MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v05/MSV000090908/primaryOK2000000Jim Woodgetthttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=25c3263dc6384e7c92aa45456242625dwoodgett@lunenfeld.caMassIVE255MOD:00685 - "A protein modification that effectively converts an L-glutamine residue to L-glutamic acid."MOD:00684 - "A protein modification that effectively converts an L-asparagine residue to L-aspartic acid."MOD:00719 - "A protein modification that oxygenates an L-methionine residue to one of the diastereomeric L-methionine sulfoxide residues."ProteomicsLTQ Orbitrap EliteHomo Sapiens (ncbitaxon:9606)Lunenfeld Tanenbaum Research InstituteFunctional differentiation of the two isoforms of the protein-serine/threonine kinase, glycogen synthase kinase-3 (GSK-3), is an unsettled area of research. The isoforms are highly similar in structure and are largely redundant, though there is also evidence for specific roles. Identification of isoform-specific protein interactors may elucidate the differences in function and provide insight into isoform-selective regulation. We therefore sought to identify novel GSK-3 interaction partners and to examine differences in the interactomes of the two isoforms using both affinity purification and proximity-dependent biotinylation (BioID) mass spectrometry methods. While the interactomes of the two isomers are highly similar in HEK293 cells, BioID in HeLa cells yielded a variety of preys that are preferentially associated with one of the two isoforms. DCP1B, which favored GSK-3α, and MISP, which favored GSK-3β, were evaluated for reciprocal interactions. The differences in interactions between isoforms may help in understanding the distinct functions and regulation of the two isoforms as well as offer avenues for the development of isoform-specific strategies.Interactomes of Glycogen Synthase Kinase-3 Isoforms.Cormier Kevin W KW, Larsen Brett B, Gingras Anne-Claude AC, Woodgett James R JR293 cell, 293 HEK, GSK-3beta, gsk-3, GSKbeta, DmelCG2621, l(1)G0055, ZW-3, l(1)G0335, zw3(sgg), gsk3-beta, GSK3, Dmsgg3, Gsk-3beta, Gsk3, zw3, ZW3, zw-3, gsk3beta, EG:155E2.3, Cell, Zw3[sgg], anon-WO03040301.254, gsk3, zw[3], l(1)G0263, HeLa Cell, 7330414F15Rik, gsk-3 Gene Product, sgg-zw3, CG2621, human embryonic kidney cell, zw3/sgg, Zw3-sh, GSK3beta, HEK 293, Human Embryonic Kidney 293, DMZ3K25Z, zw3[sgg], l(1)G0183, sgg39, GSK-3betaSgg, GSK-3B, Zw3/GSK3, DMSGG3, GSK3Beta, zw3/GSK3beta, GSK-3, xgsk-3, 293, C86142, HeLa, sgg46, HEK293, SGG/GSK3b, l(1)3Ba, HEK-293, HeLa., Gsk-3, GSK, EG:BACR7C10.8, Dm Zw3, sgg/zw3, Zw-3, GSK3betagr, l(1)zw3, Sgg/Zw3/GSK3, Cells, GSK3beta/SGG, Sgg, SGG, GSK3-beta, sgg10, Zw3, 8430431H08Rik293T Cell, C19orf21, GSK-3beta, 293 Cells, postnatal development., Activity, Laboratory, Human Embryonic Kidney Cell Line 293, L-Threonine, Dmsgg3, growth and development, protein, Social Controls, CDK5/p23, 2-Amino-3-hydroxypropionic acid, Zw3[sgg], protein polypeptide chains, Techniques, Readability, Method, gsk-3 Gene Product, [Tau protein] kinase activity, zw3/sgg, GSK3beta, Kinase, HEK 293, Analysis, Research Activity, protein aggregate, Laboratory Research, Formal Social Controls, Threonin, Priorities, Mass