{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v03/MSV000088030/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Ilma Rita Korponay-Szabo and Robert Kiraly"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=373b6d82927143c39460f09e290a58b9"],"submitter_email":["kiralyr@med.unideb.hu"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["63"],"ptm_modification":["UNIMOD:800 - \"Was PentylamineBiotin.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["Orbitrap Fusion"],"species":["Homo Sapiens (ncbitaxon:9606)"],"submitter_affiliation":["University of Debrecen"],"description_synonyms":["liquid chromatography tandem mass spectroscopy, 293 cell, AI747449, 4-d]imidazol-4-yl)pentanoic acid, human being, Deacura, Procedures, vitamin B7, Il-3, Biotine, Sepharose C1 4B, Str, Medebiotin, transglutaminase iv, Gene, CATX15, protein, Vitamin H, H105E3, protein-containing complex, Csfmu, prevention, LC-MS-MS, Human, 2-diyl)diphenol, 2-Bis(4-Hydroxyphenyl)propane, Polypeptides, method, Techniques, protein polypeptide chains, transglutaminase TGM4, peptido, Homo sapiens, polypeptide chain, Biotin, Method, Dystonia musculorum protein, method used in an experiment, LC-MSMS, 4beta, Studies, Line, Gene Products, 2, 4'-(1-Methylethylidene)bisphenol, 6aR)-Hexahydro-2-oxo-1H-thieno[3, 4, D6S1101, HEK 293, Human Embryonic Kidney 293, RCB1637, protein aggregate, 6aalpha))-, Gelfert, 2-Di(4-hydroxyphenyl)propane, prevention and control, Man, Technique, HCGF, 4'-(1-Methylethane-1, Microtubule actin cross-linking factor 2, MACF2, Sepharose, Biotin Hermes, 230 kDa bullous pemphigoid antigen, 1-diyl)diphenol, LCMSMS, Man (Taxonomy), peptides, reference sample, Coenzyme R, (3aS, cis-Hexahydro-2-oxo-1H-thieno(3, biotina, biotine, proteins, man, 4'-Isopropylidenediphenol, 2-Bis(4-hydroxyphenyl)propane, Study, Hemidesmosomal plaque protein, preventive measures, 4-d]imidazole-4-valeric acid, C1 4B, Hermes, Methodological Studies, (+)-cis-Hexahydro-2-oxo-1H-thieno[3, Agarose, bisphenol-A, peptidos, biotinum, BPAG1, 6-An-alpha-L-Galp-(1->3)-beta-D-Galp-(1->]n, Controlled, tTransglutaminase P, LC-MS2, 293 HEK, Medobiotin, Controlling, preventive therapy, Rombellin, protein complex, 4B, Modern, DT, Proteins, LC-MS/MS, TGase-4 protein, Cell Lines, Roche, Bullous pemphigoid antigen 1, Biotin Gelfert, Bullous pemphigoid antigen, Dianin's compound, Procedure, 5-(2-oxohexahydro-1H-thieno[3, CATX-15, Cell, 4]imidazole-4-valeric acid, Peptide, 4S, 4-d)imidazole-4-pentanoic acid, (3aS-(3aalpha, 2-Bis(4'-hydroxyphenyl)propane, PSF, HSAN6, LC/MS/MS, cis-Tetrahydro-2-oxothieno(3, 4]imidazoline-4-valeric acid, biotin, native protein, natural protein, [4)-3, Protein, Sepharose C1, Biotin Ratiopharm, BPA, Bpa, 4-d)imidazoline-4-valeric acid, 2-Bis(p-hydroxyphenyl)propane, D-Biotin, Biodermatin, Lines, 2-Di(4-phenylol)propane, Biotin-Ratiopharm, 1H-Thieno(3, 4'-Bisphenol A, Biotine Roche, Gabunat, 4'-(Propane-2, 293, prostate transglutaminase, prophylaxis, MCGF, hexahydro-2-oxo-, HEK293, Methodological, HEK-293, 4)imidazole-4-valeric acid, Methodological Study, human, DMH, transglutaminase-4, plan specification, Protein Gene Products, Gene Proteins, BP240, control, liquid chromatography-tandem mass spectroscopy, Modern Man, EBSB2, XAP104, Sepharose 4B, cis-(+)-Tetrahydro-2-oxothieno[3, TGM4 protein, liquid chromatography tandem mass spectrometry, Peptid, Polypeptide, 293., 230|240 kDa bullous pemphigoid antigen, D-(+)-biotin, Biokur"],"name_synonyms":["tTransglutaminase P, 293 cell, 293 HEK, Medobiotin, 4-d]imidazol-4-yl)pentanoic acid, human being, Deacura, vitamin B7, Rombellin, Biotine, Modern, TGase-4 protein, Medebiotin, transglutaminase iv, Roche, Biotin Gelfert, Vitamin H, 5-(2-oxohexahydro-1H-thieno[3, 4]imidazole-4-valeric acid, 4S, 4-d)imidazole-4-pentanoic acid, Human, (3aS-(3aalpha, Biodermatin., cis-Tetrahydro-2-oxothieno(3, transglutaminase TGM4, 4]imidazoline-4-valeric acid, biotin, Homo sapiens, Biotin, 4beta, Biotin Ratiopharm, 6aR)-Hexahydro-2-oxo-1H-thieno[3, HEK 293, Human Embryonic Kidney 293, RCB1637, 4-d)imidazoline-4-valeric acid, 6aalpha))-, Gelfert, Man, D-Biotin, Biotin-Ratiopharm, 1H-Thieno(3, Biotin Hermes, Biotine Roche, Man (Taxonomy), Gabunat, Coenzyme R, 293, prostate transglutaminase, (3aS, cis-Hexahydro-2-oxo-1H-thieno(3, HEK293, biotina, hexahydro-2-oxo-, biotine, HEK-293, 4)imidazole-4-valeric acid, man, human, transglutaminase-4, 4-d]imidazole-4-valeric acid, Hermes, (+)-cis-Hexahydro-2-oxo-1H-thieno[3, Modern Man, cis-(+)-Tetrahydro-2-oxothieno[3, TGM4 protein, biotinum, D-(+)-biotin, Biokur"],"citation_count":["0"],"additional_accession":["PXD028174"]},"is_claimable":false,"name":"Identification of human transglutaminase 4 substrates in AD-293 protein extract by biotin-pentylamine incorporation","description":"We have identified cellular substrates of human transglutaminase 4 (TG4) by LC-MS/MS-based method from transglutaminase negative AD-293 cell line. Recombinant human TG4 dependent biotin pentylamine (BPA) modified peptides/proteins were detected from cytoplasmic (Cp) and nuclear (N) protein fractions of AD-293 cells (performed with 3 parallels). Control shows the neutravidin agarose interactome of AD-293 protein extract.","dates":{"publication":"Fri Aug 27 15:25:00 BST 2021"},"accession":"MSV000088030","cross_references":{}}