{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v03/MSV000086602/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Dr. Sebastian Wiese"],"instrument_platform":["LTQ Orbitrap Velos"],"species":["Homo Sapiens (ncbitaxon:9606)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=a0c624eba8c74781a3149eec107f6a48"],"submitter_email":["sebastian.wiese@uni-ulm.de"],"submitter_affiliation":["Ulm University"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["4"],"ptm_modification":["UNIMOD:4 - \"Iodoacetamide derivative.\""],"data_protocol":[""],"pubmed_abstract":["The respiratory tract is a major entry site for microbial pathogens. To combat bacterial infections, the immune system has various defense mechanisms at its disposal, including antimicrobial peptides (AMPs). To search for novel AMPs from the respiratory tract, a peptide library from human broncho-alveolar-lavage (BAL) fluid was screened for antimicrobial activity by radial diffusion assays allowing the efficient detection of antibacterial activity within a small sample size. After repeated testing-cycles and subsequent purification, we identified ß-2-microglobulin (B2M) in antibacterially active fractions. B2M belongs to the MHC-1 receptor complex present at the surface of nucleated cells. It is known to inhibit the growth of <i>Listeria monocytogenes</i> and <i>Escherichia coli</i> and to facilitate phagocytosis of <i>Staphylococcus aureus</i>. Using commercially available B2M we confirmed a dose-dependent inhibition of <i>Pseudomonas aeruginosa</i> and <i>L. monocytogenes</i>. To characterize AMP activity within the B2M sequence, peptide fragments of the molecule were tested for antimicrobial activity. Activity could be localized to the C-terminal part of B2M. Investigating pH dependency of the antimicrobial activity of B2M demonstrated an increased activity at pH values of 5.5 and below, a hallmark of infection and inflammation. Sytox green uptake into bacterial cells following the exposure to B2M was determined and revealed a pH-dependent loss of bacterial membrane integrity. TEM analysis showed areas of disrupted bacterial membranes in <i>L. monocytogenes</i> incubated with B2M and high amounts of lysed bacterial cells. In conclusion, B2M as part of a ubiquitous cell surface complex may represent a potent antimicrobial agent by interfering with bacterial membrane integrity."],"pubmed_title":["Respiratory ß-2-Microglobulin exerts pH dependent antimicrobial activity."],"pubmed_authors":["Holch Armin A, Bauer Richard R, Olari Lia-Raluca LR, Rodriguez Armando A AA, Ständker Ludger L, Preising Nico N, Karacan Merve M, Wiese Sebastian S, Walther Paul P, Ruiz-Blanco Yasser B YB, Sanchez-Garcia Elsa E, Schumann Christian C, Münch Jan J, Spellerberg Barbara B"],"description_synonyms":["Bronchioalveolar, Lung, PRSS, Bronchopulmonary, beta-Trypsin, Tripcellim, Lavages, Bronchoalveolar, Lung Lavages, Bronchoalveolar Lavages, sample population, Lung Lavage, Lavage, Trypure, Bronchioalveolar Lavage, Bronchial Lavage, CDABP0092, Bronchial Lavages, sample, Bronchopulmonary Lavage, Bronchial, Bronchioalveolar Lavages, HDCMA22P, Bronchopulmonary Lavages, beta Trypsin."],"name_synonyms":["anon-EST:fe1E6, prosbeta2, anon-EST:fe1G6, beta 2, Bronchioalveolar, Lung, human being, l(3)DTS7, Man (Taxonomy), DTS-7, Bronchioalveolar., Bronchopulmonary, Modern, beta2_dm, Beta-2, b2, beta2, Lavages, Bronchoalveolar, Lung Lavages, human, Bronchoalveolar Lavages, CG3329, Human, Lung Lavage, Lavage, Prosbeta, Bronchioalveolar Lavage, Homo sapiens, DmelCG3329, Bronchial Lavage, Modern Man, Bronchial Lavages, 3329, DTS7, Prosbeta 2, Dts7, Bronchopulmonary Lavage, Bronchial, Bronchioalveolar Lavages, Bronchopulmonary Lavages, Man, humans"],"pubmed_abstract_synonyms":["mixed phenotype acute leukaemia, Bile salt-stimulated lipase, Size, Ifm(2)RU1, Dm II, dAmph, APRTD, antimicrobials, Adenylic Acid, Synthetic Peptide, antimicrobial agents, bacterium E3, growth and