{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v03/MSV000087703/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Erika Ponzini"],"instrument_platform":["Orbitrap Fusion"],"species":["Homo Sapiens (ncbitaxon:9606)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=ea4ccb67729d41999b5d3088681effcc"],"submitter_email":["erika.ponzini@unimib.it"],"submitter_affiliation":["University of Milano-Bicocca"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["73"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"pubmed_abstract":["Lacrimal fluid is an attractive source of noninvasive biomarkers, the main limitation being the small sample amounts typically collected. Advanced analytical methods to allow for proteomics profiling from a few microliters are needed to develop innovative biomarkers, with attractive perspectives of applications to precision medicine. This work describes an effective, analytical pipeline for single-tear analysis by ultrahigh-resolution, shotgun proteomics from 23 healthy human volunteers, leading to high-confidence identification of a total of 890 proteins. Highly reproducible quantification was achieved by either peak intensity, peak area, or spectral counting. Hierarchical clustering revealed a stratification of females vs. males that did not emerge from previous studies on pooled samples. Two subjects were monitored weekly over 3 weeks. The samples clustered by withdrawal time of day (morning vs. afternoon) but not by follow-up week, with elevated levels of components of the immune system in the morning samples. This study demonstrates feasibility of single-tear quantitative proteomics, envisaging contributions of this unconventional body fluid to individualized approaches in biomedicine."],"pubmed_title":["Single-Tear Proteomics: A Feasible Approach to Precision Medicine."],"pubmed_authors":["Ponzini Erika E, Ami Diletta D, Duse Alessandro A, Santambrogio Carlo C, De Palma Antonella A, Di Silvestre Dario D, Mauri Pierluigi P, Pezzoli Fabio F, Natalello Antonino A, Tavazzi Silvia S, Grandori Rita R"],"description_synonyms":["D030046N04Rik, Methane, liquid chromatography tandem mass spectroscopy, IPP2A2, visual_system, FASN2C, ion, Meibum, leg, Untrained, heavy chain disease, trichloro-, l(1)AA33, 5730420M11Rik, Polypeptides, Techniques, peptido, Method, eye, 1, fabD, 2, globe, wood alcohol, SET, Divorced, LCMSMS, peptides, TAF-I, Personnel, eyes, visual system, Untrained Personnel, Divorces, analyzer, MCT1, SURGICAL AND MEDICAL PROCEDURES, DmelCG4299, IGAAD, set, Voluntary Worker, Komplexauge, Methodological Studies, l(1)B2/13.1, DmelCG10574, RUN, Run, MCT1D, sample, 5430437K10Rik, Sodium Methoxide, Volunteer Personnel, LC-MS2, phapii, eye globe, l(1)LB9, Alcohol, LC-MS/MS, Liquid Chromatography, Volunteerism, NET62, StF-IT-1, vertebrate eye, Procedure, AA33, Eyes, Tear, HCD, Ionen, Voluntary, Lipid, zusammengesetztes Auge, Carbinol, eyeball, carbinol, parent ion, HLA-DR-associated protein II, DI-2, I-2Dm, Orbital part of face, Methodological, CG4299, study protocol, Methodological Study, beta Trypsin, Trichloromethane, I-2PP1, Wood, TAF-IBETA, precursor ion, orbital part of face, spirit of wood, liquid chromatography-tandem mass spectroscopy, TAF-Ibeta, Polypeptide, i2pp2a, Methyl Alcohol, CG1849, tear fluid, compound eye, Procedures, regio orbitalis, Meibomian Lipid, adult compound eye, Gene, Methyl, CH3OH, precursor, Methylalkohol, PHAPII, LC-MS-MS, Intervention or Procedure, Volunteer Workers, Meibomian Lipids, method, camera-type eye plus associated structures, method used in an experiment, LC-MSMS, Studies, Gene Products, Wood Alcohol, Separated, Technique, study, Mitochondrial malonyl CoA - ACP acyltransferase, light-detecting organ, Orbital region, interventionDescription, iones, Volunteer Worker, Runt, ipp2a2, beta-Trypsin, Interventional, 2pp2a, Worker, ions, CG10574, Solution, Study, 2PP2A, wood naphtha, taf-ibeta, MCC, dSET, dSet, Voluntary Workers, peptidos, T mast cells, MC(T), [Acyl-carrier-protein] malonyltransferase, LB5, MCT, Intervention Strategies, HHF7, Proteins, DmelCG1849, igaad, bulbus oculi, Methyl alcohol, Rnt, Peptide, visual