MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v04/MSV000088174/primaryOK2000000ProteomicsMichael MartySynapt XSBos Taurus (ncbitaxon:9913)Escherichia Coli (ncbitaxon:562)Homo Sapiens (ncbitaxon:9606)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=a7329b68a26c469e9e459d42ceac09famtmarty@arizona.eduUniversity of ArizonaMassIVE1,014UNIMOD:35 - "Oxidation or Hydroxylation."Antimicrobial peptides (AMPs) are an important part of the innate immune system and demonstrate promising applications in the fight against antibiotic-resistant infections due to their unique mechanism of targeting bacterial membranes. However, it is challenging to study the interactions of these peptides within lipid bilayers, making it difficult to understand their mechanisms of toxicity and selectivity. Here, we used fast photochemical oxidation of peptides, an irreversible footprinting technique that labels solvent accessible residues, and native charge detection-mass spectrometry to study AMP-lipid interactions with different lipid bilayer nanodiscs. We observed differences in the oxidation of two peptides, indolicidin and LL-37, in three distinct lipid environments, which reveal their affinity for lipid bilayers. Our findings suggest that indolicidin interacts with lipid head groups via a simple charge-driven mechanism, but LL-37 is more specific for <i>Escherichia coli</i> nanodiscs. These results provide complementary information on the potential modes of action and lipid selectivity of AMPs.Investigating Antimicrobial Peptide-Membrane Interactions Using Fast Photochemical Oxidation of Peptides in Nanodiscs.Reid Deseree J DJ, Rohrbough James G JG, Kostelic Marius M MM, Marty Michael T MTlipids, dairy cow, Bru, human being, Raw, determination, antibiotique, antimicrobials, antimicrobial agents, Lasers, composed of, HOA, extracted material, prevention, Human, Polypeptides, microbicides, peptido, Pc-G, Homo sapiens, cattle, Pulsed, Del(8)44H, Enterococcus coli, Masers, prevention and control, Man, antibiotics, 4-hydroxy-2-ketovalerate aldolase activity, Svc, antimicrobial, Pulsed., Maser, Man (Taxonomy), Escherichia/Shigella coli, Pulsed Lasers, peptides, reference sample, Antibiotika, Bos bovis, DmelCG32443, composition, dPC, DmPc, E. coli, CG32443, DmpG, Continuous Wave, 4-hydroxy-2-oxopentanoate pyruvate-lyase (acetaldehyde-forming) activity, preventive measures, Bos Tauurus, Indolicidin, Continuous Wave Laser, Q-Switched Lasers, oxen, peptidos, domestic cow, Controlled, Antibiotikum, antibiotic, Controlling, Bacterium coli, preventive therapy, cow, Modern, Continuous Wave Lasers, Q Switched Lasers, PcG, compositionality, Peptide, light amplification by the stimulated emission of radiation, Bos primigenius taurus, Bacillus coli, chemical analysis, Lipid, microbicide, domestic cattle, Q-Switched Laser, isoleucyl-leucyl-prolyl-tryptophyl-lysyl-tryptophyl-prolyl-tryptophyl-tryptophyl-prolyl-tryptophyl-arginyl-argininamide, Col4a-1, prophylaxis, content, Pulsed Laser, H-Ile-Leu-Pro-Trp-Lys-Trp-Pro-Trp-Trp-Pro-Trp-Arg-Arg-NH2, human, Q-Switched, Bacterium coli commune, ox, control, Modern Man, structure, Peptid, PC, 4-hydroxy-2-oxovalerate pyruvate-lyase activity, assay, Polypeptide, Laser, bovine, 4-hydroxy-2-oxopentanoate pyruvate-lyase