MassIVEapplication/xmlftp://massive.ucsd.edu/MSV000088673/primaryOK2000001OtherJoana Paula da Silva OliveiraGeisa Paulino Caprini EvaristoAndrea Furtado MacedoQ Exactive PlusCyrtopodium Glutiniferum (ncbitaxon:2019951)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=cd6521178d3b41e7955e65e545fc692bFiocruz Rond�nia/CEBIOUNIRIO - Federal University of the State of Rio de Janeirojoanapaulasoliveira@gmail.comgeisapc@gmail.comandrea.macedo@unirio.brMassIVE73M+H, M-H<h4>Ethnopharmacological relevance</h4>Extracts of orchids have been traditionally used as human phytotherapeutics. Cyrtopodium flavum, for example, due to the analgesic and anti-inflammatory properties, beside the capacity of heal skin lesions has been focus of research. Also Cyrtopodium glutiniferum, an orchid found in the Brazilian southeastern rainforest, is known to synthesize anti-inflammatory glucomannans in the pseudobulbs, as other potentially therapeutic compounds.<h4>Aim of the study</h4>We have reported the first metabolomic analysis focused on the phenols expression of the neotropical orchid Cyrtopodium glutiniferum Raddi, besides free radical scavenging, anti-inflammatory and antiproliferative activities, and the genotoxicity properties of the aqueous extract.<h4>Material and methods</h4>The metabolomics of C. glutiniferum aqueous extract was performed through UHPLC-MSⁿ acquisition. We have detected the scavenging potential of the extract using DPPH assay. The genotoxic potential was performed by Ames Test (0-5000 μg mL^-1) and micronucleous assay (0-5000 μg mL^-1) in RAW264.7 cells. The cytotoxic potential of the extract against RAW264.7 was tested by WST-1 assay (0-500 μg mL^-1). And after all, the RAW264.7 cells were treated with non-cytotoxic concentrations of C. glutiniferum (0-50 μg mL^-1) to evaluate the antiproliferative and anti-inflammatory potential, besides the mitochondrial activity.<h4>Results</h4>From the 55 molecules identified, 45.5% belonged to the phenolic compounds database from Phenol Explorer, 29% to an in-house Orchidaceae molecules database, and 25.5% to both. Among the identified phenolic compounds, 18 subclasses were discriminated, being phenanthrenes the most abundant. Doses-dependent of C. glutiniferum extracts were able to induce DPPH free radicals scavenging and also to increase TA100 His⁺ revertants, in metabolic environment, showing mutagenicity just in the highest concentration, of 5 mg/plate. On Eukaryotic cell models, the extract also has induced dose-response and time-response cytotoxicity against RAW264.7 macrophages, mainly after 48 h and 72 h, even though the extract has not been able to induce the increase of micronucleated cells and mitotic index alteration on Micronucleus assay. The activation and proliferation of macrophages cultures were downregulated after 24 h and 48 h by the non-cytotoxic concentrations of the extract in a dose-dependent manner.<h4>Conclusions</h4>The Cyrtopodium glutiniferum metabolomics, anti-inflammatory and anti-proliferative properties observed in this study suggest a therapeutic efficacy of the orchid extract applied in folk medicine.A clear emulsion and gel-type composition, which is an antiperspirant or deodorant composition which is a water-in-oil emulsion which comprises: a clear emulsion and gel-type cosmetic composition which is an antiperspirant and/or deodorant composition, which is a water-in-oil emulsion comprising: a) about 65% to about 92% of an aqueous solution of an effective amount of antiperspirant active; b) about 8% to about 35% of an oil phase consisting of silicones, emollients and a silicone emulsifying agent; and c) a water soluble, non-glycol component that raises the refractive index of said aqueous solution. wherein said composition is essentially free of simple glycols and low and middle chain alcohols; is described.Metabolomic analysis of Cyrtopodium glutiniferum extract by UHPLC-MS/MS and in vitro antiproliferative and genotoxicity assessment.Preparation of antiperspirant gels which obviates the use of simple glycolsAraújo-Lima Carlos Fernando CF, Paula da Silva Oliveira Joana J, Coscarella Isabella Leite IL, Aiub Claudia Alessandra Fortes CAF, Felzenszwalb Israel I, Caprini Evaristo Geisa Paulino GP, Macedo Andrea Furtado AFMA ZHUNING, BREWSTER DAVID ALLEN, SCAFIDI