apparatus, group, lLB5, 2.3.1.39, LC/MS/MS, MT, Facettenauge, I-2PP2A, Protein, Dm I-2, I2PP2A, Sodium, MeOH, Mitochondrial malonyltransferase, count., Intervention, Separation, Volunteer, PRSS, ensemble, Separations, Tripcellim, l(1)19Ea, sample population, BRIT1, Protein Gene Products, plan specification, P235, Gene Proteins, Trypure, Meibomian, dSET/TAF-Ibeta, 2610030F17Rik, Pressures, Ion, orbital region, wood spirit, liquid chromatography tandem mass spectrometry, Peptid, AA407739, Methoxide"],"pubmed_title_synonyms":["Meibomian, Meibomian Lipids, Tear, tear fluid, Individualized, P-Health, Peptidomics, Predictive Medicine, Personalized Medicine, Meibum, Meibomian Lipid, Individualized Medicine, Lipid, Medicine, P Health, Precision, Theranostic, Predictive, Personalized, Theranostics."],"name_synonyms":["Human, Meibomian, Meibomian Lipids, Tear, tear fluid, human being, Man (Taxonomy), Homo sapiens, Meibum, Meibomian Lipid, Modern Man, Lipid, proteomic analysis, Modern, humans., Man, human"],"pubmed_abstract_synonyms":["Healthy Volunteer, Biological Markers, Viral Marker, tear fluid, Surrogate Endpoints, Immune Systems, Procedures, determination, Clinical Markers, Laboratory, Peptidomics, Participants, Effects, Individualized Medicine, Meibum, Meibomian Lipid, Clinical Marker, Biochemical, Endpoint, Gene, Human Volunteers, Serum, fluid, Long Term, Surrogate End Points, Human, Surrogate Markers, Laboratory Markers, Techniques, Meibomian Lipids, Healthy Subjects, reduced, Biological, Method, Normal Volunteers, Studies, Gene Products, body fluid, Participant, Precision, tiny, Effect, Technique, Biomarker, study, Clinical, Individualized, Longterm, Biological Marker, hypoplasia, Normal Volunteer, procedures, Long-Term, Human Volunteer, Study, Immunologic Markers, Immune, Markers, Methodological Studies, Healthy Participants, Viral Markers, Normal, sample, Medicine, Long-Term Effect, Predictive, Immunologic Marker, Body., Long-Term Effects, Biologic, small, Viral, Surrogate Endpoint, Predictive Medicine, Males, Serum Markers, Proteins, Longterm Effect, Fluids, End Point, Biochemical Markers, Procedure, Personalized, Biologic Marker, Subjects, Volunteers, Immune Marker, Tear, Fluid, Marker, Surrogate End Point, Personalized Medicine, Lipid, chemical analysis, Protein, Long Term Effects, Systems, techniques, Biologic Markers, Healthy Participant, Serum Marker, Volunteer, Body Fluid, End Points, underdeveloped, Surrogate, Healthy Subject, System, Endpoints, Theranostic, Immunologic, Methodological, Laboratory Marker, Methodological Study, Surrogate Marker, sample population, Body, Healthy, Longterm Effects, Protein Gene Products, Gene Proteins, Meibomian, P-Health, Biochemical Marker, Theranostics, Subject, P Health, assay, Females, methodology, Immune Markers"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic analysis of single tears from healthy human subjects","description":"23 volunteers were recruited for this study. Tears were collected using microcapillary tubes (MCT). For each volunteer, a single tear sample was taken from one eye. For two of these volunteers, the procedure was performed in the morning (9-10 AM) and in the afternoon (5-6 PM) on the same day, once a week for 3 consecutive weeks. Proteins were purified from 5 uL of tear sample using the methanol-chloroform precipitation protocol. The lyophilized proteins were resuspended in ammonium bicarbonate 50 mM, pH 8. An in-solution trypsin digestion was performed. For each run, 1 ug of peptides was injected and each sample was injected twice. Analyses were performed by LC-MS/MS using an Orbitrap Fusion mass spectrometer coupled to an EASY-nLC 1000 nanoflow high-pressure liquid chromatography. Peptides were separated on a commercial analytical nanocolumn. MS scans were performed using the Orbitrap (OT) analyzer with a resolution of 120 000. Precursor ions were selected for higher collisional dissociation (HCD) and the collision energy was set to 30%. Dynamic exclusion was set at 45 s, with a repeat count of 1.","dates":{"publication":"Fri Jun 25 08:03:00 BST 2021"},"accession":"MSV000087703","cross_references":{"pubmed":["34639092"]}}