MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v05/MSV000091408/primaryOK200Lingjun Lihttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=fe2695a96d314b489f3ab7a291154467lingjun.li@wisc.eduMassIVE172MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetProteomicsOrbitrap Fusion LumosHomo Sapiens (ncbitaxon:9606)University of Wisconsin-Madisonrm62, Presenile Alzheimer Dementia, CG10279, scale tissue, ALZHEIMERS DIS, Brp-14, Product, determination, Early Onset, Blood, Silencer factor B, RM62, Mbp1, l(3)s5196, protein, Progress Reports, Dmp68, l(3)rG338, Serum, Dmlip1, Alzheimer's disease, prevention, Proapolipoprotein A-I, Apolipoprotein A1, AD, Polypeptides, protein polypeptide chains, Readability, Apo-AIV, Techniques, unspecified, peptido, Liver activator protein, C4bpa, Method, Biological, Summary Report, Alp-1, Analysis, C|EBP-related protein 2, Biological Product, protein aggregate, Work Flow, myd, prevention and control, Summary Reports, LIP1, INSL3R, imprinted and ancient gene protein, Presenile Alzheimer, Mass Spectrum Analysis, C4BP, Group, LATE ONSET ALZHEIMER DIS, Lips, Biologic Drugs, reference sample, peptides, DAT - Dementia Alzheimer's type, Progress Report, ATP-dependent proteolysis, Analyses, Natural, plant peltate hair, Tissue, LiP, Mbp-1, proteins, Groups, Protein Conformations, Biological Drugs, dmP68, Protein Degradation, Apo-AI, ApoA, preventive measures, Progress, set, GREAT, Biological Medicine, Field Reports, Methodological Studies, AD - Alzheimer's disease, Philtrum, Alzheimer Type Dementia, Great, Medicine, lips, LIP, Lip, ALZHEIMER DIS EARLY ONSET, C/EBPbeta, Tissue Donor, Lvtw-1, Medicines, Presenile, Apolipoprotein A-I(1-242), Biologic Drug, Alzheimers disease, Apo-A, apoA-I, Apoa-4, Senile, Alzheimer disease, ATX, Degradations, preventive therapy, Transplant Donor, sporadic Alzheimer's disease, AI195242, DmRH8, Biologic Products, gyltl1b-b, Acute Confusional Senile Dementia, Apoa-1, apo-AIV, CRP2, Transplant, PRP, familial, oos, lip, Semen Donors, Blood Serum, Procedure, Spectrum Analysis, Alzheimer's Dementia, LIP1Dm, Alzheimer Type, Spectroscopy, Sep-2, Sep-1, Workflows, l(3)01086, Investigative Report, Digestion, MDDGA6, Biopharmaceuticals, mKIAA0609, Biologic Product, NOS, Donors, KIAA0609, l(3)j3D2, l(3)j3D5, Dementia, fg, LGR8, Lgr8, Alzheimers, gyltl1b, Complement, Biological Medicines, Alzheimer, Biological Drug, Protein Digestions, Field, mdc1d, Spectrometry, Control, expanded, Dementias, mC1rA, DLiprin-alpha, Biologics, NF-IL6, Methodological, Methodological Study, Semen Donor, Alzheimer's, Nfil6, Interleukin-6-dependent-binding protein, lip-alpha, MDC1D, Report, Semen, Senile Dementia, enr, Patient, enlarged, apoAIV, Complement System, Alzheimer's Disease, Polypeptide, ApoA-I, 61E3.4, E430016J11Rik, Liver-enriched inhibitory protein, Ovum, Ovum Donors, Organ Donors, Work Flows, Dliprin-alpha, Gruppe, Dementia of the Alzheimer's type, big, Procedures, ProapoA-I, Biologic Pharmaceuticals, peltate hair, Degradation, Liver-enriched transcriptional activator, Gene, apo-AI, DmelCG10279, Spectrum Analyses, protein-containing complex, Focal Onset, Complement Protein, Ximpact, froggy, unspecified (disorder), Gyltl1a, labia oris, TCF-5, ALZHEIMER DIS, method, large, AI132558, LIP.1, dLip1, Conformations, Investigative, polypeptide chain, [X]Dementia in Alzheimer's disease, Transcription factor 5, method used in an experiment, Gene Products, Mass, Studies, p68, C|EBPbeta, CG7279, Technique, Mass Spectroscopy, CG11199, Biologicals, Drugs, Gpr106, apolipoprotein A4, Nuclear factor NF-IL6, apolipoprotein A1, MDDGB6, Natural Product, labeling, RXFPR2, LARGE, Protein Degradations, NF-M, FOCAL ONSET ALZHEIMERS DIS, Transplant Donors, Biological., Study, BPFD#36, Truncated apolipoprotein A-I, grupos, Alzheimer Disease, Philtrums, Clients, great, SF-B, lpr, Control Group, peptidos, Hemolytic, Alzheimer's disease (disorder), Ovum Donor, Investigative Reports, Alzheimers Dementia, LAP, TAG, GPR106, Biologic, Controlled, Organ, Pharmaceuticals, Alzheimer Senile Dementia, DmelCG7279, Products, Disease, Controlling, grupo, protein