MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v04/MSV000088590/primary200OK0000ProteomicsBenjamin C OrsburnExactiveQ ExactiveHomo Sapiens (ncbitaxon:9606)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=41b9348868544bfd831127e0d35e0e6cborsbur1@jhmi.eduJohns HopkinsMassIVE7MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset10kDa, Hsp84-1, AU043053, Procedures, protein complex, 1110005L13Rik, Proteins, FBN, Gene, protein, AL022974, Procedure, protein-containing complex, Monoclonal, C81438, ACMICD, protein polypeptide chains, method, Techniques, Antibody, Experiment, AI848992, native protein, Hsp10, natural protein, polypeptide chain, Monoclonal Antibodies, ECTOL1, Method, method used in an experiment, Protein, Gene Products, Studies, MFS1, protein aggregate, Sf3b10, p90rsk, Technique, WMS, WMS2, 1110069D02Rik, pp90rsk, Msk2, Hsp84, Hsp90, proteins, MASS, Methodological, Methodological Study, SCAN, D17Wsu134e, Protein Gene Products, OCTD, plan specification, Study, Gene Proteins, Methodological Studies, Hspcb, Rps6ka-rs1, Monoclonal Antibody., 90kDa, Isolation of Nuclei TAgged in specific Cell Types, SSKS, Rsk3, variable, GPHYSD2, SGS, mt-cpn10, mMSK2Gene., cycline, Effects, protein complex, DmcyclinE, fond, Proteins, Longterm Effect, cycE, protein, CycEI, Cyc E, br37, protein-containing complex, l(2)br37, Long Term, CYCLE, cdi7, protein polypeptide chains, l(2)k02514, Ccne, DmCycE, cyclinE, BG:DS07108.3, native protein, natural protein, polypeptide chain, Long Term Effects, Protein, Cdi7, CDI7, Gene Products, CyeE, protein aggregate, l(2)05206, Effect, centric, CYCE, l(2)k02602, Longterm, DmelCG3938, CyclE, l(2)35Dd, 3938, proteins, Long-Term, DmcycE, SCAN, Longterm Effects, Protein Gene Products, l(2)k05007, D-CycE, Gene Proteins, dm-cycE, l35Dd, Isolation of Nuclei TAgged in specific Cell Types, Long-Term Effect, CG3938, Long-Term Effects0falseMulti-scan cycle time centric optimization allows rapid intact protein and top down characterizationThe ionization conditions necessary for intact protein characterization in Orbitrap mass analyzers can be highly variable. To more rapidly characterize intact proteins we have developed a method where multiple sets of conditions can be used within a single experiment. These 3 scan events contain generic parameters for: 1) Proteins that do not require additional in-source collision energy for efficient ionization, 2) Proteins that require some additional energy, 3) Proteins that require substantial in source energy. We test this method with a commercial intact protein standard containing proteins between 10kDa and 90kDa with and without the addition of the NIST monoclonal antibody standard. Mon Dec 20 02:22:00 GMT 2021MSV000088590