{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v07/MSV000094520/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["David MacMillan"],"instrument_platform":["timsTOF Pro 2"],"species":["Homo Sapiens (ncbitaxon:9606)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=d03bd8e2e0304111a217cb10f0360ac7"],"submitter_email":["dmacmill@princeton.edu"],"submitter_affiliation":["Princeton"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["4,931"],"ptm_modification":["UNIMOD:765 - \"Removal of initiator methionine from protein N-terminus.\"","UNIMOD:1384 - \"Methionine oxidation to homocysteic acid.\"","UNIMOD:894 - \"Carboxymethylated DTT modification of cysteine.\"","UNIMOD:1 - \"Acetylation.\""],"data_protocol":[""],"description_synonyms":["projections, MGC130048, other disease, stress granule, nucleocytoplasm, SGCG_HUMAN, ER-Phagy, lamellae, AIP4, A4, Gene, Lipophagy, Synthetases, ATP Dependent Proteolysis Factor 1, protein, biosynthesis, Organelle, Ubiquitin carboxyl extension protein 80, protein-containing complex, process of organ, TYPE, dIKK-gamma, dJ468O1.1, Human, DAGA4, lamella, Membrane Tissues, protein polypeptide chains, Readability, polypeptide chain, diseases, sensitive, integral component of membrane, DmIKK-gamma, 35DAG, Gene Products, disease or disorder, Cellular Autophagy, IL-1RAcPIP, diseases and disorders, HMG-20, dmIKKgamma, protein aggregate, MAM, gamma-SG, IKK[[gamma]], Atrophin-1-interacting protein 4, SCG3, sensitivity, 6720481N21Rik, IKKg, multicellular organismal biosynthetic process, KEY, Key, protoplasm, 1300012C07Rik, study, gamma sarcoglycan, single-organism biosynthetic process, human disease, High Mobility Protein 20, protoplast, pre-mortem, formation, Tissues, anabolism, membrane region, Tissue, Ubiquitin-related 1, proteins, labeling, ridges, C230047C07Rik, Nedd4b, 4930403G24Rik, non-neoplastic, synthesis, allergic reaction, Ligase, APF-1, 40S ribosomal protein S27a, cytoplasmic mRNP granule, IKK, ER Phagy, papilla, Pathologies, ubiquitin, Nucleophagy, region of membrane, gamma-sarcoglycan, disorder, Sensitivity, Homo sapiens disease, laminae, protein tagging activity, intracellular, ADMFD, Membrane Tissue, membrane, 8030492O04Rik, Stress Granule, 35 kDa dystrophin-associated glycoprotein, protein complex, 6.3.2.-, Proteins, lamina, disorders, SG-gamma, integral to membrane, flanges, total expressed protein, medical condition, selective autophagy, cytoplasmic mRNP granule., membranous organ component, Ribophagy, CEP52, Cell, SGCG, LGMD2C, nedd4, IKKgamma, DmIKKgamma, hNEDD4-2, Ubiquitin A-52 residue ribosomal protein fusion product 1, native protein, natural protein, Ubiquitin-related 2, dIKK, Reticulophagy, Protein, shelf, Diseases, Kenny, whole membrane, Autophagocytosis, Synthetase, condition, Cellular, sarcoglycan, A130065M08, internal to cell, ATP-Dependent Proteolysis Factor 1, flange, organ process, Ubiq, Ubiquitin, NEDD4-2, RSP5, covalent modifier, Epistemology, transmembrane, membrane of organ, AIF4, DMDA1, Nedd4-2, Dmikkgamma, Autophagy, shelves, IKK-gamma, Specificity, Itch, Human Ubiquitin, P-body, Understanding, Membrane, gamma (35kDa dystrophin-associated glycoprotein), CG16910, projection, Granule, 4931428G15Rik, ridge, Protein Gene Products, Gene Proteins, processes, disease, living, NEDD4.2, DMDA, DmelCG16910, Specificity and Sensitivity, 60S ribosomal protein L40, 35kD dystrophin-associated glycoprotein, Stress, SCARMD2, NFE2-associated polypeptide 1, Ubiquitin-related, nedd4-2, NAPP1, Proteomes"],"name_synonyms":["living, Cell., pre-mortem, SG disassembly"],"additional_accession":[]},"is_claimable":false,"name":"Molecular Mechanisms of Stress Granule Disassembly Revealed by Chemogenetic Microenvironment Mapping (MicroMap) in Living Cells","description":"Phase-separated condensates are membrane-less intracellular structures comprised of dynamic protein interactions that organize essential biological processes. Understanding the composition and dynamics of these organelles advances our knowledge of cellular behaviors and disease pathologies related to granule dysregulation. In this study, we apply microenvironment mapping (MicroMap) with a novel HaloTag-based platform (HaloMap) to characterize intracellular stress granule dynamics in living cells. After validating the robustness and sensitivity of this approach, we then profile the stress granule proteome throughout the formation and disassembly, and under pharmacological perturbation. These experiments reveal several novel ubiquitin-related modulators, including the HECT-type E3 ligases ITCH and NEDD4L, as well as the ubiquitin receptor TOLLIP, as key mediators of granule disassembly. In addition, we identify an autophagy-related pathway that promotes granule clearance. Collectively, this work establishes a general photoproximity labeling approach for unraveling intracellular protein interactomes and uncovers previously unexplored regulatory mechanisms of stress granule dynamics.","dates":{"publication":"Thu Apr 11 14:45:00 BST 2024"},"accession":"MSV000094520","cross_references":{}}