{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v04/MSV000088579/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Jose Thalles Lacerda","Leonardo de Assis"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=dff1a83a2518479e9884ac20625f3701"],"submitter_email":["thalles_lacerda2@hotmail.com","leonardo.deassis@uni-lubeck.de"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["10"],"ptm_modification":["UNIMOD:1 - \"Acetylation.\"","UNIMOD:35 - \"Oxidation or Hydroxylation.\"","UNIMOD:6 - \"Iodoacetic acid derivative.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["maXis"],"species":["Mus Musculus (ncbitaxon:10090)"],"submitter_affiliation":["University of Sao Paulo","University of Luebeck"],"pubmed_abstract":["The search for new therapeutical targets for cutaneous melanoma and other cancers is an ongoing task. We expanded this knowledge by evaluating whether opsins, light- and thermo-sensing proteins, could display tumor-modulatory effects on melanoma cancer. Using different experimental approaches, we show that melanoma cell proliferation is slower in the absence of Opn4, compared to Opn4<sup>WT</sup> due to an impaired cell cycle progression and reduced melanocyte inducing transcription factor (Mitf) expression. In vivo tumor progression of Opn4<sup>KO</sup> cells is remarkably reduced due to slower proliferation, and higher immune system response in Opn4<sup>KO</sup> tumors. Using pharmacological assays, we demonstrate that guanylyl cyclase activity is impaired in Opn4<sup>KO</sup> cells. Evaluation of Tumor Cancer Genome Atlas (TCGA) database confirms our experimental data as reduced MITF and OPN4 expression in human melanoma correlates with slower cell cycle progression and presence of immune cells in the tumor microenvironment (TME). Proteomic analyses of tumor bulk show that the reduced growth of Opn4<sup>KO</sup> tumors is associated with reduced Mitf signaling, higher translation of G2/M proteins, and impaired guanylyl cyclase activity. Conversely, in Opn4<sup>WT</sup> tumors increased small GTPase and an immune-suppressive TME are found. Such evidence points to OPN4 as an oncogene in melanoma, which could be pharmacologically targeted."],"pubmed_title":["Melanopsin (Opn4) is an oncogene in cutaneous melanoma."],"pubmed_authors":["de Assis Leonardo Vinícius Monteiro LVM, Lacerda José Thalles JT, Moraes Maria Nathália MN, Domínguez-Amorocho Omar Alberto OA, Kinker Gabriela Sarti GS, Mendes Davi D, Silva Matheus Molina MM, Menck Carlos Frederico Martins CFM, Câmara Niels Olsen Saraiva NOS, Castrucci Ana Maria de Lauro AML"],"pubmed_title_synonyms":["cutaneous melanoma, Melanoma of the Skin, Malignant Melanoma of Skin, melanoma of the skin, Malignant melanoma of skin (disorder), mouse, Melanoma of skin, malignant scalp melanoma, malignant melanoma of skin stage unspecified, MM - Malignant melanoma of skin, Malignant melanoma of other specified skin site, zone of skin melanoma (disease), unspecified (disorder), malignant lower limb melanoma, opsin 4 (melanopsin) protein, cancer of skin, Melanoma of Skin, site unspecified, rat, Opn4 protein, unspecified, Melanoma, malignant melanoma of skin, melanoma, stage unspecified, malignant neck melanoma, NOS, malignant melanoma of skin of trunk except scrotum, Malignant melanoma of skin NOS (disorder), Stage Unspecified, Skin, malignant melanoma (of skin), Malignant Melanoma (of Skin), melanoma of skin, Malignant melanoma of skin, Malignant melanoma of skin of other and unspecified parts of face, malignant lip melanoma, malignant ear melanoma, Malignant Cutaneous Melanoma, skin, malignant trunk melanoma, malignant melanoma of ear and/or external auricular canal, opsin 4, Malignant melanoma of other specified skin site (disorder), zone of skin melanoma, MALIG MELANOMA SKIN NEC, human, cutaneous melanoma (disease), malignant melanoma of skin of lower limb, OPN4 protein, melanoma (disease) of zone of skin, [X]Malignant melanoma of skin, Malignant melanoma of other specified sites of skin, MALIG MELANOMA SKIN NOS, cutaneous (skin) melanoma, malignant cutaneous melanoma, malignant melanoma of skin of upper limb, malignant upper limb melanoma, MAL MELANOM FACE NEC/NOS., melanopsin, skin melanoma"],"pubmed_abstract_synonyms":["Melanoma of the Skin, protein translation, WS2A, T-cell leukemia, guanyl cyclase activity, Immune Systems, FON1, postnatal development, AGL4, malignant