activity, humans, CG7618Anhydrous ampicillin, 2'-AMP, DmelCG8604, ADENOSINE MONOPHOSPHATE, Adenosine 5' Phosphate, 2'-Adenosine Monophosphate, Adenosine 3'-Phosphate, dAmph, amph, (2S, APRTD, Adenosine Phosphate Dipotassium, Phosphate Disodium, Adenylic Acid, Adenosine 2' Phosphate, 5' Adenylic Acid, 5'-Adenosine monophosphate, PAdo, adenosine 5'-(dihydrogen phosphate), 2'-Adenosine, 2'-Adenylic Acid, Adenosine 3' Phosphate, Damp, ampicillin, 6-(D-(2-amino-2-phenylacetamido))-3, AMP, Amp, damph, 3-dimethyl-7-oxo-4-thia-1-azabicyclo(3.2.0)heptane-2-carboxylic acid, ampicillin acid, 2'-Adenylic, AP, 5'-Adenylic Acid, Adenosine-5'-monophosphoric acid, fosfato de adenosina, CG8604, 3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid, aminobenzylpenicillin, Adenosine Phosphate, D-(-)-ampicillin, 5'-Adenylic, D-(-)-6-(alpha-aminophenylacetamido)penicillanic acid, 6R)-6-{[(2R)-2-amino-2-phenylacetyl]amino}-3, 5R, Adenosine 5'-phosphate, Acid, 6R)-6-{[(2R)-2-amino-2-phenylethanoyl]amino}-3, 5'-AMP, ampicillin anhydrous, 3-DIMETHYL-7-OXO-4-THIA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC ACID, 2' Adenosine Monophosphate, Adenylate, Phosphate Dipotassium, ABPC, adenosine-5'P, pA, Adenosine 5'-Phosphate, Phosphaden, Papers., 6R)-6-{[(2R)-2-AMINO-2-PHENYLETHANOYL]AMINO}-3, Disodium, 5'-O-phosphonoadenosine, adenosine phosphate, Adenosine 2'-Phosphate, ampicillinum, 5'-Adenylic acid, phosphate d'adenosine, Adenosine, Adenosine Phosphate Disodium, Dipotassium, ampicilline, ampicilina, Ado5'P, spindle cells, 5'-Phosphate, 2' Adenylic Acid, Monophosphate, adenosini phosphas, Adenylic acid, DAMPAdenosine 5' Phosphate, Immune Systems, dAmph, APRTD, antimicrobials, Compounds, Adenylic Acid, Infestations and Infections, antimicrobial agents, bacterium E3, Antimicrobial Peptide, 5'-Adenosine monophosphate, PAdo, Solvent, Membrane Tissues, Polypeptides, 6-(D-(2-amino-2-phenylacetamido))-3, Techniques, Generic Action, microbicides, damph, peptido, Anti Mycobacterial Agent, Method, ampicillin acid, AP, Adenosine-5'-monophosphoric acid, symptoms, Impacts, aminobenzylpenicillin, Analysis, Enterococcus coli, Environmental Impacts, Bacteriocide, Bilayers, Mass Spectrum Analysis, antimicrobial, Antimycobacterial Agents, ampicillin anhydrous, 3-DIMETHYL-7-OXO-4-THIA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC ACID, Escherichia/Shigella coli, peptides, Analyses, Antibiotika, Tissue, Anti Bacterial Compound, E coli, Heads, ASL, adenosine phosphate, Fast, 5'-Adenylic acid, Anti-Bacterial Compounds, phosphate d'adenosine, E. coli, Eschericia coli, Enteroaggregative Escherichia coli, Environmental Impact, Indolicidin, Immune, Methodological Studies, ampicilina, head (volume), Antimycobacterial Agent, Infections and Infestations, SIMPLE, FAST, antibiotic, Anhydrous ampicillin, Membrane Tissue, screening, Adl, 2'-Adenosine Monophosphate, Adenosine 3'-Phosphate, (2S, Adenosine Phosphate Dipotassium, FASTK, Antibacterial Agent, Procedure, Enteroaggregative E. coli, Spectrum Analysis, 2'-Adenylic Acid, Adenosine 3' Phosphate, Spectroscopy, 3-dimethyl-7-oxo-4-thia-1-azabicyclo(3.2.0)heptane-2-carboxylic acid, Diffusely Adherent E. coli, PIG7, Lipid, 2'-Adenylic, margin of safety, fosfato de adenosina, 