MassIVEapplication/xmlftp://massive.ucsd.edu/v07/MSV000094574/primaryOK200ProteomicsBenjamin C OrsburnQ ExactiveHomo Sapiens (ncbitaxon:9606)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=518f8428662d45d1992d0b7525b2bd36borsbur1@jhmi.eduJohns HopkinsMassIVE45MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetPilot, Water, Rps17, nicotinamidum, Nikotinsaeureamid, criteria, MeCN, D11Ncvs75, NCMe, Procedures, 3-Pyridinecarboxamide, analogs and derivatives., PILOT, H(2)O, Enduramide, RPS17, Methyl, BOUND WATER, CH3OH, Aging, guidelines, Methylalkohol, oxidane, Vitamin B 3, WATER, agua, pyridine-3-carboxylic acid amide, HOH, M(3)67, method, Techniques, M(3L)i, M(3)S33, M(3)i, Nicobion, Biological, Method, CH3-C#N, method used in an experiment, Studies, B3, Wood Alcohol, Co-Pilot, CG5896, vitamin B3, Nicotinamide, Technique, wood alcohol, Nicotinsäureamid Jenapharm, Senescence, DmelCG2045, anatomical systems, beta-pyridinecarboxamide, fast speed, M, l(3)67BDo, Papulex, min, Minute, Sp2, eau, l(3)dtOA4, 3 Pyridinecarboxamide, Nicotinamid, nicotine acid amide, Solution, Niacinamide, Study, Biological Aging, high speed, DmelCG8137, Reference, column, DmelCG3922, Methodological Studies, U2afbp-rs, wood naphtha, sample, M(3)i[55], Nicotinsaeureamid, dihydridooxygen, SP2, sp2, D9, SP1, Sodium Methoxide, Preparation, dihydrogen oxide, SP3, M(3)RpS17, Reference Standard, EGR-3, G/G, c-SP1, nicotinamida, Standard Preparation, c-SP3, Standard Preparations, nicotinamide, niamide, DmelCG5896, Standardization, Alcohol, 1-(14)C-labeled, nicotine amide, 3H-labeled, Grass, aqua, derivatives, M(3)67C, spn2, Methyl alcohol, U2af1-rs1, Procedure, acetonitrile, H2O, niacin, Irlgs2, M(3)i(55), SP#2, nicotinic acid amide, Aviators, water, 3-carbamoylpyridine, BcDNA:RE44119, Sodium, MeOH, mKIAA0048, Carbinol, Jenapharm, ACETONITRILE, Library, cyanomethane, Hydrogen Oxide, Co-Pilots, carbinol, rpS17, Vitamin B3, Nikotinamid, Vitamin, Standard, Aviator, M(3)q, Rp S17, hydrogen hydroxide, B 3, CG8137, ethanenitrile, Methodological, 3-pyridinecarboxamide, nicotylamide, Methodological Study, anon-EST:Posey48, sample population, acqua, CG3922, plan specification, 4930480I16Rik, Nicotinsäureamid, Wood, Preparations, Spn2, nicotinic amide, spirit of wood, wood spirit, Manuscripts, Standards, M(3L)i[55], Vitamin PP, Co Pilot, Wasser, methyl cyanide, CG2045, [OH2], Methyl Alcohol, m-(aminocarbonyl)pyridine, Methoxidenicotinamida, nicotinamidum, Nikotinsaeureamid, nicotinamide, niamide, determination, 3-Pyridinecarboxamide, nicotine amide, Enduramide, Aging, Spectrum Analyses, Spectrum Analysis, Vitamin B 3, pyridine-3-carboxylic acid amide, Spectroscopy, MS, niacin, Nicobion, Biological, nicotinic acid amide, chemical analysis, 3-carbamoylpyridine, Mass, B3, vitamin B3, Analysis, Nicotinamide, Jenapharm, Mass Spectrum Analyses, Mass Spectroscopy, Nicotinsäureamid Jenapharm, Mass Spectrum Analysis, Senescence, Mass Spectrum, Vitamin B3, Nikotinamid, Vitamin, beta-pyridinecarboxamide, Analyses, Papulex, Spectrometry, B 3, 3-pyridinecarboxamide, 3 Pyridinecarboxamide, Nicotinamid, nicotylamide, nicotine acid amide, Niacinamide, Biological Aging, Nicotinsäureamid, nicotinic amide, Mass., Nicotinsaeureamid, Vitamin PP, assay, m-(aminocarbonyl)pyridinefalseAnalysis of nicotinamide based anti-aging supplements in 2024 by untargeted high resolution mass spectrometry"Anti-aging" supplements purported to contain nicotinamide based compounds were obtained from a variety of sources. Samples were extracted in a solution of 60% methanol at a 1:20 to 1:100 ratio based on pilot tests on solubilization of material. Extracts were clarified by high speed centrifugation, dried by speedvac and diluted to a 1:100 to 1:500 g/g ratio of the original material in 5% acetonitrile in LCMS grade water. Samples were analyzed on a Q Exactive Classic system coupled to a Dionex UHPLC using a 15cm x 2.1mm HyperSil gold column with 1.5um particles (Thermo, all). A 20 minute method with a data dependent top 3 method was used with each sample analyzed in triplicate, separately in each polarity. The output files were analyzed in Compound Discoverer 3.3 SP2 using a combination of libraries as described in the manuscript. Standards were obtained from MilliPore Sigma for Nicotinamide and derivatives for retention time matching and concentation estimations. Sat Apr 20 06:27:00 BST 2024MSV000094574