development, Anti-Infective Agent, 5'-Adenosine monophosphate, fluid, cell associated, QA1, Myo, Polypeptides, microbicides, damph, S-7020, Anti Infective Agent, ifm(2)2, ampicillin acid, AP, Adenosine-5'-monophosphoric acid, 1, 2, aminobenzylpenicillin, integumentum commune, antimicrobial, 3-hydroxy-1, ampicillin anhydrous, molecules, Escherichia/Shigella coli, Myo2, Bricd4, Antibiotika, Solute carrier family 27 member 5, Tissue, DROMHC, Anti-Microbial, Micrococcus aureus, Molekuel, Synthetic Peptide Library, Diffusions, adenosine phosphate, 5'-Adenylic acid, Respiratory Systems, isolation and purification, NMMII, Eschericia coli, ifm(2)RU2, Bacterium monocytogenes, ampicilina, HLA-6.2, dithioglycerol, 1110017I01Rik, EA1.2, Infections and Infestations, tracheal system, Myo II, Very long-chain acyl-CoA synthetase-related protein, sMHC, Douchings, dimercaptopropanol, Adl, 2'-Adenosine Monophosphate, external covering of organism, (2S, Adenosine Phosphate Dipotassium, Antiinfective, Respiratory system, integral to membrane, ifm(2)RU1, Enteroaggregative E. coli, 2'-Adenylic Acid, Sht, Diffusely Adherent E. coli, Carboxyl ester lipase, Sizes, 3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid, EA2.1, Phagocytoses, D-(-)-6-(alpha-aminophenylacetamido)penicillanic acid, Acid, Enteroinvasive E. coli, Bacterial Diseases, Adenosine 5'-Phosphate, Infection and Infestation, alpha, Lavages, human, Lavage, Adenosine, Anti-Microbial Agent, BACS, spindle cells, AML with lymphoid markers, MyoII, Bacillus aeruginosus, Monophosphate, Innate, humans, DmelCG8604, Bacterial Disease, localised, antibiotique, Peptide Library, Human, Agent, surface, British antilewisite, Dm nmII, isolation, Phage Display Library, 3-dimercaptopropanol, Escherchia coli, Sample Sizes, Random Peptide Library, AW214463, 2-propanedithiol, 5'-Adenylic, Man, Irrigation, Synthetic, dimercaprol, Therapeutic Irrigations, Myo-II, Erysipelothrix monocytogenes, Tissues, zip/MyoII, mixed phenotype acute leukemia, inflammation, Corynebacterium parvulum, clinical infection, Innate Inflammatory Response, 2-dithioglycerol, beta2m, Defense, probable synonym or variety: \"Pseudomonas polycolor\" Clara 1930, peptidos, BA-CoA ligase, 5'-Phosphate, Respiratory Tract, DmnmII, Staphylococus aureus, Antibiotikum, Alkalescens-Dispar Group, 2'-AMP, Listerella hepatolytica, MFT1, Random, VLACS-related, E(br), EAggEC, Libraries, Bagl, Cell, AMP, Amp, nmMHC, British anti-Lewisite, chemical analysis, 5'-Adenylic Acid, Synthetic Peptide Libraries, British anti-lewisite, CG8604, Mhc-c, Adenosine Phosphate, Apparatus respiratorius, respiratory tract, Library, Peptide Fragment, Synthetic Peptide Combinatorial Library, Adenosine 5'-phosphate, Bacterial, 6R)-6-{[(2R)-2-amino-2-phenylethanoyl]amino}-3, Anti Microbial Agent, Staphylococcus aureus subsp. anaerobius, underdeveloped, ABPC, increased number, adenosine-5'P, postnatal growth, pA, CDMT, Adenosine 2'-Phosphate, MFT, primary structure of sequence macromolecule, Tract, BALL, PARP-9, Dipotassium, Micrococcus pyogenes, Bacterium coli commune, Bacterium aeruginosum, Antimicrobial Agent, dithioglycerine, Fatty acid transport protein 5, 2' Adenylic Acid, S 7020, General activity, Adenylic acid, Adenosine 5' Phosphate, sulfactin, Immune Systems, Inflammations, Activity, MHC, determination, Stp, postnatal development, Infestations and Infections, Bsh, Corynebacterium infantisepticum, neutral molecular compounds, PAdo, FCAALL.51, BRICD4, Mechanisms, Pancreatic lysophospholipase, 6-(D-(2-amino-2-phenylacetamido))-3, Membrane Tissues, peptido, Fragments, mhc, Cholate--CoA ligase, 3-mercaptopropan-1-ol, Enterococcus coli, Staphlococcus pyogenes citreus, molecule, FBgn0002741, molecula, CG17927, ARTD9, CYLDI, increased, 3-DIMETHYL-7-OXO-4-THIA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC ACID, Man (Taxonomy), inflammatory response, peptides, mMHC, Staphilococcus aureus, l(2)M66, Random Peptide Libraries, membrane