MassIVE

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ftp://massive-ftp.ucsd.edu/v03/MSV000086294/primaryOK2001000Anthony Haaghttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=9e9343b12d3540e7bc8c470f2de719d3haag@bcm.eduMassIVE18UNIMOD:35 - "Oxidation or Hydroxylation."ProteomicsOrbitrap FusionBifidobacterium Dentium (ncbitaxon:1689)Baylor College of Medicine / Texas Children's HospitalBackground: Bacteroidetes are the most common bacterial phylum in the mammalian intestine and the effects of several Bacteroides spp. on multiple facets of host physiology have been previously described. Of the Bacteroides spp., Bacteroides ovatus has recently garnered attention due to its beneficial effects in the context of intestinal inflammation. In this study, we aimed to examine model host intestinal physiological conditions and dietary modifications to characterize their effects on B. ovatus growth. Methods and Results: Using Biolog phenotypic microarrays, we evaluated 62 primary carbon sources and determined that B. ovatus ATCC 8384 can use the following carbohydrates as primary carbon sources: 10 disaccharides, 4 trisaccharides, 4 polysaccharides, 4 polymers, 3 L-linked sugars, 6 D-linked sugars, 5 amino-sugars, 6 alcohol sugars, and 15 organic acids. Proteomic profiling of B. ovatus bacteria revealed that a significant portion of the B. ovatus proteome contains proteins important for metabolism. Among the proteins, we found glycosyl hydrolase (GH) familes GH2, GH5, GH20, GH 43, GH88, GH92, and GH95. We also identified multiple proteins with antioxidant properties and reasoned that these proteins may support B. ovatus growth in the GI tract. Upon further testing, we showed that B. ovatus grew robustly in various pH, osmolarity, bile, ethanol, and H2O2 concentrations; indicating that B. ovatus is a well-adapted gut microbe. Conclusion: Taken together, we have demonstrated that key host and diet-derived changes in the intestinal environment influence B. ovatus growth. These data provide the framework for future work toward understanding how diet and lifestyle interventions may promote a beneficial environment for B. ovatus growth.Unraveling the Metabolic Requirements of the Gut Commensal Bacteroides ovatus.Fultz Robert R, Ticer Taylor T, Ihekweazu Faith D FD, Horvath Thomas D TD, Haidacher Sigmund J SJ, Hoch Kathleen M KM, Bajaj Meghna M, Spinler Jennifer K JK, Haag Anthony M AM, Buffington Shelly A SA, Engevik Melinda A MAprotoplasm, Protein Gene Products, liquid chromatography tandem mass spectroscopy, LC-MS2, Gene Proteins, LC/MS/MS, nucleocytoplasm, LCMSMS, protoplast, liquid chromatography-tandem mass spectroscopy, Protein, LC-MSMS, Gene Products, Proteins, LC-MS/MS, liquid chromatography tandem mass spectrometry, LC-MS-MS., Gene, internal to cell, intracellularFood Patterns, Bowel, Ristella, Metabolic Concepts, growth and development, sci, glucide, carbohydrates, Carbon-12, DmelCG12298, Method, Lifestyle Factor, glucido, Osmolality, HOW, How, SCRAMBLED, Diet, Metabolism Concept, C, Grain, Osmolar, catabolism, organic acids, alimentary tract, stru, Intestine, metabolic process resulting in cell growth, procedures, l(3)S053606, Food, Social, Bacteria Woese et al. 2024, Restriction Diet Therapy, Dehydrated ethanol, g, Bacteria <bacteria>, H13, Prokaryotae, Concentrations, Glycan, Procaryotae, alimentary system, adult alimentary canal, spiritus vini, Disaccharide, metabolism resulting in cell growth, Antioxidants, STRUBBELIG, Endogenous, saccharides, Procedure, Glykane, Absolute Alcohol, Vitreous, Life Styles, DmIKKgamma, dIKK, numerical data, prokaryotes, secretion, Osmolarities, [OEtH], Digestive Tract, Kohlenhydrat, IKK-gamma, Methodological, carbohidratos, alcohol etilico, Methylcarbinol, carbohidrato, Dietary Patterns, gastrointestinal system, IMPAS, Innate, Dietary Restriction, GI Tract, Prokaryota, Sphingobacteria, Bacteroides-Cytophaga-Flexibacter group, Diet Therapies, macromolecules, Processes, Endogenous Antioxidant, carbohydrate, etanol, saccharide, antioxydant, enteric tract, Metabolic Processes, dIKK-gamma, Patterns, signal peptide peptidase activity, Trisaccharide, Gus, Gur, Gut, Diet Modification, DmIKK-gamma, Gene Products, Diets, Projections and Predictions, Technique, Polysaccharide, Lifestyle Factors, Modification, DmelCG6525, gut, Food Pattern, ethanol, mei-1794, Ionic Strengths, Metabolic Concept, inflammation, FBgn0082831, Study, Bacteria (ex