{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v04/MSV000089866/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Laura M Sanchez","Joanna Burdette"],"instrument_platform":["autoflex II","timsTOF fleX","Q Exactive"],"species":["Homo Sapiens (ncbitaxon:9606)","Mus Musculus (ncbitaxon:10090)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=1e835a8628bc414fbcfe32361bc5b6a5"],"submitter_affiliation":["University of California, Santa Cruz","University of Illinois at Chicago"],"submitter_email":["joannab@uic.edu","lmsanche@ucsc.edu"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["1,069"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"pubmed_abstract":["High grade serous ovarian cancer (HGSOC), the most lethal histotype of ovarian cancer, frequently arises from fallopian tube epithelial cells (FTE). Once transformed, tumorigenic FTE often migrate specifically to the ovary, completing the crucial primary metastatic step and allowing the formation of the ovarian tumors after which HGSOC was originally named. As only the fimbriated distal ends of the fallopian tube that reside in close proximity to the ovary develop precursor lesions such as serous tubal intraepithelial carcinomas, this suggests that the process of transformation and primary metastasis to the ovary is impacted by the local microenvironment. We hypothesize that chemical cues, including small molecules and proteins, may help stimulate the migration of tumorigenic FTE to the ovary. However, the specific mediators of this process are still poorly understood, despite a recent growth in interest in the tumor microenvironment. Our previous work utilized imaging mass spectrometry (IMS) to identify the release of norepinephrine (NE) from the ovary in co-cultures of tumorigenic FTE cells with an ovarian explant. We predicted that tumorigenic FTE cells secreted a biomolecule, not produced or produced with low expression by non-tumorigenic cells, that stimulated the ovary to release NE. As such, we utilized an IMS mass-guided bioassay, using NE release as our biological marker, and bottom-up proteomics to demonstrate that a secreted protein, SPARC, is a factor produced by tumorigenic FTE responsible for enhancing release of ovarian NE and influencing primary metastasis of HGSOC. This discovery highlights the bidirectional interplay between different types of biomolecules in the fallopian tube and ovarian microenvironment and their combined roles in primary metastasis and disease progression."],"pubmed_title":["Fallopian tube secreted protein affects ovarian metabolites in high grade serous ovarian cancer."],"pubmed_authors":["Bergsten Tova M TM, Levy Sarah E SE, Zink Katherine E KE, Lusk Hannah J HJ, Pergande Melissa R MR, Cologna Stephanie M SM, Burdette Joanna E JE, Sanchez Laura M LM"],"pubmed_title_synonyms":["Tube, Mammalian Oviduct, Fallopian tube, protein complex, Uterine Tube, female paramesonephric duct, Mammalian, Salpinx, Proteins, secondary metabolites, Gene, tuba uterina, protein, Tubes, protein-containing complex, Uterine, protein polypeptide chains, primary metabolites, native protein, natural protein, polypeptide chain, Uterine Salpinx, Protein, uterine tube, Gene Products, salpinges, protein aggregate, tuba uterinae, grade 3/4, mammalian oviduct, Mammalian Oviducts, Fallopian Tube, metabolite, fallopian tube, uterine tube (sensu Mammalia), paramesonephric duct of female, proteins, Oviduct, high grade, Fallopian, Protein Gene Products, Salpinges, Gene Proteins, oviducts, Uterine Tubes, metabolites, Oviducts, malignant ovarian serous tumour, serous ovarian cancer., fallopian tubes, salpinx, Uterine Salpinges"],"pubmed_abstract_synonyms":["SPARC, Cocultures, Epithelial Cell, Metastasis, -)-Isomer, ovary tumor, BM-40 Sparc, Mammalian, postnatal development, Salpinx, Cue, Monohydrate, Neoplasm Metastases, growth and development, Imaging, protein, Osteonectin, neoplasm metastasis, neutral molecular compounds, BM40, Glandular, Tumor, Levonor, Noradrenaline, cancer