ANTHONY ALOYSIUSMetabonomic, assay, Metabonomics, MS2., Metabolomic, determination, extracted material, MS/MS, tandem MS, chemical analysisHuman, Rainforests, Forests, Tropical Rainforests, Temperate, Rain Forests, human being, Man (Taxonomy), Tropical Rainforest, Homo sapiens, Tropical, Rainforest, Modern Man, synthesize., Modern, Rain, Temperate Rainforest, Forest, Man, human, humans, Rain Forest, Temperate RainforestsArea, Forestlands, Forestland, Forested Areas, Woodlands, Forest, Woodland., Forested, Areas, Forested Areathe integument, Antinociceptive Agents, pelt, Activity, HSN1E, determination, Laboratory, Skn-li, FON1, Metabonomic, Metabonomics, wasted, FLORAL ORGAN NUMBER 1, extracted material, Orchid Family, Long Term, Speciality, Techniques, Method, Specialities, responsivity, SUP, Monocyte-Derived Macrophage, Phenol, Impacts, Research Activity, Environmental Impacts, Laboratory Research, Effect, integumentum commune, S1, Priorities, Rainforests, Carbolic Acid, Insurance Medicine, acide carbolique, Man (Taxonomy), Bdr, dermoid system, Oncidium, entire skin, Karbolsaeure, procedures, Anodynes, Antinociceptive, Super protein, Phalaenopsis, free, Rain Forest, sp, SNAP-25, Environmental Impact, Phenylic acid, Temperate, Methodological Studies, Tropical, medicine, scientific observation, Carbol, Fam91a1, Otf11, Medicine, Medicines, Research Priority, AV220772, CXXC finger protein 9, skin and subcutaneous tissue, Long-Term Effects, Phenylic alcohol, Phenic acid, Specialties, Indices, Index, Modern, Longterm Effect, Research Priorities, integumental organ, Procedure, Medical Specialties., results, acide phenique, Medical Speciality, Tropical Rainforests, ATP synthase D chain, BC033609, Specialty, GENA70, activation, Monocyte-Derived Macrophages, Research and Development, Phenolate, ADCADN, Mitochondrial, UNQ203/PRO229, Rainforest, DNA (cytosine-5-)-methyltransferase 1, Aryl alcohol, carbolic acid, Methodological, arenols, Methodological Study, human, mKIAA0493, Activities, Insurance Medicines, vertebrate epidermis, Sodium Salt, Agents, Cells, Cypripedium, Mitotic, entire integument, dermal system, a phenol, humans, Eukaryotic, FLORAL DEFECTIVE 10, Analgesic Drugs, dermis plus epidermis plus hypodermis, Forests, region of skin, human being, Procedures, Effects, Monocyte Derived Macrophages, Otf-11, SUPERMAN, orchid family, wst, mitochondrial, Temperate Rainforests, epidermis, Human, Aim, AIM, Medical Specialities, Free Radical, Homo sapiens, Sodium Phenolate, Studies, Temperate Rainforest, Metabolomic, Medical, Man, Technique, Skn-1a, Drugs, DNMT1, study, reactivity, FOCUS, skin, Tropical Rainforest, DNMT1_HUMAN, Research, Benzenol, Longterm, Bone Marrow-Derived Macrophage, Long-Term, SKIN, Study, Bone Marrow-Derived, API6, skin organ, Mif1, Rain Forests, drugs, Analgesic Agents, Oxybenzene, Analgesic, Long-Term Effect, Development and Research, Insurance, Family, DNA MTase HsaI, DNA (cytosine-5)-methyltransferase 1, Orchid, measuring, Oct-11a, Medical Specialty, synthesize, Eef1a, DNMT, Rain, skin zone, Hydroxybenzene, Synaptosomal-associated 25 kDa protein, Monocyte Derived, MCMT, Mitotic Indices, Cell, Impact, Environmental, skin plus hypodermis, Monocyte-Derived, Priority, Oct11, skin region, DNA methyltransferase HsaI, chemical analysis, Long Term Effects, Research Activities, Sodium, Skin-1a, techniques, tegument, Skin, Data Base, Macrophage, skin benign neoplasm, CXXC9, CT-2, Macrophages, vertebrate integument, PhOH, Bone Marrow Derived Macrophages, FLO10, HERP, CXXC-type zinc finger protein 9, Longterm Effects, Epoc-1, Eukaryotic Cell, portion of skin, Carbolsaeure, integument, concentration, Families, SNAP, Phenolate Sodium, Modern Man, Bone Marrow-Derived Macrophages, Environments, assay, response, Forest, General activity, Orchid Families, CLEC2C, m.HsaI, methodology0.00.01.00.00.001patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0lineage-covid19~0gpmdb~0metagenomics~0pfam~0varsite~0falseCyrtopodium glutiniferum Raddi_Atlantic ForestExtracts of orchids have been traditionally used as human phytotherapeutics. Cyrtopodium glutiniferum, an orchid found in the Brazilian southeastern rainforest, is known to synthesize anti-inflammatory and other potentially therapeutic compounds.Tue Jan 11 11:38:00 GMT 2022MSV00008867331982517