complex, Biologic Medicines, Proteins, Sep2, Protein Digestion, Digestions, Client, impact-a, Peptide, Primary Senile Degenerative Dementia, group, [X]Dementia in Alzheimer's disease (disorder), MS, native protein, natural protein, Proteolyses, APOA1, APOA4, chemical analysis, Protein, Research Reports, Biopharmaceutical, IMPACT, imprinted and ancient gene protein homolog, Donor, Hemolytic Complement, scales, 4136, Mass Spectrum Analyses, Conformation, covalent modifier, Mass Spectrum, scale, Organ Donor, ensemble, DmelCG11199, Dementia in Alzheimer's disease, lip1, prophylaxis, Rest, Alzheimer's Disease Pathway, Ltw-1, Understanding, LGR8.1, Alzheimer Dementia, DLiprin, Protein Gene Products, plan specification, Drug, Gene Proteins, Complement Proteins, C1r, Late Onset, Reports, C|EBP beta, control, IL-6DBP, Dementia in Alzheimer's disease (disorder), Natural Products, Peptid, assay, ApoA-IV, Summary, groupe, RWDD5, Serums, Field ReportAlzheimer Senile Dementia, Presenile Alzheimer Dementia, Senile, Disease, Alzheimer disease, Degradations, ALZHEIMERS DIS, sporadic Alzheimer's disease, protein complex, Early Onset, Acute Confusional Senile Dementia, Degradation, Proteins, familial, Gene, protein, Spectrum Analyses, Protein Digestion, protein-containing complex, Focal Onset, Dementia of the Alzheimer's type., Digestions, Spectrum Analysis, Alzheimer's disease, Alzheimer's Dementia, unspecified (disorder), Primary Senile Degenerative Dementia, Spectroscopy, Alzheimer Type, [X]Dementia in Alzheimer's disease (disorder), ALZHEIMER DIS, AD, protein polypeptide chains, unspecified, MS, native protein, Proteolyses, natural protein, polypeptide chain, [X]Dementia in Alzheimer's disease, Digestion, Protein, Mass, Gene Products, NOS, Analysis, protein aggregate, Mass Spectrum Analyses, Mass Spectroscopy, Dementia, Mass Spectrum Analysis, Presenile Alzheimer, Mass Spectrum, LATE ONSET ALZHEIMER DIS, Alzheimers, ATP-dependent proteolysis, Analyses, DAT - Dementia Alzheimer's type, Dementia in Alzheimer's disease, Alzheimer, Protein Digestions, Spectrometry, Alzheimer's Disease Pathway, Dementias, labeling, proteins, Protein Degradations, Alzheimer Dementia, Protein Degradation, Alzheimer's, FOCAL ONSET ALZHEIMERS DIS, Protein Gene Products, Gene Proteins, Senile Dementia, Late Onset, AD - Alzheimer's disease, Alzheimer Disease, Alzheimer Type Dementia, Dementia in Alzheimer's disease (disorder), ALZHEIMER DIS EARLY ONSET, Alzheimer's Disease, Alzheimer's disease (disorder), Presenile, Alzheimers Dementia, Alzheimers diseasePXD040585falseDiLeu isobaric labeling coupled with limited proteolysis mass spectrometry for high-throughput profiling of protein structural changes in Alzheimers diseaseHigh-throughput quantitative analysis of protein conformational changes has a profound impact on our understanding of the pathological mechanisms of Alzheimers disease (AD). To establish an effective workflow enabling quantitative analysis of changes in protein conformation within multiple samples simultaneously, here we report the combination of N,N-dimethyl leucine (DiLeu) isobaric tag labeling with limited-proteolysis mass spectrometry (DiLeu-LiP-MS) for high-throughput structural protein quantitation in serum samples collected from AD patients and control donors. 23 proteins were discovered to undergo structural changes, mapping to 35 unique conformotypic peptides with significant changes between the AD group and the control group. 7 out of 23 proteins, including CO3, CO9, C4BPA, APOA1, APOA4, C1R and APOA, exhibited potential correlation with AD. Moreover, we found that complement proteins (e.g., CO3, CO9 and C4BPA) related to AD exhibited elevated levels in the AD group compared to those in the control group. These results provide evidence that the established DiLeu-LiP-MS method can be used for high-throughput structural protein quantitation, which also showed great potential in achieving large-scale and in-depth quantitative analysis of protein conformational changes in other biological systems. Fri Mar 03 12:33:00 GMT 2023MSV000091408