metastasis, gonada of female organism genitalia, DmelCG6378, malignant tumour of ovary, protein polypeptide chains, ovarian cancer, Biological, GRP1, Grp1, 1, Analysis, Cocultivations, Tumor Microenvironments, protein aggregate, molecule, WMS, multicellular organismal biosynthetic process, genitalia of female organism gonada, molecula, Mass Spectrum Analysis, Progression, Norepinephrine Bitartrate, BM-40/SPARC, single-organism biosynthetic process, OVARY NEOPL, me75, molecules, Biologic Assays, Analyses, anabolism, ovum-producing ovary, PTPSTEP, Assays, hypoplasia, female organism reproductive system gonad, 2-Benzenediol, Adenomatous Epithelial, proteins, Molekuel, D17Mit170, high grade, primary ovarian cancer, T1, NEOPL OVARIAN, Salpinges, reproductive system of female organism gonada, oviducts, Disease., l(2)k08110, Oviducts, Exacerbation, Ovarian Cancers, GPHYSD2, CT19876, GPH, SGS, Transitional Epithelial Cell, Cancer Microenvironments, Tube, Cancer of Ovary, anatomical protrusion, gonada of reproductive system of female organism, gonad of female organism reproductive system, anon-EST:Posey291, female paramesonephric duct, Disease Exacerbation, Adenomatous, CG6378, Squamous, Tubes, Tl3, Transitional Epithelial, Tl2, Uterine, Spectrum Analysis, predicted, ACMICD, Spectroscopy, epithelial, Ovaries, malignant Ovarian tumor, 3.1.3.48, Norepinephrine l-Tartrate (1:2), Biologic Assay, female reproductive system gonad, Arterenol, Ovary Neoplasm, ovarian tumour, parent ion, grade 3/4, Adenomatous Epithelial Cell, Coculture, Adenomatous Epithelial Cells, Squamous Cell, gonada of genitalia of female organism, growth pattern, Step, CG11628, non-developmental growth, Norepinephrine l-Tartrate (1:1), gonad of reproductive system of female organism, Spectrometry, Levophed Bitartrate, Ovary Neoplasms, paramesonephric duct of female, Striatum-enriched protein-tyrosine phosphatase, MASS, ovary neoplasm, Fallopian, Biological Assays, metastatic, Noradrénaline tartrate renaudin, Bioassays, (+)-Isomer, female organism genitalia gonada, precursor ion, AA517111, STEP, spine, Cells, Levophed, Norepinephrine, BM-40, Ovary Cancer, (+, Mammalian Oviduct, GRP1/cytohesin 1, Microenvironment, Fallopian tube, Uterine Tube, Neoplasms, Levarterenol, FBN, Gene, tuba uterina, ovary tumour, tumor of ovary, neoplasm of ovary, Glandular Epithelial, biosynthesis, precursor, Spectrum Analyses, protein-containing complex, protrusion, CG11633, Levonorepinephrine, cytohesin/GRP1, OVARIAN NEOPL, ovarian tumors, gonad of genitalia of female organism, Squamous Epithelial Cells, reduced, polypeptide chain, Ovarian, Cancer Microenvironment, ECTOL1, Uterine Salpinx, Gene Products, Mass, Clinical Progression, Low, Basement-membrane protein 40, tiny, Squamous Epithelial, Co-culture, Mass Spectroscopy, Epithelial, tumor cell migration, gonada of female organism reproductive system, Clinical, Co-cultures, formation, genitalia of female organism gonad, Mammalian Oviducts, Ovarian Cancer, Fallopian Tube, l(2)SH2 0323, metastasis, tumour of ovary, Norepinephrine d-Tartrate (1:1), Oviduct, synthesis, OCTD, Neural-specific protein-tyrosine phosphatase, Clinical Course, reproductive system of female organism gonad, Epithelial Cells, Transitional Epithelial Cells, Norepinephrine l-Tartrate, ovary - animal, ovary, malignant ovarian serous tumour, biological marker, Norepinephrin d-Tartrate (1:1), fallopian tubes, serous ovarian cancer, salpinx, Squamous Epithelial Cell, female organism genitalia gonad, tumour of the ovary, tumor metastasis, Glandular Epithelial Cell, female reproductive system gonada, Biologic, Uterine Salpinges, Cancer, Transitional, small, Squamous Cells, Disease, 4-(2-amino-1-hydroxyethyl)-, gonad of female reproductive system, Coculture Technique, cou, dSPARC, ovarian neoplasm, ovary neoplasm (disease), protein complex, gonada of female reproductive system, tumor of the ovary, Metastases, Proteins, Assay, Ovary, stepk, Ovary