{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive.ucsd.edu/v06/MSV000093570/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"submitter":["Julian Preiner","Stefanie Wienkoop"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=83c312c03b1a4bcb9756c18475857d37"],"submitter_email":["stefanie.wienkoop@univie.ac.at","julian.preiner@univie.ac.at"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["98"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["LTQ Orbitrap Elite"],"species":["Glycine Max (ncbitaxon:3847)"],"submitter_affiliation":["University of Vienna","Univiersity of Vienna"],"description_synonyms":["projections, biochemical pathways, Metabolic Networks, Networks, DUbc9, Ghrfr, Sulphite Oxidase, apparatus, Metabolic Process, ion, HSN1E, Pflanze, Metabolic Concepts, symbiotic process, prevention, viridiplantae, Mendosicutes, nitrate(1-), Readability, devices, Lwr, cromo, responsivity, Rhizobacterium japonicum, 10.5, Concepts, Impacts, encompassing mutualism through parasitism, Environmental Impacts, Metabolism Concept, Phenomenon, XOR, prevention and control, IKKg, 10.9, KEY, Key, integumentum commune, Oxidoreductase, 24Cr, appliances, Eubacteria, F, enzymes, reference sample, Metabacteria, DmelCG2328, Soybean, Elements, catabolism, Evidence Based Toxicologies, N, Soy Beans, beta-particle, Pathways, plants, V, metabolic process resulting in cell growth, Sulphite, Xanthine:NAD+ oxidoreductase, Fast, Bacteria Woese et al. 2024, preventive measures, Environmental Impact, set, reaction, e, Bacteria <bacteria>, e-, Respiratory, Prokaryotae, papilla, Respiratory Nitrate Reductase, biotransformation, Procaryotae, ALDEHYDE OXIDASE, NO3(-), Catabolism, CXXC finger protein 9, 1.17.1.4, Soybeans, deco, Archaebacteria, symbiotic interaction between organisms, Pathway, preventive therapy, Vanadium, AT-AO1, external covering of organism, Process, Metal, Cr, land plants, metabolism resulting in cell growth, Tech, Arabidopsis thaliana aldehyde oxidase 1, lamina, F22D1_130, flanges, NO3, lit, organism surface, Positrons, Metabolic Network, metal elements, Soy Bean, endocrine-CEREBROOSTEODYSPLASIA, T32E8.9, chrome, DmIKKgamma, Ionen, Quinol Nitrate Oxidoreductase, Stickstoff, dIKK, Xanthine Oxidoreductase, Kenny, Fast Electron, shelf, margin of safety, prokaryotes, T32E8_9, VI, secretion, hbl, Evidence-Based Toxicology, l(2)02858, little, Evidence Based, Phytomyxa, l(2)05487, ADCADN, l(2)05486, Epistemology, 20.35, Oxidase, UNQ203/PRO229, Chrom, Phaseolus max, i56, Respiratory Nitrate, shelves, DNA (cytosine-5-)-methyltransferase 1, IKK-gamma, Plant, Electron, Evidence, NITRATE REDUCTASE, projection, ridge, LCK2, i105, beta(-), metal element, cv. Wye, Nitrate Reductase, higher plants, nitrogen, DmelCG16910, integumentary system, NR1, Aldehyde, XD, Deco, Biocatalyst, Micronutrient, eve2, Nitrogenase, XO, 7N, Evidence-Based, dermal system, Prokaryota, Gruppe, CG2328, 14.10, Toxinology, parasitism, conformation, ARABIDOPSIS THALIANA ALDEHYDE OXIDASE 1, Molybdenum-98, Biocatalysts, Dehydrogenase, lamellae, Processes, e(-), Toxicology, instruments, commensalism, CG8598, eubacteria, Network, electron, Metabolic Processes, process of organ, dIKK-gamma, lamella, Aim, surface, AIM, dip4, Vanadium Nitrogenase, Metabolism, DmIKK-gamma, symbiotic interaction between species, Ubc 9, even, dmIKKgamma, Bacteriobiota, symbiosis, nitrate reductase 1, aldehyde oxidase 1, Xanthine oxidoreductase, IKK[[gamma]], Toxicologies, Metabolism Phenomena, Evidence-Based Toxicologies, AOalpha, Based Toxicologies, plantae, DNMT1, reactivity, NITRATE REDUCTASE 1, Dinitrogenase, symbiotic interaction between host and organism, MRK, DNMT1_HUMAN, DmelCG3018, iones, elemental metal, Elektron, soybeans, Reduced ferredoxin:dinitrogen oxidoreductase (ATP-hydrolysing), Metabolic Concept, ridges, AtAO1, Ubc-9, ions, semi, Monera, l(2)01519, soybean, Bacteria (ex Cavalier-Smith 1987), Commensalism, API6, Table, Enzyme, GNR1, IKK, grupos, Xanthine, ECO, Beans, toxic potential, fungi, Transition, Archaebiota, laminae, relational structural quality, Soy, NITRATE ION, Controlled, Electrons, Positron, DNA MTase HsaI, DNA (cytosine-5)-methyltransferase 1, bacteria, Controlling, UBC9, Archaeobacteria, Rhizobacterium, grupo, metals, degradation, Ubc9, Negatron, Based Toxicology, DNMT, Negatrons, enzyme activity, function, MCMT, micronutrients, Hydroponic, group, Impact, Concept, Metabolic