Cavalier-Smith 1987), gastro-intestinal system, C2H5OH, Strengths, "Bacteroidota" Whitman et al. 2018, PSENL3, Innate Inflammatory Response, Restrictive Diet, Restriction Diet, Antioxidant Activity, dJ324O17.1, Osmolar Concentrations, Dietary Pattern, Ionic Strength, bacteria, Life Style Induced Illness, use, polymer molecule, degradation, Proteins, Spp, SPP, function, Factor, qkr, carbone, l(3)S090417, CFB group bacteria, Concept, carbono, gut tube, IKKgamma, Antioxidant Effect, KH93F, alcool ethylique, spp, Focus of Attention, glucides, metabolism, Lifestyles, Metabolic Phenomenon, polisacaridos, multicellular organism metabolic process, Bacteroidetes, Dmikkgamma, Dub, postnatal growth, Gus-u, Gus-t, Gus-s, Mental Concentration, Gus-r, Understanding, CG16910, Sugar, Gene Proteins, Osmolarity, Restrictive Diet Therapy, Polymer, prokaryote, CG6525, Restriction, Bacteroides fragilis subsp. ovatus, macromolecule, Anabolism, biochemical pathways, Anti Oxidants, host organism, Metabolic Process, Grain Alcohol, Inflammations, postnatal development., Activity, CFB group, postnatal development, Ristella <CFB group bacteria>, hydrates de carbone, Glykan, 1-hydroxyethane, alcohol, Restrictive, SUB, Techniques, polisacarido, Hydrolase, Ethanol, Readability, IMP1, Alkohol, T18E12.21, Concepts, Impacts, Environmental Impacts, Phenomenon, KIF20A, IKKg, l(3)j5D5, Cytophagales, KEY, Key, 24B, Anti Oxidant, Kohlenhydrate, Eubacteria, T18E12_21, inflammatory response, Glycans, GH-V, Ethyl, Glycane, Dietary Modifications, Futurology, Aethanol, Lifestyle, [CH2Me(OH)], CG10293, intestinal tract, Environmental Impact, l(3)j5B5, bowel, Methodological Studies, Antioxidant, Sludge, Carbon, Anti-Oxidant Effect, biotransformation, digestive tube, asd, Catabolism, Biliary Sludge, GI Tracts, Predictions and Projections, Therapy, 0904/17, Alcohol, Process, statistics and numerical data, PSL3, SPPL1, IMPAS-1, Attention Focus, Ethyl alcohol, Anti-Oxidants, Attention, SZ1, Capsularis, Kenny, ETHANOL, CFB Group Bacteria, saccharidum, polymer, gastrointestinal tract, antioxidants, AI747421, Digestive Tracts, EtOH, growth pattern, Cytophaga-Flavobacteria-Bacteroides Group Bacteria, non-developmental growth, 6C, Methodological Study, polymers, GI tract, Pseudobacterium ovatum, polysaccharides, Vitreous Carbon, Endogenous Antioxidants, DmelCG16910, MSTP086, carbonium, anon-EST:Liang-2.39, Pasteurella ovata, Gastrointestinal Tracts, a carbohydrate, glucidos, Proteomes, Dietary Restrictions, Strength, Mental, gastrointestinal (GI) tract, AT1G11140, Inflammatory Response, digestive canal, hGH-V, Procedures, Cytophaga-Flexibacter-Bacteroides phylum, P62, antoxidant, glycans, Gene, Bacteroidetes <Bacteroidota>, Selective, carbon, eubacteria, Dietary Modification, CG12298, Aethylalkohol, utilization, Metabolism, Anti-Oxidant, Studies, Bacteriobiota, dmIKKgamma, Anti-Oxidant Effects, IKK[[gamma]], Metabolism Phenomena, Ionic, ATSPP, Osmolalities, study, l(3)s2612, Absolute, gastroenterological system, Monera, Pattern, Kohlenstoff, Social Attention, Restriction Diet Therapies, IKK, Concentration, Biliary, DmelCG10293, SRF9, fungi, intestines, signal peptide peptidase, GHL, fel, clone 2.39, total expressed protein, GHV, Anti Oxidant Effect, Metabolic Phenomena, Impact, Carbohydrates, STRUBBELIG-RECEPTOR FAMILY 9, development, Environmental, Metabolism Concepts, Selective Attention, Protein, Phenomena, techniques, alimentary canal, who, Ethyl Alcohol, Anti Oxidant Effects, BCF group, biodegradation, Metabolic, Dietary, Restrictive Diet Therapies, Who/How, Carbohydrate, Diet Therapy, Carbon 12, Protein Gene Products, Antioxidant Effects, hydroxyethane, gall, Innate Inflammatory Responses, Diet Modifications, Environments, qkr[93F], Future, growth, T19D16.8, SCM, methodologydigestive canal, AI747421, gut, Bowel, Gus-u, alimentary tract, Gus-t, Gus-s, enteric tract, Gus-r, Bacteroides fragilis subsp. ovatus., Pseudobacterium ovatum, gut tube, Gus, Gur, Gut, g, gastrointestinal system, digestive tube, asd, Pasteurella ovata, adult alimentary canal, alimentary system, intestines, alimentary canalprotoplasm, Protein Gene Products, Gene Products, Proteins, Gene Proteins, Gene, nucleocytoplasm, internal to cell, protoplast, Protein., intracellular, Protein1PXD022012falseB. dentium intracellular proteinsB. dentium intracellular proteins identified via high-resolution LC-MS/MSThu Oct 15 11:33:00 BST 2020MSV00008629434899632