MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v03/MSV000086254/primaryOK200Frank E. Lofflerhttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=b8f1f72f61c24e85abab272ba899a5a4frank.loeffler@utk.eduMassIVE68UNIMOD:35 - "Oxidation or Hydroxylation."ProteomicsLTQ XLDehalococcoides Mccartyi (ncbitaxon:61435)University of Tennessee-KnoxvillePXD021905falseRespiratory vinyl chloride reductive dechlorination to ethene in TceA-expressing Dehalococcoides mccartyiOrganohalide-respiring Dehalococcoidia bacteria are one of the few microorganisms capable of transforming chlorinated solvents to benign ethene in anoxic environments. The tceA gene found in these bacteria, coding the trichloroethene-dechlorinating RDase TceA, is frequently detected in contaminated groundwater but not recognized as a biomarker for vinyl chloride detoxification. Here, we demonstrate that the tceA-carrying Dehalococcoides mccartyi (Dhc) strains FL2 and 195 grow with VC as electron acceptor when sufficient vitamin B12 is provided. Global proteomic profiling confirmed the predominant TceA expression in VC-grown Dhc FL2 cells, providing a line of evidence for the implication of TceA in respiratory VC reductive dechlorination.Fri Oct 09 09:18:00 BST 2020MSV000086254