{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v06/MSV000092538/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Jin Huk Choi"],"instrument_platform":["timsTOF Pro"],"species":["Mus Musculus (ncbitaxon:10090)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=f9a5290cb1e743d9a9465f29fc00bc89"],"submitter_email":["Jin.Choi@utsouthwestern.edu"],"submitter_affiliation":["University of Texas Southwestern Medical Center"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["2,981"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"description_synonyms":["Laboratory, Mus domesticus, N-Ethyl-N-nitroso-urea, CASP-14, protein, sci, Medullary, House Mouse, Spleen, Medullary Hematopoiesis, Mutations, protein polypeptide chains, GTPases, medulla ossea, symptoms, Urea, HOW, How, cytopathology, N Ethyl N nitrosourea, protein aggregate, Lymphocytopenias, l(3)j5D5, Decreased blood lymphocyte number, 24B, average, Lymphopenias, Immune Processes, Immune Responses, AI327394, Triphosphate Phosphohydrolases, 1-Ethyl-1-nitrosourea, stru, iecur, Swiss Mice, proteins, l(3)S053606, inhibition of homeostatic process, RGD1305751, CG10293, ecotype, Red Marrow, l(3)j5B5, Immune, blood cell formation, D630024P16, Pathologies, Red, homeostasis, Allele, Ethyl nitrosourea, Strains, associated, lyp, screening, 0904/17, NCU-G1, Guanosine, Process, GTP Phosphohydrolase, frequency, 2010110D23Rik, mouse, Hematopoiesis, Ian4l1, AI227039, E230026N22Rik, IAN4, Ian4, Ian5, gtl11, N-Ethylnitrosourea, Low lymphocyte number, positive regulation of homeostatic process, Absolute lymphocyte count decrease, lymphocytopenia, SZ1, Mini-ICE, Lipid, cultivar, Large spleen, Mus musculus, N-ethyl-N-nitroso-, histopathology, Caspase-14 subunit p10, medulla of bone, ENU, mice, Swiss Mouse, signs, Caspase-14 subunit p19, MICE, surveillance, morbidity, Lymphocytopenia, RGD1303130, domesticus, SLC Proteins, Enlarged, Phosphohydrolase, anon-EST:Liang-2.39, Mouse, mKIAA1478, Strains and Sprains, Bone, Aethylnitroso-harnstoff, GTP, 3.4.22.-, lipids, Allelomorphs, Carrier Protein, lysosome membrane, Enlarged Spleen, SLC, N-Ethyl-N-nitroso carbamide, P62, Gene, mini-ICE, haemopoiesis, protein-containing complex, Guanosine Triphosphate, Carrier Proteins, jecur, polypeptide chain, House, Marrow, Gene Products, Solute Carrier, Mus musculus domesticus, Increased spleen size, lymphocyte cell differentiation, Yellow, Mice, Sprains, lymphopenia (disease), Guanosine Triphosphate Phosphohydrolases, study, l(3)s2612, occurrence, Swiss, prevalence, Allelomorph, Immune Response, DmelCG10293, AB027141, negative regulation of homeostatic process, 0610031J06Rik, Band25, Immune Process, Gimap5, Solute Carrier Protein, GTPase, incidence, lymphopenia, NEU, Autoregulation, findings, Guanosinetriphosphatases, protein complex, clone 2.39, Proteins, Sprain, 1200003O06Rik, function, qkr, l(3)S090417, regulation of homeostatic process., strain, native protein, natural protein, medulla ossium, Mus, KH93F, Protein, proteomic analysis, Strain, regulation of homeostatic process, outbreaks, who, MgcRacGAP, Yellow Marrow, Nitrosoethylurea, lymphocytic blood cell differentiation, Solute, lymphocyte development, medullary bone, hematopoiesis, House Mice, Haematopoiesis, Who/How, 0610031j06rik, endemics, 1-(Aminocarbonyl)-1-ethyl-2-oxohydrazine, Laboratory Mice, N-Ethyl-N-nitrosourea, Protein Gene Products, Phosphohydrolases, Livers, Gene Proteins, activation of homeostatic process, blood cell biosynthesis, Response, qkr[93F], epidemics, biopsy, Laboratory Mouse, SMAP4"],"name_synonyms":["Autoregulation, lyp, NCU-G1, death rate, 2010110D23Rik, iecur, 1200003O06Rik, Ian4l1, inhibition of homeostatic process, 0610031j06rik, E230026N22Rik, RGD1305751, IAN4, Ian4, Ian5, Cell, regulation of homeostatic process., RGD1303130, Concept, jecur, Livers, activation of homeostatic process, Role Concept, survival, D630024P16, positive regulation of homeostatic process, Roles, time of survival, Role Concepts, Cells, Role, AB027141, negative regulation of homeostatic process, Concepts, 0610031J06Rik, homeostasis, Lymphoid, Lymphoid Cell, Gimap5, Lymphocyte, Lymphoid Cells, SMAP4"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"Essential role of MFSD1-GLMP-GIMAP5 in lymphocyte survival and  liver homeostasis","description":"In this study, we detected three alleles that caused lymphopenia, splenomegaly, progressive liver pathology and extramedullary hematopoiesis (EMH), resulting from N-ethyl-N-nitrosourea (ENU)-induced mutations in the major facilitator superfamily domain containing 1 protein (Mfsd1) which encodes a lysosomal membrane-bound solute carrier protein with no previously described function in immunity. By proteomic analysis, we identified MFSD1 functions together with GLMP (glycosylated lysosomal membrane protein), and GIMAP5 (GTPase of immunity-associated protein 5). Germ line knock alleles of MFSD1, GLMP, and GIMAP5 were generated in mice and confirmed occurrence of lymphopenia, liver pathology, EMH, and lipid deposition in the bone marrow and liver of each strain. We found that the interaction of MFSD1 and GLMP with GIMAP5 is essential to maintain normal GIMAP5 expression that is critical to support lymphocyte development and liver homeostasis that suppresses EMH. These findings provide new insight into the mechanism of how MFSD1-GLMP-GIMAP5 complex functions together to regulate immunity and liver homeostasis that were previously unknown.","dates":{"publication":"Thu Jul 27 10:57:00 BST 2023"},"accession":"MSV000092538","cross_references":{}}