MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v05/MSV000092123/primary200OKProteomicsSandeep MallipattuQ Exactive HF Hybrid Quadrupole-OrbitrapMus Musculus (ncbitaxon:10090)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=08d1896c2de340fca8cf078d9549aa01sandeep.mallipattu@stonybrookmedicine.eduStony Brook UniversityMassIVE51No PTMs are included in the datasetSoluble, Insulin B Chain, Tandem, Laboratory, doxycycline, Globulin, Blood, Mus domesticus, HLB., Vibramycin Novum, insuline humaine, CASP-14, Serum, Mass Spectrometry Mass Spectrometry, House Mouse, BU-3839T, BU 3839T, 10, 11, 12, Glomerular, Liver regeneration-related protein LRRG03, Doxycycline Chinoin, human insulin, 1, Phenol, 2, Vibra-Tabs, Min, 4, 5, 6, DmelCG42273, (R*, Carbolic Acid, beta 2 Transferrin, insulin, acide carbolique, Selenium 80, hypoplasia, min, selenium, Swiss Mice, Karbolsaeure, mAPC, Mass Spectrometry, IBP, 11-DIOXO-1, AI047805, Phenylic acid, SRN1, doxycyclinum, insulin human, podocin, Sputolysin, Carbol, Podocyte, RED, Red, Se, idiopathic, house mouse, steroid-resistant, Bladder pain syndrome, Phenylic alcohol, Phenic acid, insulinum humanum, Transferrin, 34Se, 12a-octahydro-3, Regular, Doxycycline Monohydrate, Periostat, BZRP, 4A, tau Transferrin, mouse, Dmel_CG7826, beta-1, Glomerular Visceral, 4-dimercapto-2, Doxycycline Phosphate (1:1), Blood Serum, BMY-28689, HMG-CoAR, CSA2, 5-hydroxy-alpha-6-deoxytetracycline, PBS, Regular Insulin, PBR, acide phenique, Isotransferrin, Selen, insulin (recombinant), 5aalpha, Dm1, Cleland, 5A, 4a, Mini-ICE, tau-Transferrin, Soluble Insulin, 6alpha-deoxy-5-oxytetracycline, Dmel_CG7835, autosomal recessive, 5S, Mnb, MNB, Mus musculus, Phenolate, absence of, Painful Bladder Syndrome, beta-1 Metal-Binding Globulin, Vibra Tabs, (4S, Caspase-14 subunit p10, Vibramycin, MS/MS, 5a, mice, Swiss Mouse, carbolic acid, insulina humana, Transferrins, beta 1 Metal Binding Globulin, Transferrin C, Transferrin B, Caspase-14 subunit p19, 3-Butanediol, AW124434, MICE, beta Trypsin, 6R, Metal-Binding Globulin, domesticus, R*)-1, Sodium Salt, BU3839T, Painful bladder syndrome, 4-(dimethylamino)-1, label, Acetamide, Glomerular Visceral Epithelial Cells, Mouse, Serotransferrin, 12AS)-4-(DIMETHYLAMINO)-3, 4-Dimercapto-2, Doxycycline Monohydrochloride, Bladder Pain Syndrome, BPBS, Cleland Reagent, 3.4.22.-, 4aalpha, BMY28689, rac-Dithiothreitol, Doxycycline Calcium, threo-1, Reagent, nephrotic syndrome, selenio, Cleland's reagent, Supracyclin, Doxycycline-Chinoin, 2-Naphthacenecarboxamide, 4-dimercapto-, mini-ICE, Insulin B, 12A-PENTAHYDROXY-6-METHYL-1, R*)-, abdominal muscles, Monoferric Transferrins, CG7826, Hydramycin, PRO1400, 5AR, AI790225, reduced, House, Insulin, Sodium Phenolate, Alpha-6-Deoxyoxytetracycline, CG7835, Mus musculus domesticus, CG42273, Selenium-80, tiny, Doxycycline Calcium Salt (1:2), Mice, Chain, hYAK3-2, 6alpha, Benzenol, Swiss, MS2, Siderophilin, Doxycycline Hyclate, beta-Trypsin, pk18, MBR, Alpha 6 Deoxyoxytetracycline, Iletin, 4AR, (4S-(4alpha, Insulin recombinant, Epithelial Cells, Jenacyclin, PTBR, Oxybenzene, Doryx, Monoferric, PDCN, 12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE, 2-iodo-, DYRK5, small, 3-butanediol, Oracea, DYRK1, doxiciclina, Trf, DBI, Doxycycline Hemiethanolate, Vibravenos, Clelands Reagent, Hydroxybenzene, Selenium, Atridox, Cell, Dithiothreitol, BMY 28689, Dithiotreitol, Beta-1 metal-binding globulin, Sodium Insulin, PKBS, Mus, Insulin A Chain, DL-threo-1, beta 2-Transferrin, 5alpha, Sodium, Novolin, Dyrk1, eagle-Barrett syndrome, tandem MS, DTL, Cleland's, AU020952, DTT, with urinary tract Abnormality and cryptorchidism, Exubera, PRSS, underdeveloped, 12aalpha))-, Dihydrate, CC1, Visceral Epithelial Cells, 11-dioxo-, Tripcellim, PhOH, House Mice, 12a-pentahydroxy-6-methyl-1, doxycycline (anhydrous), Mass Spectrometry-Mass Spectrometry, mDRC, REDK, 6 epimer, 6-epimer, beta-1 Metal-Binding, Laboratory Mice, ME-IV, Cleland's Reagent, Trypure, Doxycyclin, Carbolsaeure, Phenolate Sodium, NPHS2, Laboratory Mouse, SerumsPeptidomics., urinary levels, urinary aspectsfalseKLF6PODTA-Conditioned media and urine proteomicsTandem mass spectrometry was performed on the supernatant from mouse primary podocytes isolated from KLF6PODTA and NPHS2-rtTA mice (with and without doxycycline) using a label-free proteomic approach. After the cells reached confluency, cells were washed five times with PBS and medium was replaced with phenol-red, insulin-transferrin-selenium (ITS), and serum-free RPMI. After 48 additional hours, supernatant (conditioned media) was harvested and centrifuged for 5 min at 500 g and then for 10 min at 1500 g. Samples were reduced (dithiothreitol), alkylated (iodoacetamide), digested with trypsin, and cleaned using hydrophobic-lypophilic-balance (HLB) pack C-18. Wed Jun 07 08:50:00 BST 2023MSV000092123