MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v03/MSV000087150/primaryOK2000000ProteomicsLeonard Fosterimpact IIApis Mellifera (ncbitaxon:7460)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=c2dd464b5137446b94d9676ac2995b98foster@msl.ubc.caUniversity of British ColumbiaMassIVE7Oxidation [M]Carbamidomethylation [C]Acetylation [Protein N-term]l(2)k04204, Ammonium, OG12X, Peptidomics, Gene, Carbamide, 6330543G17Rik, Basodexan, [NH4](+), gonada of female organism genitalia, Buffer, l(2)k05821, Karbamid, ammonium cation, Polypeptides, gonad of genitalia of female organism, peptido, l(2)k15606, reduced, azanium, Harnstoff, Gene Products, tiny, Carmol, Shot, genitalia of female organism gonada, Apis mellifica, gonada of female organism reproductive system, l(2)k10821, peptides, genitalia of female organism gonad, E927b, ammonium ion, ovum-producing ovary, beta-Trypsin, Su(Mir)1, hypoplasia, female organism reproductive system gonad, reproductive system of female organism gonada, reproductive system of female organism gonad, sample, honey bee, ovary - animal, ovary, peptidos, l(2)CA4, female organism genitalia gonad, Prx3, female reproductive system gonada, Ammonium(1+), small, bee, gonad of female reproductive system, kop, gonada of reproductive system of female organism, gonad of female organism reproductive system, gonada of female reproductive system, Trypsin/K, Proteins, Liquid Chromatography, CG18076, NH4+, CG18637, buffer, ur, Peptide, shortstop/kakapo, carbamide, NH4(+), carbonyldiamide, Ovaries, honeybee, DmelCG18076, Protein, female reproductive system gonad, l(2)k05434, ovaries, female organism reproductive system gonada, animal ovary, PRSS, ammonium, Grv, gonada of genitalia of female organism, underdeveloped, uree, l(2)k03010, female gonad, ovarium, gonad of reproductive system of female organism, Tripcellim, kak, gonad of female organism genitalia, beta Trypsin, SHOT, OG12, sample population, Protein Gene Products, Gene Proteins, Trypure, H2NC(O)NH2, female organism genitalia gonada, grv, Peptid, Polypeptide, Og12x, anatomical systems., 42/4, 1728Apis mellifica, bee, ovaries, female organism reproductive system gonada, gonada of female organism reproductive system, animal ovary, gonad of female reproductive system, gonada of genitalia of female organism, Peptidomics, gonada of reproductive system of female organism, gonad of female organism reproductive system, genitalia of female organism gonad, gonada of female reproductive system, ovum-producing ovary, female gonad, ovarium, gonad of reproductive system of female organism, female organism reproductive system gonad, gonad of female organism genitalia, gonada of female organism genitalia, gonad of genitalia of female organism, reproductive system of female organism gonada, female organism genitalia gonada, reproductive system of female organism gonad, honeybee., Ovaries, ovary - animal, ovary, honey bee, female organism genitalia gonad, female reproductive system gonad, female reproductive system gonada, genitalia of female organism gonada0falseProteomics on the ovaries of failed and healthy honey bee queensWe sampled honey bee queens from beekeepers throughout British Columbia which were rated as either 'failed' or 'healthy' by beekeepers. We performed shot-gun proteomics on the ovaries. Proteins were reduced, alkylated, and digested with Lys-C for 4 h in urea digestion buffer. The sample was then diluted 4x with ammonium bicarbonated and digested overnight with trypsin. Peptides were analyzed by an EASY-nLC 1000 liquid chromatography system coupled to a Bruker Impact II mass spectrometer. Data were searched with MaxQuant and the zipped txt folder output is provided.Fri Apr 02 09:37:00 BST 2021MSV000087150