{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v06/MSV000092738/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Adam L. Hollerbach"],"instrument_platform":["Q-Exactive Plus with 11-meter SLIM"],"species":["Peptide Standards"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=f5f55ec9b3d3460ea0db802c1aae30fd"],"submitter_email":["Adam.Hollerbach@pnnl.gov"],"submitter_affiliation":["Pacific Northwest National Laboratory"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["4"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"pubmed_abstract":["High-resolution ion mobility spectrometry-mass spectrometry (HR-IMS-MS) instruments have enormously advanced the ability to characterize complex biological mixtures. Unfortunately, HR-IMS and HR-MS measurements are typically performed independently due to mismatches in analysis time scales. Here, we overcome this limitation by using a dual-gated ion injection approach to couple an 11 m path length structures for lossless ion manipulations (SLIM) module to a Q-Exactive Plus Orbitrap MS platform. The dual-gate setup was implemented by placing one ion gate before the SLIM module and a second ion gate after the module. The dual-gated ion injection approach allowed the new SLIM-Orbitrap platform to simultaneously perform an 11 m SLIM separation, Orbitrap mass analysis using the highest selectable mass resolution setting (up to 140 k), and high-energy collision-induced dissociation (HCD) in ∼25 min over an <i>m/z</i> range of ∼1500 amu. The SLIM-Orbitrap platform was initially characterized using a mixture of standard phosphazene cations and demonstrated an average SLIM CCS resolving power (Rp<sup>CCS</sup>) of ∼218 and an SLIM peak capacity of ∼156, while simultaneously obtaining high mass resolutions. SLIM-Orbitrap analysis with fragmentation was then performed on mixtures of standard peptides and two reverse peptides (SDGRG<sup>1+</sup>, GRGDS<sup>1+</sup>, and Rp<sup>CCS</sup> = 305) to demonstrate the utility of combined HR-IMS-MS/MS measurements for peptide identification. Our new HR-IMS-MS/MS capability was further demonstrated by analyzing a complex lipid mixture and showcasing SLIM separations on isobaric lipids. This new SLIM-Orbitrap platform demonstrates a critical new capability for proteomics and lipidomics applications, and the high-resolution multimodal data obtained using this system establish the foundation for reference-free identification of unknown ion structures."],"pubmed_title":["A Dual-Gated Structures for Lossless Ion Manipulations-Ion Mobility Orbitrap Mass Spectrometry Platform for Combined Ultra-High-Resolution Molecular Analysis."],"pubmed_authors":["Hollerbach Adam L AL, Ibrahim Yehia M YM, Meras Vanessa V, Norheim Randolph V RV, Huntley Adam P AP, Anderson Gordon A GA, Metz Thomas O TO, Ewing Robert G RG, Smith Richard D RD"],"pubmed_title_synonyms":["Mass Spectrum Analysis, Spectroscopy, Mass Spectrum, MS, Ionen, ion, Ion, Analyses, determination, iones, Mass, Spectrometry, chemical analysis., assay, Analysis, Spectrum Analyses, ions, Mass Spectrum Analyses, Spectrum Analysis, Mass Spectroscopy"],"description_synonyms":["Water, Tetradecapeptide, Bru, Angiotensin III, Angiotensin 3-8, MeCN, Raw, NCMe, FLH1A, Vinegar, Large neuromedin N, H(2)O, Isoleucine(5)-, 5 L Isoleucine Angiotensin