MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v05/MSV000091953/primaryOK2000000Xiao-Bo Qiuhttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=0ad8e2db0b454223ad7682a03a20ccccxqiu@bnu.edu.cnMassIVE13UNIMOD:903 - "Lys->Cys substitution and carbamidomethylation."UNIMOD:1 - "Acetylation."UNIMOD:35 - "Oxidation or Hydroxylation."ProteomicsQ Exactive PlusHomo Sapiens (ncbitaxon:9606)Beijing Normal UniversityA430040A19Rik, IL1BC, T22F8.160, Meth, and rna binding 2, Chronic Airflow Obstructions, CASP-1, CG7788, crice, A430032G04Rik, chronic obstructive pulmonary disease (COPD), CT6637, mg/ml, T22F8_160, APOLLON, ATGPR7, chronic obstructive airways disease NOS (disorder), Chronic irreversible airway obstruction, DGS, SDS-PAGE, GRP8, GRP7, Analysis, Chronic Obstructive Pulmonary Disease (COPD), Il1bc, rounded, Mass Spectrum Analysis, COLD (chronic obstructive lung disease), Polyacrylamide, SDS-PAGE electrophoresis, Analyses, NEC in ICD9CM_2006, CAFL - Chronic airflow limitation, IBP, IL-1BC, Chronic airflow limitation, CT6660, ICE, Ice, disease (COPD), GLYCINE RICH PROTEIN 7, DmelCG1106, anatomical tube, sample, Bladder pain syndrome, glycine-rich RNA-binding protein 8, CHRONIC OBSTRUCTIVE PULMONARY DISEASE, CHRONIC, Tube, chronic obstructive lung disease [Ambiguous], obstructive lung disease, Caspase-1 subunit p10, BZRP, iCE, ice, drice, Spectrum Analysis, Airflow Obstructions, DmelCG7788, PBS, PBR, Spectroscopy, CHRONIC OBSTRUCTIVE AIRWAY DIS, pulmonary disease (COPD), TUBE, drIce, drICE, 1-L-aspartic acid-2-L-tyrosine-3-L-lysine-, CHRONIC OBSTRUCTIVE, chronic obstructive pulmonary disease and allied conditions, Chronic airway disease, NOS, Airflow Obstruction, COPD NOS, Crystal, CHRONIC OBSTRUCTIVE LUNG DIS, GLYCINE-RICH PROTEIN 8, Chronic Obstructive Airways Disease, Chronic airway obstruction, Chronic obstructive lung disease, absence of, Painful Bladder Syndrome, cold, Sodium Dodecyl Sulfate-PAGE, Sonications, DPKN, P45, Spectrometry, SDS polyacrylamide gel electrophoresis, CG1106, Speed, dPKN, Chronic Airflow Obstruction, Painful bladder syndrome, F2G1.4, OBSTRUCTIVE PULMONARY DISEASE (COPD), COPD - Chronic obstructive pulmonary disease, PRK2, duct, Drice, chronic obstructive airways disease, BRUCE, p45, Bruce, obstructive pulmonary disease (COPD), Sodium Dodecyl Sulfate-PAGEs, DRICE, chronic obstructive lung disease, COLD, Bladder Pain Syndrome, BPBS, DYKDDDDK peptide, Pk?3, DISEASE (COPD), DrIce, DrICE, CAO - Chronic airflow obstruction, Gene, mKIAA1289, Gel Electrophoresis, l(2)45Ca, circadian rhythm, CHRONIC OBSTRUCTIVE PULM DIS, Spectrum Analyses, 3-2, Chronic obstructive pulmonary disease NOS, abdominal muscles, SDS polyacrylamide gel electrophoresis of proteins, Chalk, and RNA binding 1, Buffer, Chronic obstructive pulmonary disease finding, Cold, Sodium Dodecyl Sulfate PAGE, PULMONARY DISEASE (COPD), Pulmonary Disease, Trypsinogen (human pancreas isoenzyme 2 activation peptide), Filtrations, Tina, Polyacrylamide Gel Electrophoresis, Dpkn, Gene Products, Mass, l(2)06736, Mass Spectroscopy, Cold Temperatures, anatomical systems, DmelCG2049, Glass, 2310061K05Rik, gel, PCE-2, pk18, 2-1, 2-2, Pk45C., Chronic Obstructive, MBR, v(2)rG232, lysate, Caspase-1 subunit p20, ATGRP7, ATGRP8, NEC, PTBR, Chronic, SDS PAGE, COPD, COAD - Chronic obstructive airways disease, chronic obstructive airways disease NOS, DBI, caspase 3, not elsewhere classified, COAD, Proteins, CG2055, CE-2, COLD - Chronic obstructive lung disease, buffer, Cell, chronic, PULM DIS CHRONIC OBSTRUCTIVE, Chronic obstructive pulmonary disease finding (finding), MS, Temperatures, SPR-2A, PKBS, Protein, AI551343, PKN, CES2A1, GLYCINE-RICH RNA-BINDING PROTEIN 7, (COPD), chronic airway obstruction, eagle-Barrett syndrome, chronic obstructive, dJ142L7.2, CAL - Chronic airflow limitation, Mass Spectrum Analyses, chronic obstructive airway disease, Temperature, Mass Spectrum, with urinary tract Abnormality and cryptorchidism, Dops, sodium dodecyl sulphate–polyacrylamide gel electrophoresis, GR-RBP7, GR-RBP8, IL-1 beta-converting enzyme, Episome, Interleukin-1 beta-converting enzyme, mDRC, D630005A10Rik, sample population, Interleukin-1 beta convertase, Protein Gene Products, Episomes, Gene Proteins, chronic obstructive lung disease (disorder), 3.4.22.36, Plasmid, cold (chronic obstructive lung disease), CG2049, round, Chronic Obstructive Lung Disease, AA501170, RCB1375, chronic obstructive pulmonary diseaseBruce, A430040A19Rik, A430032G04Rik, mKIAA1289, AA501170, APOLLON, BRUCE., D630005A10Rik0PXD042246falseMS for BIRC6 Complex from HEK293F The plasmids were then transiently transfected into HEK293F expression system for overexpression. After incubation for 48 h, the cells were collected, washed one with ice-cold PBS. The resuspended cells were then lysed by sonication, and the lysate supernatant after centrifugation was incubated with M2-FLAG affinity beads for 1.5 h. After several round of washing with buffer B, the FLAG-affinity proteins were eluted by 0.5 mg/mL FLAG peptide dissolved in buffer C. The elute was further purified by gel filtration (Superose 6 Increase Columns, Cytiva) using buffer C. The fraction at 0.5 mL/tube was collected starting from 7 mL. Concentrate a few tubes from the main peak diagram. The purified FLAG-BIRC6 complex was analyzed by mass spectrometry following separation of the complex by SDS-PAGE. The full lane was sliced into 6 pieces from top to bottom with assigned sample numbers: 1, 2-1, 2-2, 3-1, 3-2, and 4.Mon May 15 17:16:00 BST 2023MSV000091953