{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v03/MSV000087325/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Jianping Wu"],"instrument_platform":["Orbitrap Fusion Lumos"],"species":["Mus Musculus (ncbitaxon:10090)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=8a1086884d424c2b854e45e113124595"],"submitter_email":["wujianping@westlake.edu.cn"],"submitter_affiliation":["Westlake University"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["5"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"pubmed_abstract":["The cation channel of sperm (CatSper) is essential for sperm motility and fertility<sup>1,2</sup>. CatSper comprises the pore-forming proteins CATSPER1-4 and multiple auxiliary subunits, including CATSPERβ, γ, δ, ε, ζ, and EFCAB9<sup>1,3-9</sup>. Here we report the cryo-electron microscopy (cryo-EM) structure of the CatSper complex isolated from mouse sperm. In the extracellular view, CATSPER1-4 conform to the conventional domain-swapped voltage-gated ion channel fold<sup>10</sup>, following a counterclockwise arrangement. The auxiliary subunits CATSPERβ, γ, δ and ε-each of which contains a single transmembrane segment and a large extracellular domain-constitute a pavilion-like structure that stabilizes the entire complex through interactions with CATSPER4, 1, 3 and 2, respectively. Our EM map reveals several previously uncharacterized components, exemplified by the organic anion transporter SLCO6C1. We name this channel-transporter ultracomplex the CatSpermasome. The assembly and organization details of the CatSpermasome presented here lay the foundation for the development of CatSpermasome-related treatments for male infertility and non-hormonal contraceptives."],"pubmed_title":["Structure of a mammalian sperm cation channel complex."],"pubmed_authors":["Lin Shiyi S, Ke Meng M, Zhang Yuqi Y, Yan Zhen Z, Wu Jianping J"],"pubmed_abstract_synonyms":["Cryomicroscopies, Kation, Male, methionine aminopeptidase activity, Laboratory, Ass-1, FON1, cation, Mus domesticus, postnatal development, Mbp1, Non-Governmental Organizations, Organic Anion Transport Polypeptide, growth and development, Progress Reports, FLORAL ORGAN NUMBER 1, Foundation, House Mouse, World Fertility Survey, Fertility Preferences, serial element, Below Replacement, Summary Report, Ionic Channels, AA408052, SUP, fold, 2, Infertility, myd, Effect, Summary Reports, Fertility Surveys, peptidase M activity, Transporter, male, Transport Proteins, Male Infertility, X-Chromosome-Bearing Sperm, adenomas, sperm cell, Fertility Incentives, rabGAPLP, X-Bearing, L-methionine aminopeptidase activity, pore, Progress Report, Natural, membrane region, Bdr, CatSper channel complex, Mbp-1, RabGAP-5, Swiss Mice, Super protein, sp, Sub-Fertility, SNAP-25, ASS, Progress, Y Chromosome Bearing Sperm, Field Reports, \"male infertility\" EXACT [SNOMEDCT_2005_07_31:155924001], Y-Bearing Sperm, X Chromosome Bearing Sperm, RUSC3, infertility, Y-Chromosome-Bearing Sperms, Ionic Channel, Organic Anion Transport Protein, Fertility Determinant, Therapies, Fertility Preference, CatSper1, Cryoelectron, OATP Proteins, house mouse, Membrane Transport Proteins, Organizations, Differential Fertility, ejaculate, Therapy, Y-Chromosome-Bearing, Motility, sterility, OATP Transporters, organisation, gyltl1b-b, World Fertility Surveys, mouse, MYH-associated polyposis, autosomal recessive familial adenomatous polyposis, Contraceptives, Cryo-electron, integral to membrane, Maps, OAT Transport Protein, segment, Organic Anion, male reproductive system infertility disorder, Investigative Report, Cryomicroscopy, MDDGA6, mKIAA0609, Electron Cryomicroscopy, GENA70, Cryoelectron Microscopies, Anion Transporter, KIAA0609, autosomal recessive, fg, Organic Anion Transport Proteins, Mus musculus, X-Chromosome-Bearing, RUTBC3, OAT, gyltl1b, sperm movement, Nongovernmental, mice, Channels, administrative structure, Field, Swiss Mouse, mdc1d, expanded, Membrane Channels, Electron, infertility disorder of male reproductive system, spermatozoid, X Bearing Sperm, Preferences, Treatments, Fertility Determinants, domesticus, RABGAP5, seminal fluid, MDC1D, Report, enr, \"Infertility, Agents, enlarged, unspecified\" EXACT [ICD9CM_2006:606.9], Sperms, segment - adult, multiple