II, BOUND WATER, Essigsaeure, oxidane, Bradykinin Diacetate, Bradykinin Acetate, Long Term, WATER, HOH, SLIM1, Polypeptides, 5-L-Isoleucine, NKA, Ang III, E260, peptido, Tail peptide, Glacial Acetic Acid, Neurotensin, CH3-C#N, GRP1, Grp1, XMPMA, Kallidin II, Angiotensin 1-10, Effect, Fibrinogen alpha chain, Svc, Hypertensinogen, Hypothalamic, peptides, BK, L-Arg-L-Pro-L-Pro-Gly-L-Phe-L-Ser-L-Pro-L-Phe-L-Arg, NmN, PTPSTEP, SLIM-1, Acetic Acid, PPT, Neuromedin L, T6G21.3, Acetic Acid Glacial, Arg Pro Pro Gly Phe Ser Pro Phe Arg, (1-D-Arg)-Isomer, Angiotensin-1, Neuromedin N, (2-D-Pro)-Isomer, Angiotensin-3, Angiotensin-2, Angiotensin-4, l(2)k08110, sample, MeCO2H, Ang II, High molecular weight kininogen, dihydridooxygen, ethoic acid, dihydrogen oxide, Renin Substrate Tetradecapeptide, Ang IV, Long-Term Effects, GPH, FHL1A, Stars, FHL1B, Angiotensin 1-9, Angiotensin 1-8, Angiotensin 1-7, SERPINA8, Angiotensin 1-5, Angiotensin 1-4, aqua, Longterm Effect, Euler-Gaddum Substance P, Euler Gaddum Substance P, Neurokinin A, Des-Asp[1]-angiotensin II, Fibrinopeptides A, H2O, Ang I, Glacial, CG5186, (5-D-Phe-8-D-Phe)-Isomer, Neuropeptide gamma, 3.1.3.48, 4732462F18Rik, HMWK, (3-D-Pro)-Isomer, (2-D-Pro-7-D-Pro)-Isomer, Lysyl-bradykinin, Hydrogen Oxide, Angiotensin 2-8, NPK, Ethylic acid, Angiotensin IV, Step, CG11628, leucyl-arginyl-arginyl-alanyl-seryl-leucyl-glycine, angiotensina II, (7-D-Pro)-Isomer, hydrogen hydroxide, 5-L-Isoleucine Angiotensin II, Hypothalamic Substance P, Low molecular weight growth-promoting factor, ANG-(1-8)Octapeptide, Striatum-enriched protein-tyrosine phosphatase, Renin Substrate, Euler-Gaddum, Angiotensin II, acqua, l(2)s1866, ang II, RBP-associated molecule 14-1, Ile(5)-, Melittin (honeybee), Angiotensin-(1-8) Octapeptide, STEP, Kallidin I, Neuropeptide K, Polypeptide, Wasser, methyl cyanide, Angiotensinogen (protein renin substrate), (2-D-Pro-3-D-Pro-7-D-Pro)-Isomer, STARS, RAM14-1, RPPGFSPFR, Fibrinopeptide A, Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, Williams-Fitzgerald-Flaujeac factor, E-260, Renin-Substrate, slim, GRP1/cytohesin 1, Effects, Fitzgerald factor, C-terminal-flanking peptide, angiotensinum II, FHL-1, Kininogen-1 heavy chain, CH3-COOH, 1-8-angiotensin I, SLIM, agua, SP(1-11), CG11633, cytohesin/GRP1, SLIMMER, Methanecarboxylic acid, Bradykinin Triacetate, L-bradykinin, Ms1, NmN-125, Del(8)44H, Mellitin, CH3CO2H, Proangiotensin, Ile-Ser-Bradykinin, MS1, Longterm, l(2)SH2 0323, Bradykinin, T-kinin, eau, Long-Term, Isoleucine(5)-Angiotensin, Substance K, (6-D-Ser)-Isomer, Serpin A8, Neural-specific protein-tyrosine phosphatase, (9-D-Arg)-Isomer, peptidos, Long-Term Effect, Bradykinin Hydrochloride, Slim, (8-D-Phe)-Isomer, Melittin, AcOH, Kininogen-1 light chain, (3-D-Pro-7-D-Pro)-Isomer, 1-(14)C-labeled, 3H-labeled, Val(5)-, stepk, Skeletal muscle LIM-protein 1, Mischung, acetonitrile, male sterility 1., Peptide, Substance P, HOAc, water, Long Term Effects, Valyl(5)-Angiotensin II, Isoleucyl(5)-Angiotensin II, l(2)SH0323, ACETONITRILE, cyanomethane, Alpha-2-thiol proteinase inhibitor, NN, CYH1, NT, Col4a-1, E 260, MALE STERILITY 1 PROTEIN, (5-D-Phe)-Isomer, Striated muscle activator of Rho-dependent signaling, acide acetique, ethanenitrile, sample population, Longterm Effects, DmelCG11628, Angiotensin