MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v07/MSV000094686/primaryOK200Benjamin C Orsburnhttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=1140e04b787b441a9192b28dcdbe8afcborsbur1@jhmi.eduMassIVE255UNIMOD:35 - "Oxidation or Hydroxylation."ProteomicstimsTOF fleXAllium Tricoccum (ncbitaxon:138334)Johns HopkinsPhysical Barrier, dtl-b, MUTYH-Associated Polyposis, Access, Zfp198, methionine aminopeptidase activity, MYH-associated polyposis, Physical, autosomal recessive familial adenomatous polyposis, Architectural Barrier, Maps, DCAF2, wild leek, Facility Accesses, Architectural Barriers, Ramp, MYM, ramp, Ramps, 2, RAMP, familial adenomatous polyposis, MAP, peptidase M activity, autosomal recessive, 5730564G15Rik, adenomas, ZNF198, Architectural, RUTBC3, rabGAPLP, Accessibility, L-methionine aminopeptidase activity, Barrier, familial adenomatous polyposis 2, L2DTL, 5730564G15Rik., cdt2-b, RabGAP-5, cdt2-a, Accesses, MYH-Associated Polyposis, l2dtl, RABGAP5, Physical Barriers, autosomal recessive multiple colorectal adenomas, CDT2, 2810047L02Rik, dcaf2, 5830413P05Rik, RUSC3, FIM, multiple colorectal, Znf198, cdt2, Facility Access, MAP syndrome, Barriers, SCLL, Facility, FAP2, colorectal adenomatous polyposismedulla, Water, sodium salt, Rps17, MeCN, NCMe, determination, Pflanze, Physical, H(2)O, RPS17, DCAF2, Ramp, BOUND WATER, Profiles, protein, Stage, GRO:0005338, Life History, oxidane, RP11-508D10.1, viridiplantae, WATER, MYM, Metepencephalon, HOH, Alkylations, M(3)67, Cut, DMU42699, Tnfsf5, Polypeptides, protein polypeptide chains, Techniques, %, M(3L)i, primary metabolites, ramp, exact), peptido, l(1)7Ba, l(1)7Bb, Method, CH3-C#N, Ramps, Life Cycle, Scanning Transmission Electron Microscopy, RAMP, bis(p-chlorophenyl)acetic acid, protein aggregate, primary stem, wood alcohol, 5730564G15Rik, Metabolic Profiles, T-BAM, type 5 acid phosphatase, tartrate-resistant acid ATPase, peptides, entire life cycle, M, l(3)67BDo, min, proteins, CG11387, myelencephalon, CDT2, 2810047L02Rik, dcaf2, TRAP3, T-cell antigen Gp39, Tudor repeat associator with PCTAIRE-2, Methodological Studies, climbing root (narrow), cdt2, Facility Access, CT, dihydridooxygen, D9, TNFSF5, autolysin activity, necrosis, Sodium Methoxide, dihydrogen oxide, T5ap, Dmel_CG6883, MGC - 45012, M(3)RpS17, CD40L, Physical Barrier, anatomical protrusion, Zfp198, Alcohol, Tnfrsf5, Ct, aqua, Cd40l, M(3)67C, wild leek, aerial root (narrow), CT24921, Procedure, hCD40L, CG12369, Electron Microscopy, HDC07747, H2O, DmelCG42865, soluble, M(3)i(55), IMD3, bacteriocin activity, TNF-related activation protein, bHLHe13, BcDNA:GH10590, bulbus, Carbinol, Ly-62, Hydrogen Oxide, carbinol, rpS17, Barrier, life, L2DTL, Life Cycle Stage, Rp S17, NKTL, cdt2-b, Plant, hydrogen hydroxide, History Stage, AW743063, cdt2-a, root, Methodological, Accesses, AI326936, Methodological Study, l2dtl, 葉 (Japanese, acqua, Life Cycles, Wood, lysin activity, precipited material, primary axis, spine, spirit of wood, Dmel_CG13885, metabolites, TrATPase, IGM, STEM, bacteriolytic toxin activity, CD40LG, Wasser, Polypeptide, methyl cyanide, anatomical systems., Methyl Alcohol, dtl-b, lifespan, l(1)VE614, Access, Procedures, TRAP, p50, Ly62, TAPK, secondary metabolites, Gene, stalk, Methyl, CH3OH, vegetative, Methylalkohol, protein-containing complex, TR-AP, agua, protrusion, Buffer, method, M(3)S33, potassium salt, bulb, M(3)i, polypeptide chain, 14C-labeled, method used in an experiment, Cycle, GP39, Gene Products, Studies, Wood Alcohol, Bulb, 3.1, CD40-L, Life, Metabolic Profile, Technique, l(2)k11012, gp39, plantae, CD154, Cycles, anatomical systems, CATC4, GKLP, entire lifespan, Metabolomes, Profile, Minute, metepencephalon, eau, l(3)dtOA4, kf, Life History Stages, culm, Solution, Physical Barriers, Study, PCTAIRE2-binding protein, 5830413P05Rik, MGC:45012, Stages, DmelCG11387, DmelCG3922, TEIF, Life History Stage, wood naphtha, Trap, M(3)i[55], HIGM1, peptidos, Facility, C78062, P105, NTKL, bead, CG42865, 1-(14)C-labeled, 3H-labeled, TRH, Trh, axis, protein complex, PCTAIRE2BP, lac, Proteins, Architectural Barrier, Facility Accesses, Architectural Barriers, Methyl alcohol, Bp50, Scanning Transmission, ACP5, acetonitrile, SPENCDI, buffer, Peptide, all, native protein, natural protein, l(2)k11012b, water, Protein, chemical analysis, BcDNA:RE44119, Sodium, MeOH, DmelCG12369, BP1081, ACETONITRILE, cyanomethane, medulla oblonmgata, DDA, ZNF198, Architectural, Accessibility, History Stages, holin, Metabolic, M(3)q, metabolite, medulla oblonzata, ethanenitrile, CG13885, hoja (Spanish, anon-EST:Posey48, CG3922, Protein Gene Products, plan specification, Gene Proteins, FIM, wood spirit, l(3)10512, Znf198, M(3L)i[55], Peptid, assay, TRACP, Barriers, SCLL, CG6883, [OH2], MethoxidePXD052017falseToward a comprehensive proteomic draft map of the West Virginia Ramp (Allium tricoccum) Samples of the North American wild ramp (wild leak) Allium tricoccum were harvested during the vegetative and dormant stages of the life cycle. The vegatative plant was cut into root, bulb, stem and leaf sections and homogenized in a bead mill in a solution of 35/35/30 LCMS grade acetonitrile, methanol, and water respectively. Centrifugation at 13,000 x g at 5 minutes was used to separate the soluble metabolome and the solid material (protein, etc.,). The soluble solution was dried by speedvac and resuspended in 5 percent acetonitrile in water for metabolomic analysis on a Q Exactive system using a 20 minute DDA method. Metabolites were analyzed in Compound Discverer 3.1. The same process was performed for dormant ramps with the exception that only bulb and root material could be obtained. The precipitate for all samples was solubilized in 1x S-trap lysis buffer and prepared with the S-Trap mini spin column kits following all vendor protocols, with the exception that reduction and alkylation were not performed. The resulting peptides were quantified and 400ng of material was analyzed using a DDA PASEF method on an EvoSep One system. Mon May 06 07:08:00 BST 2024MSV000094686