scalp melanoma, Monomeric G-Protein, Visible Light, growth and development, Tumor, FLORAL ORGAN NUMBER 1, brl, vit, cancer of skin, Small, ras Related GTP Binding Proteins, unspecified, GTPases, Small GTPases, melanoma, GTP diphosphate-lyase (cyclizing, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, responsivity, hnu, SUP, malignant melanoma of skin of trunk except scrotum, GTP-Binding Protein, Tumor Microenvironments, malignant melanoma cell, foton, ras Related G Proteins, small G-protein, Cell Division Cycles, increased, Man (Taxonomy), Genomes, C1, BCC2, Bdr, hypoplasia, Vitiligo, F10N7_150, 3', ras-Related, Malignant melanoma of other specified skin site (disorder), bw, MALIG MELANOMA SKIN NEC, Super protein, Dietary Fiber, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, sp, SNAP-25, Small G Proteins, K2p3.1, [X]Malignant melanoma of skin, TNFSF14, Immune, malignant neoplasm, malignant melanoma of skin of upper limb, Cell Cycles, AGAMOUS-like 4, Malignancies, zinc ion regulated proximal promoter sequence-specific DNA binding, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, TRANSCRIPTION FACTOR, gamma, single organism signaling, Tumors, RNA polymerase II distal enhancer sequence-specific binding, Cancer Microenvironments, cutaneous melanoma, close to, RNA polymerase II core promoter proximal region sequence-specific binding, protein anabolism, GTP Binding Proteins, Radiation, protein biosynthetic process, melanoma of the skin, UNQ391/PRO726, Modern, Light, small GTPase activity, Malignant Melanomas, small monomeric GTPase activity, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, MM - Malignant melanoma of skin, 5'-cyclic-GMP-forming) activity, Malignant melanoma of other specified skin site, Division Cycles, Expanded, ras-Related GTPases, malignant lower limb melanoma, Melanoma, TBAK1, LIGHT, Benign, malignant melanoma of skin, stage unspecified, malignant neck melanoma, 1270, protein formation, GENA70, ras-Related G-Proteins, NOS, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, Visible Radiations, F14P3.4, Malignant melanoma of skin of other and unspecified parts of face, malignant lip melanoma, Epistemology, Visible Radiation, absence, Factors, malignant ear melanoma, HVEML, PPH4, Division Cycle, growth pattern, non-developmental growth, System, expanded, F14P3_4, Ex, Benign Neoplasms, WS2, whole genome, Small G-Proteins, Transcription factor, human, Malignant Neoplasms, experimental procedures, Atlases, G Proteins, MALIG MELANOMA SKIN NOS, light quantum, enlarged, protein synthesis, cutaneous (skin) melanoma, guanylyl cyclase activity, receptor guanylate cyclase activity, Monomeric, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, l(2)ey, Wh, TASK-1, other neoplasm, metal ion regulated sequence-specific DNA binding, metal ion regulated proximal promoter sequence-specific DNA binding, humans, accessory, MAL MELANOM FACE NEC/NOS, FLORAL DEFECTIVE 10, GTP diphosphate-lyase (cyclizing) activity, big, heterotrimeric G-protein GTPase activity, human being, Malignant melanoma of skin (disorder), experimental, Microenvironment, Neoplasms, Benign Neoplasm, number, small monomeric G protein activity, Gene, SUPERMAN, large G-protein GTPase activity, Bhlhe32, Monomeric G Protein, SEPALLATA 2, ras-Related GTP-Binding Proteins, Malignant, Transcription Factor, presence, supernumerary, zone of skin melanoma (disease), unspecified (disorder), Ly113, Human, melanophore, site unspecified, melanoma (disease), large, DmelCG4114, zinc ion regulated core promoter proximal region sequence-specific DNA binding, reduced, Homo sapiens, Cancer Microenvironment, Ras superfamily protein, Cycle, Gene Products, tiny, Malignant melanoma of skin NOS (disorder), Stage Unspecified, Man, GTP-Binding Proteins, Malignant Melanoma (of Skin), melanoma of skin, Malignant melanoma of skin, Cycles, reactivity, Transcription, methods, Melanocyte, skin, Malignancy, malignant trunk melanoma, Lichtquant, experimental section, ras Related GTPase, Naevocarcinoma, absent from organism, Cell Division Cycle, Small G Protein, cell-division cycle, ras-Related GTP-Binding, zone of skin melanoma, Cell Division, Visible, small GTPase, melanoma (disease) of zone of skin, Neoplasias, GTP diphosphate-lyase (cyclizing -  3', Mif1, Malignant melanoma of other specified sites of skin, RNA polymerase II proximal promoter sequence-specific DNA