MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v06/MSV000092898/primary200OK0000ProteomicsOliver BaarsOrbitrap Exploris 480Pyrenophora Biseptata (ncbitaxon:2211333)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=b3a2abbb5b52442686b1bcbef34fd4c5obaars@ncsu.eduNorth Carolina State UniversityMassIVE14MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetWe investigated the ability of four plant and soil-associated fungi to modify or degrade siderophore structures leading to reduced siderophore iron-affinity in iron-limited and iron-replete cultures. Pyrenophora biseptata, a melanized fungus from wheat roots, was effective in inactivating siderophore iron-chelating moieties. In the supernatant solution, the tris-hydroxamate siderophore desferrioxamine B (DFOB) underwent a stepwise reduction of the three hydroxamate groups in DFOB to amides leading to a progressive loss in iron affinity. A mechanism is suggested based on the formation of transient ferrous iron followed by reduction of the siderophore hydroxamate groups during fungal high-affinity reductive iron uptake. P. biseptata also produced its own tris-hydroxamate siderophores (neocoprogen I and II, coprogen and dimerum acid) in iron-limited media and we observed loss of hydroxamate chelating groups during incubation in a manner analogous to DFOB. A redox-based reaction was also involved with the tris-catecholate siderophore protochelin in which oxidation of the catechol groups to quinones was observed. The new siderophore inactivating activity of the wheat symbiont P. biseptata is potentially widespread among fungi with implications for the availability of iron to plants and the surrounding microbiome in siderophore-rich environments.Inactivation of siderophore iron-chelating moieties by the fungal wheat root symbiont Pyrenophora biseptata.French Katie S KS, Chukwuma Emmanuel E, Linshitz Ilan I, Namba Kosuke K, Duckworth Owen W OW, Cubeta Marc A MA, Baars Oliver ODeferoxamin, Microbial Community Composition, Activity, DFO, Pflanze, 5-piperazinediyl)bis(trimethylene))bis(5-hydroxy-3-methyl-2-pentenamide), Methanesulfonate, biosynthesis, Brachysporium biseptatum, Amide, Triticum aestivum subsp. aestivum, Environmental., Migrant Workers, viridiplantae, Human, Desferioximine, Background, reduced, Peat, Cultural, CG9063, 1, 2-hydroxyphenol, Impacts, 26Fe, Desferrioxamine B Mesylate, tiny, Environmental Impacts, Desferrioxamine B, Wheat, multicellular organismal biosynthetic process, plantae, Pilz, Filamentous Fungi, hierro, single-organism biosynthetic process, Migrant Worker, 14C-labeled cpd, formation, anabolism, Triticum aestivum8, Triticum spelta, N, Deferoxamine, Transients, Microbial Community Structure, Nonmigrant, hypoplasia, Triticum aestivam, Humus, Siderophore, Nonmigrants, Cultural Relativisms, plants, Filamentous Fungus, deferoxaminum, Worker, wheat, N'-(5-((4-((5-(acetylhydroxyamino)pentyl)amino)-1, deferrioxamine B, Transient, BcDNA:GH03694, catechol sodium salt, Tricum aestivum, Solution, common wheat, synthesis, Squatter, Environmental Impact, Abilities, reaction, bread wheat, Deferoxamine Mesylate, Drechslera biseptata, Custom, Mesylate, Durum Wheat, Squatters, Microbial Community Structures, Soils, Customs, Triticum turgidum, Helminthosporium biseptatum, deferoxamine, Mesilate, fungi, Deferoxamine Mesilate, Siderochromes, 3-dihydroxybenzene, DmelCG9063, deferoxamina, Human Microbiome, small, amides, Triticum durum, 4-dioxobutyl)hydroxyamino)pentyl)-N-(5-aminopentyl)-N-hydroxy-, bacteria, Deferoxamine Methanesulfonate, Community Composition, Deferrioxamine B, Molds, Deferrioxamine, Triticum vulgare, land plants, Fungi, Aptitudes, Fungus, fer, Triticum turgidum subsp. durum, Mycota, Cultural Backgrounds, Microbial, Mold, Ability, Cultural Belief, Belief, Impact, Environmental, desferrioxamine-B, Cultural Background, uptake, Iron 56, Redox, Cultures, 2-dihydroxybenzene, N'-((3, Migrant, Canadian hard winter wheat, N(4)-{5-[acetyl(hydroxy)amino]pentyl}-N(1)-(5-{4-[(5-aminopentyl)(hydroxy)amino]-4-oxobutanamido}pentyl)-N(1)-hydroxybutanediamide, Nomad, Butanediamide, Workers, 6-dioxo-2, Migrants and Transients, Microbial Communities, Microbiome, Desferrioxamine, ferrum, Microbiomes, Cultural Beliefs, underdeveloped, Iron, DFOB, Microbial Community Compositions, Durum, Microbial Community, BcDNAGH03694, Triticum sativum, catechol, Plant, Community Structure, Talents, fungus, Iron-56, catechol dipotassium salt, 3L6, Fe, Composition, Desferal, 2-benzenediol, Eisen, Durum Wheats, pyrocatechol, higher plants, Deferoximine, Filamentous, rich, Talent, Nomads, Environments, Microbiotas, Migrants, earth, Relativism, iron, Cultural Relativism, Desferroxamine, Deferoxamine B, General activity, dimerunic acid, Oxidation Reduction, Human Microbiomes, Triticum aestivum, Communitysiderochrome catabolism.Triticum durum, ferrum, hierro, Iron, Durum, Triticum vulgare, Triticum aestivum8, Triticum spelta, Triticum sativum, fer, Triticum aestivam, aerial root (narrow), Siderophore, Triticum turgidum subsp. durum, root, Iron-56, Triticum aestivum subsp. aestivum, wheat, GRO:0005338, Tricum aestivum, Fe, common wheat, all, Eisen, bread wheat, Durum Wheats, Iron 56, Drechslera biseptata, climbing root (narrow), Canadian hard winter wheat, Durum Wheat, Triticum turgidum, Helminthosporium biseptatum, Brachysporium biseptatum., 26Fe, iron, Siderochromes, Wheat, Triticum aestivumliquid chromatography tandem mass spectroscopy, LC-MS2, Desferrioxamine, Deferoxamin, desferrioxamine-B, LC/MS/MS, deferoxamina., LCMSMS, Deferrioxamine, determination, DFOB, liquid chromatography-tandem mass spectroscopy, DFO, LC-MSMS, chemical analysis, N(4)-{5-[acetyl(hydroxy)amino]pentyl}-N(1)-(5-{4-[(5-aminopentyl)(hydroxy)amino]-4-oxobutanamido}pentyl)-N(1)-hydroxybutanediamide, LC-MS/MS, liquid chromatography tandem mass spectrometry, deferoxamine, assay, deferoxaminum, deferrioxamine B, LC-MS-MS0falseFrench et al, P. biseptata siderophore degradation, 2023LC-MS/MS analysis of P. biseptata supernatant samples after incubation with DFOB and protochelinMSV00009289838240404