Cancers, Cell, Metastase, development, Noradrenaline Bitartrate, MS, Lr, Cuboidal Glandular Epithelial Cells, native protein, natural protein, Protein, IMS, Neoplasm, uterine tube, Cocultivation, MFS1, salpinges, Columnar Glandular Epithelial Cells, l(2)SH0323, Mass Spectrum Analyses, WMS2, tuba uterinae, CYH1, Cancer of the Ovary, Mass Spectrum, mammalian oviduct, ovaries, female organism reproductive system gonada, animal ovary, underdeveloped, Secreted protein acidic and rich in cysteine, female gonad, postnatal growth, ovarium, ovarian epithelial cancer, Glandular Epithelial Cells, OC, fallopian tube, uterine tube (sensu Mammalia), gonad of female organism genitalia, Cancers, Ovarian Neoplasms, Norepinephrine Hydrochloride, dsparc, (R)-, DmelCG11628, ovarian tumor, ON, Protein Gene Products, Gene Proteins, Bioassay, Ovarian Neoplasm, bioassay, ovarian tumours, tumor of the Ovary, Uterine Tubes, neoplasm of the ovary, SSKS, Bra, Co culture, growth, Microenvironments"],"name_synonyms":["grade 3/4, Tube, mammalian oviduct, Mammalian Oviduct, Fallopian tube, Uterine Tube, Moods, female paramesonephric duct, Affects, Mammalian Oviducts, Mammalian, Salpinx, Proteins, Fallopian Tube, secondary metabolites, metabolite, Gene, tuba uterina, fallopian tube, uterine tube (sensu Mammalia), paramesonephric duct of female, Tubes, Oviduct, Uterine, high grade, Fallopian, Protein Gene Products, Salpinges, Gene Proteins, primary metabolites, oviducts, Uterine Tubes, Uterine Salpinx, metabolites, Protein, Oviducts, uterine tube, Gene Products, Mood, malignant ovarian serous tumour, salpinges, serous ovarian cancer., fallopian tubes, salpinx, tuba uterinae, Uterine Salpinges"],"description_synonyms":["moe, CG1849, anon-WO03040301.161, MPS2, human being, Procedures, Laboratory, l(1)G0415, Mus domesticus, leg, Dm NinaC, NINA C, mouse <Mus musculus>, Gene, CG5125, protein, protein-containing complex, House Mouse, Dmoe, Human, nasal sensory epithelium, l(1)AA33, method, Techniques, protein polypeptide chains, CG10701, polypeptide chain, Homo sapiens, House, Method, method used in an experiment, Studies, Gene Products, Mus musculus domesticus, 1, 2, DmelCG10701, NINAC, protein aggregate, gma, Mice, Technique, Man, CG54125, ERM, SIDS, Man (Taxonomy), NinaC, ninac, Swiss, Runt, Swiss Mice, proteins, man, free, CG 5125, T5E21_12., Study, l(1)G0323, anon-WO03040301.155, Nina C, Methodological Studies, l(1)B2/13.1, RUN, Run, D17, anon-WO03040301.157, anon-WO03040301.159, 2.2, house mouse, CT16120, LB5, l(1)LB9, protein complex, T5E21.12, Modern, Proteins, mouse, DmelCG1849, Procedure, Ids, Rnt, results, mice C57BL/6xCBA/CaJ hybrid, AA33, Mus muscaris, DmelCG5125, l(1)G0067, lLB5, native protein, natural protein, Mus, Protein, nasal epithelium, CT42491, pseudostratified main olfactory epithelium, Data Base, DRONINAC, Mus musculus, dMoe, Swiss Mouse, House Mice, l(1)19Ea, Methodological, Methodological Study, human, domesticus, Laboratory Mice, plan specification, Protein Gene Products, P235, MOE, Gene Proteins, Emr1, EMR1, label, Modern Man, l(1)G0404, Mouse, Laboratory Mouse, moesin/ezrin/radixin homolog mRNA"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"Fallopian tube secreted proteins affect ovarian metabolites in high grade serous ovarian cancer","description":"Data files from Q Exactive were imported into Proteome Discoverer (version 2.2, Thermo Fisher) and analyzed using a label-free quantitation method. For MOE PTENshRNA protein IDs, MOE PTENshRNA results were compared to MOE SCRshRNA results against a Mus musculus database (10090_20160912 fasta file) and a contaminants database with a false discovery rate (FDR) at 1% and Percolator. SKOV3 results were run against a Homo sapiens database (9606_20190612 fasta file)and a contaminants database (2015_5 fasta file). Each was analyzed with a FDR at 1% and a Target Decoy. \n","dates":{"publication":"Mon Jul 11 15:52:00 BST 2022"},"accession":"MSV000089866","cross_references":{"pubmed":["36420136"]}}