground Stream, Clinical Markers, Clinical Marker, Water Table, Gene, l(3)64Ca, GTF2S, eubacteria, vitamina, CG1842, CG5526, Surrogate End Points, Surrogate Markers, Cdhc, R-1150, DmelCG7507, Carrying, Bacteriobiota, Dhc64c, Underground Lakes, Sprains, TFIIS, Biomarker, Ethylene, Ground-Waters, Genetic, Clinical, Aquifers, Biological Marker, chloro-, vitaminas, CG18613, Monera, Bacteria (ex Cavalier-Smith 1987), Table, Immunologic Markers, TCEA, Lab, biological marker, vitamins, fungi, Immunologic Marker, Biologic, Fs(3)Lab, CG15804, bacteria, Trichloroethylene, trichloraethylenum pro narcosi, Trichloraethylen, Lake, Serum Markers, Sprain, ethylene trichloride, CI-B12, End Point, Stream, Cistrons, Cell, CG7507, Immune Marker, DmelCG1842, Surrogate End Point, DmelCG5526, trichloroethylenum, Strain, Trichloraethen, TCE, Dynein, Biologic Markers, Narcogen, Aquifer, Serum Marker, Chloroethylene, Surrogate, not Bacteria Haeckel 1894, Endpoints, Chloride, Dhc 93AB, Surrogate Marker, FCP-A, Streams, hBACURD2, B12, prokaryote, Subterranean Lake, Water Tables, Immune Markers"],"name_synonyms":["TFIIS, Vinyl, CH2=CH2, Dehalococcoides ethenogenes., Ethylene, R-1150, Chloroethylene, TCEA, chloro-, ethene, Aethen, Ethene, Aethylen, SII, Chloride, TF2S, GTF2S, H2C=CH2"],"additional_accession":["PXD021905"]},"is_claimable":false,"name":"Respiratory vinyl chloride reductive dechlorination to ethene in TceA-expressing Dehalococcoides mccartyi","description":"Organohalide-respiring Dehalococcoidia bacteria are one of the few microorganisms capable of transforming chlorinated solvents to benign ethene in anoxic environments. The tceA gene found in these bacteria, coding the trichloroethene-dechlorinating RDase TceA, is frequently detected in contaminated groundwater but not recognized as a biomarker for vinyl chloride detoxification. Here, we demonstrate that the tceA-carrying Dehalococcoides mccartyi (Dhc) strains FL2 and 195 grow with VC as electron acceptor when sufficient vitamin B12 is provided. Global proteomic profiling confirmed the predominant TceA expression in VC-grown Dhc FL2 cells, providing a line of evidence for the implication of TceA in respiratory VC reductive dechlorination.","dates":{"publication":"Fri Oct 09 09:18:00 BST 2020"},"accession":"MSV000086254","cross_references":{}}