{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v04/MSV000089582/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Kevin Coombs"],"instrument_platform":["LTQ Orbitrap"],"species":["Homo Sapiens (ncbitaxon:9606)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=6ab5adab22534f6496aafb2fa7e91fdf"],"submitter_email":["Kevin.Coombs@umanitoba.ca"],"submitter_affiliation":["university of manitoba"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["8"],"ptm_modification":["[+229.163","C+57.021","K+229.163"],"data_protocol":[""],"description_synonyms":["projections, Viridae, protein metabolism, Memory, chronic obstructive pulmonary disease (COPD), Long Term, Social Controls, Receptor Mediated Signal Transduction, solute:solute exchange, DmelCG6376, T22F8_160, Coronaviruses, parenchyma of lung, ATGPR7, Chronic irreversible airway obstruction, symptoms, GRP8, GRP7, Analysis, Autonomous Replication, Cell Signaling, WMS, Animalia, Cell Division Cycles, multicellular organismal protein metabolic process, Analyses, Flu, dE2F-2, CAFL - Chronic airflow limitation, tissue necrosis, Pathways, beta-CoVs, ecotype, Social, GPIa*, disease (COPD), Vira, flu, Human Flu, Delayed Memory, MRC5 cell, Signal Transduction Pathways, autolysin activity, transcription from bacterial-type RNA polymerase promoter, SGS, single organism signaling, screening, chronic obstructive lung disease [Ambiguous], SARS-CoV-2 Viruses, Pathway, β-CoV, pulmo, animalia, lung parenchyma, Longterm Effect, SARS-CoV-2, Coronavirus 2, influenza with non-respiratory manifestation, Spectrum Analysis, signal transduction by trans-phosphorylation, Memories, ACMICD, CHRONIC OBSTRUCTIVE AIRWAY DIS, Repressed, SARS-CoV-2 Virus, Dp, shelf, l(3)07172, def21, Humoral, Airflow Obstruction, GLYCINE-RICH PROTEIN 8, Orthomyxoviruses, influenza, Chronic Obstructive Airways Disease, Chronic obstructive lung disease, whole organism, death rate, Spectrometry, signs, Controls, projection, ridge, human, Replications, F2G1.4, signaling pathway, pulmonary, OBSTRUCTIVE PULMONARY DISEASE (COPD), Koerper, DRTF1/E2F, bacteriolytic toxin activity, 2019, Animal Viruses, DNA, MRCV, humans, Regulations, MRC-5, Influenza with other manifestations, multi-cellular organism, Effects, False, lamellae, COVID-19, CAO - Chronic airflow obstruction, Myxovirus, CHRONIC OBSTRUCTIVE PULM DIS, Chronic obstructive pulmonary disease NOS, SARS CoV 2 Virus, process of organ, and RNA binding 1, COVID19 Virus, Human, Chronic obstructive pulmonary disease finding, lamella, Signal Transduction, PULMONARY DISEASE (COPD), SARS, False Memory Syndrome, lung, Gene Products, β-coronavirus, neoplasm, DNA Replications, Man, 2019-nCoV, l(3)rM729, MRC-V cell, Cycles, Animal Virus, SARS Coronavirus 2, β-CoVs, lipid metabolism, Rabbit, Delayed Memories, Longterm, Cell Division Cycle, cell-division cycle, Signal, inflammation, severe acute respiratory syndrome coronavirus 2, Signal Pathways, Long-Term, E2F, Signal Transduction Pathway, DmelCG1071, COVID-19 Virus, Medical Research Council cell strain-5, ATGRP7, ATGRP8, NEC, accidental cell death, Syndrome, Chronic, Influenza, laminae, Signal Pathway, protein metabolism and modification, findings, 2019 Novel Coronavirus, chronic obstructive airways disease NOS, beta-CoV, Rabbit Coronaviruses, body, Humoral Immune Response, COAD, Proteins, Receptor-Mediated Signal Transductions, whole body, e2f1, e2f2, e2f, COLD - Chronic obstructive lung disease, dE2f2, chronic, Cell, dE2f1, EMILIN4, MS, Severe Acute Respiratory Syndrome Coronavirus 2, Cytokine, Temperatures, Social Control, orthomyxoviridae caused disease or disorder, Long Term Effects, Strain, drosE2F1, de2f2, MFS1, de2f1, (COPD), chronic airway obstruction, signalling cascade, chronic obstructive, Death, CAL - Chronic airflow limitation, Mass Spectrum Analyses, organ process, covalent modifier, 2019 Novel, betacoronavirus, holin, CG1071, GR-RBP7, GR-RBP8, E2F1, E2F2, Memory Syndrome, Longterm Effects, Evar(3)164, processes, Gene Proteins, MRC-V, Psychology Repression, Humoral Immune, signalling process, chronic obstructive lung disease (disorder), regulation, E2f1, dE2F2, dE2F1, chronic obstructive pulmonary disease, Influenza in Human, host organism, Signal Transductions, T22F8.160, and rna binding 2, Chronic Airflow Obstructions, 2019 novel coronavirus, E(var)3-95E, Tier, Influenza Virus, chronic obstructive airways disease NOS (disorder), Novel Coronavirus, Rabbit Coronavirus, Virus, Chronic Obstructive Pulmonary Disease (COPD), animal, Effect, Formal Social Controls, Mass Spectrum Analysis, signal transduction by protein phosphorylation, Immune Responses, COLD (chronic obstructive lung disease), Man (Taxonomy), inflammatory response, NEC in ICD9CM_2006, Repression, E(var)3-93E, dE2F, Autonomous, Chronic airflow limitation, False