binding, CMM8, great, copper ion regulated proximal promoter sequence-specific DNA binding, photon, G-Proteins, Monomeric G Proteins, bHLHe32, skin melanoma, TR2, Monomeric GTP Binding Proteins, ras Related GTP Binding Protein, GTPase, Cancer, small, G-Protein, ras Related GTPases, Malignant Melanoma of Skin, Malignant Neoplasm, large G-protein activity, OAT1, malignant, Proteins, Melanoma of skin, CG4114, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, Synaptosomal-associated 25 kDa protein, Factor, malignant melanoma of skin stage unspecified, Naevocarcinoma., CD258, MI, mi, Cell, near to, transcription factor activity, Melanoma of Skin, TASK, development, Melanomas, count in organism, ras-Related GTP-Binding Protein, MT, Malignant Melanoma, Protein, Systems, Neoplasm, RNA polymerase II transcription factor activity, protein biosynthesis, Small GTPase, Skin, Opsin, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, KNOTTED1-like homeobox gene 5, malignant melanoma (of skin), Data Base, ras-Related GTPase, Radiations, Monomeric G-Proteins, primary cancer, Malignant Cutaneous Melanoma, underdeveloped, HVEM-L, increased number, malignant melanoma of ear and/or external auricular canal, Photoradiation, postnatal growth, Small G-Protein, FLO10, Cancers, metal ion regulated core promoter proximal region sequence-specific binding, HERP, copper ion regulated core promoter proximal region sequence-specific binding, malignant tumor, cutaneous melanoma (disease), F10N7.150, malignant melanoma of skin of lower limb, LTg, l(2)01270, Protein Gene Products, Gene Proteins, present in greater numbers in organism, Photoradiations, signalling process, Gsfbcc2, malignant cutaneous melanoma, approaches, SNAP, malignant upper limb melanoma, Modern Man, vicinity of, malignant melanoma, response, Microenvironments, growth, Neoplasia"],"name_synonyms":["cutaneous melanoma, Melanoma of the Skin, Malignant Melanoma of Skin, melanoma of the skin, Malignant melanoma of skin (disorder), experimental, mouse, Melanoma of skin, malignant scalp melanoma, malignant melanoma of skin stage unspecified, MM - Malignant melanoma of skin, Malignant melanoma of other specified skin site, zone of skin melanoma (disease), unspecified (disorder), malignant lower limb melanoma, opsin 4 (melanopsin) protein, cancer of skin, Melanoma of Skin, site unspecified, rat, Opn4 protein, unspecified, Melanoma, malignant melanoma of skin, melanoma, stage unspecified, malignant neck melanoma, NOS, malignant melanoma of skin of trunk except scrotum, Malignant melanoma of skin NOS (disorder), Stage Unspecified, Skin, malignant melanoma (of skin), Malignant Melanoma (of Skin), melanoma of skin, Malignant melanoma of skin, Malignant melanoma of skin of other and unspecified parts of face, malignant lip melanoma, methods, malignant ear melanoma, Malignant Cutaneous Melanoma, skin, malignant trunk melanoma, experimental section, malignant melanoma of ear and/or external auricular canal, opsin 4, study., Malignant melanoma of other specified skin site (disorder), zone of skin melanoma, MALIG MELANOMA SKIN NEC, human, cutaneous melanoma (disease), malignant melanoma of skin of lower limb, experimental procedures, OPN4 protein, melanoma (disease) of zone of skin, [X]Malignant melanoma of skin, Malignant melanoma of other specified sites of skin, MALIG MELANOMA SKIN NOS, cutaneous (skin) melanoma, malignant cutaneous melanoma, malignant melanoma of skin of upper limb, malignant upper limb melanoma, melanopsin, skin melanoma, MAL MELANOM FACE NEC/NOS"],"description_synonyms":["close to, study, Peptidomics, malignant, Naevocarcinoma, postnatal development, postnatal growth, Goal, growth and development, Malignant Melanomas, Malignant, Concept, near to, development, melanoma (disease), Melanomas, Role Concept, Melanoma, melanoma, Roles, approaches, Malignant Melanoma, Role Concepts, Role, Concepts, vicinity of., malignant melanoma, growth"],"citation_count":["0"],"additional_accession":["PXD030477"]},"is_claimable":false,"name":"Melanopsin (OPN4) is a novel oncogene in cutaneous melanoma: evidence from an experimental study ","description":"The goal of this study was to evaluate the role of OPN4 in melanoma development using in vitro, in vivo, and proteomics approaches.","dates":{"publication":"Fri Dec 17 04:13:00 GMT 2021"},"accession":"MSV000088579","cross_references":{"pubmed":["35562405"]}}