region, 2-dimercapto-3-propanol, hypoplasia, Microbicides, E coli, subdivision of respiratory tract, purification, ASL, CYLD1, Bacillus pyocyaneus, E. coli, phosphate d'adenosine, Enteroaggregative Escherichia coli, Immune, Micrococcus pyocyaneus, Respiratory, acute leukaemia of undetermined lineage, NMM, BSSL, l(2)k10423, Irrigations, Mhc36B, l(2)36Ae, antibiotic, Anhydrous ampicillin, dimercaprolum, Membrane Tissue, Adenosine 3'-Phosphate, USPL2, Modern, Mhc-c[1], Bssl, organism surface, Bacterium monocytogenes hominis, membranous organ component, myoII, Douching, Adenosine 3' Phosphate, 3-dimethyl-7-oxo-4-thia-1-azabicyclo(3.2.0)heptane-2-carboxylic acid, beta2-m, Lysteria monocytogenes, ALL with myeloid markers, 2'-Adenylic, BRSS, fosfato de adenosina, Anti Infective Agents, microbicide, 3-Mercaptopropanol, VLACSR, HDCMA22P, region, 5R, Peptide Libraries, Diffusely Adherent Escherichia coli, Cholesterol esterase, membrane of organ, growth pattern, non-developmental growth, System, DL4475C, Phosphaden, Peptide Phage Display Library, 6R)-6-{[(2R)-2-AMINO-2-PHENYLETHANOYL]AMINO}-3, Anti Microbial Agents, 5'-O-phosphonoadenosine, Random Peptide, ampicillinum, loss of, Respiratory Tracts, Pseudomonas polycolor, Sample, Staphylococcus pyogenes aureus, Agents, acute leukemia of undetermined lineage, integumentary system, Polypeptide, 3-Dimercapto-1-propanol, Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980), dermal system, cell bound, adenosini phosphas, accessory, DAMP, Zip, ZIP, Inflammatory Response, human being, Sterol esterase, amph, Phosphate Disodium, Fragment, Pseudomonas pyocyanea, CHM1L, Adenosine 2' Phosphate, Bacterial Infections, Myo-II HC, Bacterium pyocyaneum, adenosine 5'-(dihydrogen phosphate), supernumerary, Damp, 3-dithiopropanol, EAC, Homo sapiens, reduced, CONSTITUTIVE 1, CDABP0092, integral component of membrane, Anti-Infective, AMPS, SUPPRESSOR OF NPR1-1, Enteroinvasive Escherichia coli, UNQ771/PRO1565, tiny, 6R)-6-{[(2R)-2-amino-2-phenylacetyl]amino}-3, antibiotics, CG15792, 3-dimercaptol-1-propanol, Ly-m11, 5'-AMP, ChM1L, Bacterial Infection, 2' Adenosine Monophosphate, Adenylate, localized, Infections, MRP, Mrp, Ifm(2)2, Peudomonas aeruginosa, Anti-Microbial Agents, DmMHC, rBAL-1, ASASE, DmelCG17927, Infestation and Infection, ampicilline, Nup, Ado5'P, Antiinfective Agents, region of membrane, FATP-5, site, Microbicide, l(2)02957, small, Antimicrobial, ADENOSINE MONOPHOSPHATE, membrane, Bacterium coli, DroMII, Atmungssystem, beta-dithioglycerol, biphenotypic acute leukemia, 5' Adenylic Acid, apparatus respiratorius, 2'-Adenosine, Peptide, 1810036E18Rik, development, 6.2.1.7, ampicillin, membrane tetraspanin-enriched microdomain, uptake, Bacillus coli, Systems, Infection, whole membrane, sequence, 3-dimercapto-1-propanol, D-(-)-ampicillin, Membrane., 3.1.1.13, DmelCG15792, Bile acid-CoA ligase, transmembrane, Phosphate Dipotassium, body surface, biphenotypic acute leukaemia, Disodium, Membrane, 3.1.1.3, Phage Display Peptide Library, TeM, Streptococcus aureus, present in greater numbers in organism, Antimicrobial Agents, MyHC, Adenosine Phosphate Disodium, SBS, Therapeutic, Bal, Innate Inflammatory Responses, Modern Man, BAL, BC003281, Dronm-MII, anon-WO0140519.37, Peptid, assay, growth, zpr, TEM, nmy-2"],"pubmed_title_synonyms":["antibiotic, antimicrobial, General activity., microbicide, antimicrobial agents, microbicides, Activity, antibiotique, antimicrobials, antibiotics, Antibiotika, Antibiotikum"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"Identification of human beta-2 microglobulin in bronchoalveolar lavage","description":"The uploaded data is related to the identification of beta-2-microglobulin isolated from bronchoalveolar lavage. The sample was digested with trypsin and the proteolytic fragments were identified by MS/MS","dates":{"publication":"Tue Dec 15 15:20:00 GMT 2020"},"accession":"MSV000086602","cross_references":{"pubmed":["33092477"]}}