Memory, GLYCINE RICH PROTEIN 7, papilla, Cell Cycles, Influenza in Humans, Strains, necrosis, Transductions, glycine-rich RNA-binding protein 8, GPHYSD2, Influenza with other manifestations (disorder), CHRONIC OBSTRUCTIVE PULMONARY DISEASE, Long-Term Effects, CHRONIC, Influenzas, obstructive lung disease, COVID-19 Viruses, Modern, lamina, signal transduction by conformational transition, flanges, CG6376, Airflow Obstructions, Division Cycles, Spectroscopy, pulmonary disease (COPD), organism, COVID19 Viruses, signaling cascade, Humoral Immunity, Influenza Viruses, single-organism transport, CHRONIC OBSTRUCTIVE, Repressed Memory, Lipid, chronic obstructive pulmonary disease and allied conditions, Chronic airway disease, bacteriocin activity, Coronavirus, NOS, cultivar, DmE2F-1, Transduction, dE2f, neoplasms, COPD NOS, CHRONIC OBSTRUCTIVE LUNG DIS, Chronic airway obstruction, E(var)93E, cold, Division Cycle, Human Influenza, Coronavirus Disease 2019 Virus, shelves, System, Control, influenza virus infection, MASS, l(3)j3C2, Chronic Airflow Obstruction, Delayed, lysin activity, COPD - Chronic obstructive pulmonary disease, bacterial transcription, chronic obstructive airways disease, small molecule transport, Autonomous Replications, obstructive pulmonary disease (COPD), l(3)j3B1, Strains and Sprains, Regulation, chronic obstructive lung disease, COLD, Orthomyxovirus, COVID19, Signal Transduction Systems, influenza infection, human being, Influenza with other manifestations NOS (disorder), DISEASE (COPD), MRC-5 cell, Receptor-Mediated, FBN, Gene, Signal Transduction System, circadian rhythm, Spectrum Analyses, Cold, Pulmonary Disease, Homo sapiens, ECTOL1, Metabolism, Cycle, Mass, Animal, Sprains, Mass Spectroscopy, 2019 Novel Coronaviruses, Cold Temperatures, Myxoviruses, Viruses, Wuhan Seafood Market Pneumonia Virus, metazoa, Cell Division, ridges, E2F-1, Chronic Obstructive, False Memory Syndromes, OCTD, signalling pathway, Immune Response, time of survival, ECM, signal transduction by cis-phosphorylation, Long-Term Effect, Influenza with non-respiratory manifestation (disorder), Grippe, TAG, Wuhan Coronavirus, orthomyxoviridae disease or disorder, COPD, protein metabolic process and modification, COAD - Chronic obstructive airways disease, Formal Social Control, not elsewhere classified, Sprain, PULM DIS CHRONIC OBSTRUCTIVE, Chronic obstructive pulmonary disease finding (finding), strain, viruses, survival, Receptor-Mediated Signal Transduction, Metazoa, Protein, Systems, E(Sev-CycE)3A, GLYCINE-RICH RNA-BINDING PROTEIN 7, Zoophaginae, Replication, flange, WMS2, chronic obstructive airway disease, Temperature, Mass Spectrum, Dops, Humoral Immune Responses, Wuhan, SARS-coronavirus 2, Protein Gene Products, orthomyxoviridae infectious disease, Human Influenzas, Rabbit., Modern Man, SSKS, Response, cold (chronic obstructive lung disease), Lungs, COVID 19 Virus, Chronic Obstructive Lung Disease, MMRN"],"name_synonyms":["Human, human being, Man (Taxonomy), pulmo, Rabbit, Homo sapiens, pulmonary, parenchyma of lung, Coronaviruses, Rabbit Coronaviruses, Rabbit., Modern Man, Modern, lung, Rabbit Coronavirus, lung parenchyma, Coronavirus, Lungs, neoplasm, Man, neoplasms, human, humans, Cell"],"additional_accession":[]},"is_claimable":false,"name":"Temporal Proteomic Analyses of Human Lung Cells Infected with Coronavirus OC43","description":"The coronaviruses consist of many animal and human pathogens, with some of the human coronavirus, such as strain OC43, normally causing only mild cold-like symptoms. Viruses usurp host cellular processes to successfully replicate. We used tandem mass tag mass spectrometry-based proteomic analyses of human lung MRC5 cells infected with OC43 for various periods of time to delineate virus-induced host cell alterations. Numerous proteins involved in lipid metabolism, molecular transport, small molecule biochemistry, cell death and survival, humoral immune response, and inflammatory response were dysregulated. Comparison of our data with previous studies that examined a continuum of differentially pathogenic influenza viruses, and with SARS-CoV-2 data from other studies, indicated that proteins involved in the cell cycle, cytokine signaling, DNA replication and anti-inflammatory responses were generally similarly affected by virtually all IAV and CoV tested, whereas proteins involved in necrosis, protein metabolism, ECM regulation and signal transduction were generally differentially affected by CoV and IAV. In addition, the more pathogenic CoV and IAV activated Rb-dependent repression of E2F-mediated transcription, whereas less pathogenic influenza and coronaviruses either inhibited or had no effect on this pathway.","dates":{"publication":"Wed Jun 01 13:02:00 BST 2022"},"accession":"MSV000089582","cross_references":{}}