3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid, microbicide, Anti Bacterial Agent, D-(-)-6-(alpha-aminophenylacetamido)penicillanic acid, Bacteriocides, Anti-Mycobacterial Agent, Antimycobacterial, 5R, Acid, Action, Diffusely Adherent Escherichia coli, Enteroinvasive E. coli, Antibacterial, System, Spectrometry, Adenosine 5'-Phosphate, signs, Infection and Infestation, Phosphaden, 6R)-6-{[(2R)-2-AMINO-2-PHENYLETHANOYL]AMINO}-3, Methodological, 5'-O-phosphonoadenosine, Methodological Study, ampicillinum, Antibacterial Agents, Adenosine, Agents, Charge, Charges and Fees, spindle cells, Polypeptide, ASASE., Monophosphate, adenosini phosphas, DAMP, DmelCG8604, lipids, Procedures, amph, antibiotique, Phosphate Disodium, STK10, Adenosine 2' Phosphate, Spectrum Analyses, adenosine 5'-(dihydrogen phosphate), Damp, Agent, method, method used in an experiment, Escherchia coli, Studies, Mass, AMPS, Antibiotic, Bacteriocidal, Enteroinvasive Escherichia coli, 5'-Adenylic, Technique, 6R)-6-{[(2R)-2-amino-2-phenylacetyl]amino}-3, antibiotics, Mass Spectroscopy, study, 5'-AMP, 2' Adenosine Monophosphate, Adenylate, Tissues, cephalic area, Anti Bacterial Compounds, Charges, ASASE, Anti-Mycobacterial, Study, Infestation and Infection, Anti-Bacterial, ampicilline, Fee, Anti-Mycobacterial Agents, ATP:Fas-activated serine/threonine protein phosphotransferase activity, Ado5'P, peptidos, 5'-Phosphate, Antibiotics, toxic potential, Antibiotikum, TP53I7, Antimicrobial, Alkalescens-Dispar Group, Lipid Bilayer, 2'-AMP, ADENOSINE MONOPHOSPHATE, Bacterium coli, findings, 5' Adenylic Acid, EAggEC, 2'-Adenosine, Peptide, Anti Mycobacterial Agents, Impact, Environmental, ampicillin, AMP, Amp, MS, Bacillus coli, Systems, 5'-Adenylic Acid, Infection, CG8604, Adenosine Phosphate, Bacteriocidal Agents, D-(-)-ampicillin, Mass Spectrum Analyses, Adenosine 5'-phosphate, Mass Spectrum, 6R)-6-{[(2R)-2-amino-2-phenylethanoyl]amino}-3, isoleucyl-leucyl-prolyl-tryptophyl-lysyl-tryptophyl-prolyl-tryptophyl-tryptophyl-prolyl-tryptophyl-arginyl-argininamide, Anti Bacterial Agents, Phosphate Dipotassium, ABPC, adenosine-5'P, pA, Disodium, Membrane, H-Ile-Leu-Pro-Trp-Lys-Trp-Pro-Trp-Trp-Pro-Trp-Arg-Arg-NH2, Adenosine 2'-Phosphate, Bacteriocidal Agent, plan specification, Anti-Bacterial Agent, Adenosine Phosphate Disodium, Dipotassium, Bacterium coli commune, Compound, Fees, Bilayer, Environments, Peptid, 2' Adenylic Acid, Anti-Bacterial Compound, adult head, Adenylic acidAntimicrobial, Membrane Tissue, membrane, transmembrane, membrane of organ, peptides, Tissues, membrane region, Tissue, STK10, integral to membrane, Antimicrobial Peptide, FASTK, Membrane, membranous organ component, Peptide, Fast, Membrane Tissues, Polypeptides, peptido, ATP:Fas-activated serine/threonine protein phosphotransferase activity, integral component of membrane, region of membrane, whole membrane, Peptid, peptidos, Polypeptide, Peptide., antimicrobial peptide, FAST0falseFPOP AMP-Nanodisc Paper Reid et. Al. 2021Raw and processed data from FPOP analysis of bovine indolicidin and human LL-37 antimicrobial peptides with nanodiscs composed of DMPC, DMPG, or E. coli polar lipid extract. Data is provided for both oxidized samples and control samples with no exposure to the UV laser. MSV00008817434866389