colorectal, Surveys, hCatSper, Microscopy, Mouse, Male Sub-Fertility, Fecundability, sperm flagellum movement involved in flagellated sperm movement, \"male infertility NOS (finding)\" EXACT [SNOMEDCT_2005_07_31:198018002], Marital Fertility, FLORAL DEFECTIVE 10, big, Subfecundity, Sperm, 4933404A18Rik, conformation, Sperm Motilities, Effects, SPGF7, Male Sterility, Gene, organization and administration, Fertility, SUPERMAN, Y-Chromosome-Bearing Sperm, froggy, Cryo-electron Microscopy, Gyltl1a, sperm motility, Subfertility, large, Determinant, Investigative, House, integral component of membrane, 1700007I06Rik, \"male infertility (finding)\" EXACT [SNOMEDCT_2005_07_31:2904007], Spermatozoon, Gene Products, Mus musculus domesticus, Survey, Non-Governmental, Preference, World Fertility, C79179, Y Bearing Sperm, Mice, Ionic, \"male infertility, familial adenomatous polyposis, Fecundity, MAP, Organization, Ion Channel, Swiss, MDDGB6, familial adenomatous polyposis 2, LARGE, X-Bearing Sperms, X-Chromosome-Bearing Sperms, autosomal recessive multiple colorectal adenomas, cations, BPFD#36, Mif1, Organic Anion Transporter, Male Subfertility, Non-Governmental Organization, sperm, Below Replacement Fertility, great, male infertility, flagellated sperm movement, region of membrane, Cation, CATSPER, SPERM, Fertility Incentive, Membrane Channel, organization, Investigative Reports, relational structural quality, Contraceptive, Contraceptive Effects, X-Bearing Sperm, MUTYH-Associated Polyposis, Microscopies, Cryo electron Microscopy, organizational structure, World, Sub Fertility, sperm flagellum movement, 4930403C08Rik, Proteins, Kationen, Synaptosomal-associated 25 kDa protein, Transporters, Organic Anion Transport Polypeptides, cationes, spermatozoon, development, Organic, Channel, male\" EXACT [ICD9CM_2006:606], Mus, Protein, Research Reports, whole membrane, Natural Fertility, Determinants, Nongovernmental Organizations, Y-Bearing, administrative management, transmembrane, Transport Protein, male sterility, Electron Cryomicroscopies, CATSPER channel, postnatal growth, Motilities, House Mice, OATP Transporter, FLO10, pore-forming toxin activity, Membrane, MYH-Associated Polyposis, HERP, OAT Transport, OAT Transport Proteins, Contraceptive Effect., Laboratory Mice, Protein Gene Products, Gene Proteins, extracellular, Y-Bearing Sperms, Differential, Non Governmental Organizations, Marital, Reports, Ion, Therapeutic, OATP, SNAP, male reproductive system infertility, Sterility, organizational management, MAP syndrome, Anion Transporters, Treatment, sperm flagellum movement involved in flagellated sperm motility, Cryo-electron Microscopies, Summary, Nongovernmental Organization, growth, Laboratory Mouse, Field Report, FAP2, Fertility Survey, channel-forming toxin activity, colorectal adenomatous polyposis"],"description_synonyms":["CATSPER channel, Industrial, CatSper channel complex., Industrial Arts, Arts"],"pubmed_title_synonyms":["X-Chromosome-Bearing Sperm, sperm cell, Y-Chromosome-Bearing, X-Chromosome-Bearing, X-Bearing Sperm, Sperm, X-Bearing, conformation, ejaculate., X-Bearing Sperms, Y-Chromosome-Bearing Sperm, spermatozoid, X Bearing Sperm, X-Chromosome-Bearing Sperms, spermatozoon, seminal fluid, Y Chromosome Bearing Sperm, Y-Bearing Sperms, Y-Bearing Sperm, sperm, X Chromosome Bearing Sperm, Sperms, Y-Chromosome-Bearing Sperms, Spermatozoon, SPERM, relational structural quality, Y-Bearing, Y Bearing Sperm"],"name_synonyms":["Mus musculus, Laboratory Mice., Laboratory, protein complex, Swiss, mice, Mus domesticus, Proteins, mouse, Swiss Mouse, Gene, House Mice, Swiss Mice, proteins, protein, protein-containing complex, House Mouse, domesticus, Protein Gene Products, Gene Proteins, protein polypeptide chains, native protein, natural protein, polypeptide chain, House, Mus, Protein, Gene Products, Mus musculus domesticus, Mouse, house mouse, protein aggregate, Mice, Laboratory Mouse"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"Protein ID of the purified mouse CatSpermasome","description":"Proteomic technology was used to identify the components of CatSper complex.","dates":{"publication":"Wed Apr 28 04:46:00 BST 2021"},"accession":"MSV000087325","cross_references":{"pubmed":["34225353"]}}