I, MeCOOH, concentration, Leu-Arg-Arg-Ala-Ser-Leu-Gly, Valine(5)-, KYOT, KyoT, Peptid, INS No. 260, [OH2], DmelCG5186, Ethanoic acid"],"pubmed_abstract_synonyms":["Kation, Psychological Power, lipids, scale tissue, slim, ion, determination, Effects, Professional Power, Peptidomics, FLH1A, FON1, Lipidomic, cation, FHL-1, FBN, SUPERMAN, Spectrum Analyses, Measure, FLORAL ORGAN NUMBER 1, thomson, Foundation, SLIM, heavy chain disease, Long Term, CG7826, mass-to-charge ratio, SLIM1, peptide, SLIMMER, Polypeptides, Personal, DmelCG3424, Differential Mobility Analysis, peptido, Injection, ECTOL1, SUP, Mass, CG7835, CG42273, XMPMA, Min, Analysis, violaxanthin-capsorubin isomerase (ketone-forming) activity, Effect, Injectables, anon-WO0118547.370, WMS, Mass Spectroscopy, DmelCG42273, Mass Spectrum Analysis, Psychological, average, iones., capsanthin-capsorubin synthase activity, GRGDS, anatomical systems, peptides, Analyses, Longterm, iones, MS2, Bdr, SLIM-1, min, Weights, Measures, mAPC, Long-Term, Super protein, ions, free, sp, SNAP-25, OCTD, AI047805, Social, Injectable, cations, Mif1, s, Cation, peptidos, Long-Term Effect, u, Social Power, GPHYSD2, Slim, Long-Term Effects, SGS, Power, DYRK1, FHL1A, FHL1B, high mass, Kationen, Longterm Effect, CG3424, Dmel_CG7826, Lipidomes, Th, unified atomic mass unit, Skeletal muscle LIM-protein 1, Synaptosomal-associated 25 kDa protein, Mischung, Psychological Powers, large mass, Spectrum Analysis, collisionally activated dissociation, Da, Peptide, cationes, CID, ACMICD, Spectroscopy, polypeptide, MS, ketoxanthophyll synthase activity, Gly-Arg-Gly-Asp-Ser, CAD, CG5186, HCD, Ionen, Dm1, IMS, chemical analysis, Long Term Effects, Lipid, Ion Mobility, GENA70, MFS1, Professional, Dyrk1, scales, 4732462F18Rik, Differential Mobility, Mass Spectrum Analyses, Dmel_CG7835, tandem MS, WMS2, Mnb, MNB, AU020952, Mass Spectrum, amu, Scales, MS/MS, CC1, Spectrometry, FLO10, MASS, AW124434, HERP, Longterm Effects, ME-IV, l(2)s1866, Personal Power, Lipidome, pth, RBP-associated molecule 14-1, capsanthin/capsorubin synthase activity, Ion, SNAP, SSKS, Power (Psychology), KYOT, KyoT, CCS, Peptid, Measures and Weights, assay, Polypeptide, Powers, anon-WO0118547.271, RAM14-1, DmelCG5186, FLORAL DEFECTIVE 10"],"name_synonyms":["FHL1A, FHL1B, slim, peptides, FLH1A, DmelCG5186., FHL-1, SLIM-1, Skeletal muscle LIM-protein 1, SLIM, not genetically inherited, Peptide, l(2)s1866, SLIM1, polypeptide, peptide, Polypeptides, SLIMMER, RBP-associated molecule 14-1, peptido, CG5186, KYOT, KyoT, Peptid, peptidos, XMPMA, Polypeptide, 4732462F18Rik, Slim, RAM14-1"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"Peptide standard acquired with the dual-gated SLIM-Orbitrap","description":"Raw data collected on the dual-gated SLIM-Orbitrap. The sample contained a mixture of standard peptides at 1 uM equimolar concentration in 60:40 water:acetonitrile with 1% acetic acid (v/v). The peptides were kemptide, angiotensin I, bradykinin, neurotensin, angiotensin II, renin substrate tetradecapeptide procine, melittin, substance P, and fibrinopeptide A. Acquisition parameters included: starting arrival time of 86 ms, 1 ms / step, width of 1 ms, 5 scans per step, MS1 only.","dates":{"publication":"Wed Aug 23 17:08:00 BST 2023"},"accession":"MSV000092738","cross_references":{"pubmed":["37307303"]}}