Phenomena, Environmental, IKKgamma, Quinol-Nitrate Oxidoreductase, Metabolism Concepts, DmelCG8598, Wolfram, FBgn0010602, equipment, F22D1.130, DNA methyltransferase HsaI, dUbc9, Phenomena, dUBC9, Nitrate, Eve, EVE, ubc9, Fast Electrons, Xanthine oxidase, metabolism, Archebacteria, flange, Metabolic Pathways, organ process, Purine Hydroxylase I, Metabolic Phenomenon, Reductase, ATAO, CXXC9, multicellular organism metabolic process, Xanthine dehydrogenase, negatron, biodegradation, CT-2, ensemble, Metabolic, Dmikkgamma, body surface, symbiotic interaction, Mutualism, prophylaxis, Evidence Based Toxicology, archaea, Bean, proteomic analysis., Rest, trace elements, aldehyde oxidase alpha, l(2)46Ce, azote, Understanding, CG16910, Molybdenum 98, CXXC-type zinc finger protein 9, l(2)46Cg, beta, nitrogeno, l(2)46CFj, host-pathogen interaction, elemental metals, chromium, l(2)46CFh, processes, [NO3](-), Ion, Rhizobium japonicum, l(2)46CFp, control, prokaryote, Dmubc9, Glycine max, Environments, Archaeon, Sulfite, Metabolic Pathway, Quinol-Nitrate, Endosymbiosis, response, Chromium, E(eve), groupe, AAO1, CLEC2C, l(2)46CFg, CG3018, DmUbc9, 1.17.3.2, m.HsaI, Anabolism"],"name_synonyms":["soybean, Soy Bean, reactivity, cv. Wye, Soybeans, soybeans., reaction, higher plants, Metal, Phaseolus max, Soybean, responsivity, Glycine max, land plants, Bean, Soy Beans, Plant, Beans, response, plants, Soy"],"citation_count":["0"],"additional_accession":["PXD047532"]},"is_claimable":false,"name":"Transition metal (W, Mo) stress response strategies of leguminous plants (Glycine max) and their symbiotic partners (B. japonicum)","description":"Tungsten (W) and molybdenum (Mo) are economically important transition elements residing in the chromium group of the periodic table. Due to a myriad of industrial applications, ranging from household appliances to war-ammunition and high-tech products, they are increasingly discharged into the environment. However, little is known about their eco-toxicology. While molybdenum is an important micronutrient and serves as co-factor in various key enzymes of microbes, plants and other higher lifeforms, W-enzymes are only found in some archaea and bacteria. Due to their chemical similarities regarding structure, atomic radii and electron configuration, it is proposed that molybdenum ions bound to co-factors of enzymes can be substituted by tungsten. In plants it has been repeatedly demonstrated that tungsten renders the four molybdoenzymes (nitrate reductase, aldehyde oxidase, xanthine dehydrogenase and sulfite oxidase), functionless if incorporated instead of molybednum. In addition, the rhizobial molybdoenzyme, nitrogenase, was found to retain its function even if tungsten is incorporated into the enzyme.\n\nAlthough it has been shown that both transition metals can be phytotoxic when occurring in excess concentrations, knowledge about their effect on plant metabolic processes is still limited. Due to preliminary experimental data obtained for W, we believe that the two transition metals interfere with integral metabolic pathways, especially phosphorous and nitrogen dependent processes. Additionally, it has already been shown that symbiotically grown leguminous plants respond differently to tungsten toxicity than their non-symbiotically grown counterparts. Still, our understanding of the mechanisms behind the molecular response to tungsten toxicity and similarities to molybdenum metabolism remains limited. We aim to comprehensively understand the molecular mechanisms behind phytotoxicity and stress response induced by excess tungsten and molybdenum as well as possible stress alleviation through Rhizobium symbiosis. \n\nIn order to uncover the mechanisms that govern toxicity of Mo and W in plants as well as to identify possible benefits of bacterial symbiosis, soybean plants inoculated with Bradyrhizobium japonicum and a surface sterilized non-symbiotic control supplied with nitrate (10mM KNO3) were grown in a semi hydroponic setup for three weeks, until nodules were formed and symbiosis was fully established. After these three weeks, three different metal treatments (control, 0.5 mM tungsten and 0.5 mM molybdenum) for were applied for two weeks and subsequently harvested for metabolomic and proteomic analysis.\n","dates":{"publication":"Tue Dec 05 04:33:00 GMT 2023"},"accession":"MSV000093570","cross_references":{}}