Spectrum Analysis, DMSGG3, functional differentiation, GSK3Beta, Analyses, tau-protein kinase II activity, proteins, purification, procedures, tau-protein kinase I activity, EG:BACR7C10.8, Social, isolation and purification, Biotinylations, Dm Zw3, 2-amino-3-hydroxypropanoic acid, Methodological Studies, TPK II, cdk5/p20, Sgg, SGG, Research Priority, Zw3, tau-tubulin kinase activity, ATP Phosphotransferases, ATP, 293 Cell, GSKbeta, brain protein kinase PK40erk activity, l(1)G0335, zw3(sgg), gsk3-beta, Research Priorities, zw3, ZW3, Procedure, Spectrum Analysis, HEK 293 Cell, Spectroscopy, anon-WO03040301.254, 3-Hydroxyalanine, sgg-zw3, dcp1, tau protein kinase activity, zw3[sgg], sgg39, DCP1, GSK-3betaSgg, Research and Development, Transphosphorylases, Zw3/GSK3, GSK-3, sgg46, Control, Spectrometry, SGG/GSK3b, l(1)3Ba, Methodological, Controls, Gsk-3, Methodological Study, GSK, Activities, HEK 293 Cell Line, HEK 293 Cells, Cells, HEK, GSK3beta/SGG, L Serine, GSK3-beta, L-Serine, Regulation, Glycogen Synthase, Synthase Kinase, Regulations, gsk-3, DmelCG2621, B930050E02Rik, Procedures, conformation, Gene, GSK3, Gsk-3beta, Gsk3, HEK293 Cell, Spectrum Analyses, protein-containing complex, EG:155E2.3, 293T, protein tau kinase activity, gsk3, zw[3], l(1)G0263, polypeptide chain, 7330414F15Rik, isolation, Gene Products, Mass, Studies, Zw3-sh, Phosphotransferase, DMZ3K25Z, Technique, glycogen synthase kinase-3beta activity, Mass Spectroscopy, hDcp1b, tau kinase activity, zw3/GSK3beta, Research, TPK, HeLa, STK31, L Threonine, Transphosphorylase, Study, Glycogen, GSK3betagr, 293T Cells, Development and Research, sgg10, relational structural quality, TPK I, 9130017N09Rik, l(1)G0055, ZW-3, Formal Social Control, protein complex, Proteins, Human Kidney Cell Line 293, function, zw-3, gsk3beta, Phosphotransferases, Cell, development, Serin, Priority, MS, native protein, HeLa Cell, natural protein, Social Control, Protein, Research Activities, CG2621, techniques, l(1)G0183, Mass Spectrum Analyses, GSK-3B, Mass Spectrum, ATP:tau-protein O-phosphotransferase activity, distinct, TTK, xgsk-3, Kinases, C86142, postnatal growth, HEK293, Glycogen Synthase Kinase, Understanding, Protein Gene Products, Gene Proteins, sgg/zw3, Zw-3, l(1)zw3, Sgg/Zw3/GSK3, regulation, growth, 8430431H08Rik, methodologyKinase, Glycogen Synthase Kinase, Glycogen., Glycogen Synthase, Kinases, Synthase KinaseGSK-3beta, gsk-3, GSKbeta, DmelCG2621, l(1)G0055, ZW-3, l(1)G0335, zw3(sgg), gsk3-beta, GSK3, Dmsgg3, Gsk-3beta, Gsk3, zw3, ZW3, zw-3, gsk3beta, EG:155E2.3, Zw3[sgg], anon-WO03040301.254, 8430431H08Rik., gsk3, zw[3], l(1)G0263, 7330414F15Rik, gsk-3 Gene Product, sgg-zw3, CG2621, zw3/sgg, Zw3-sh, GSK3beta, DMZ3K25Z, zw3[sgg], l(1)G0183, sgg39, GSK-3betaSgg, GSK-3B, Zw3/GSK3, DMSGG3, GSK3Beta, zw3/GSK3beta, GSK-3, xgsk-3, C86142, sgg46, SGG/GSK3b, l(1)3Ba, Gsk-3, GSK, EG:BACR7C10.8, Dm Zw3, sgg/zw3, Zw-3, GSK3betagr, l(1)zw3, Sgg/Zw3/GSK3, GSK3beta/SGG, Sgg, SGG, GSK3-beta, sgg10, Zw30PXD038864falseGSK-3 Interactomes from APMS and BioIDInteractomes of GSK-3 Isoforms compared by APMS (in HEK293) and BioID (in HEK293 and HeLa cells).Thu Dec 15 12:37:00 GMT 2022MSV00009090836779422