MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v08/MSV000095231/primaryOK200Jacob B. Gerihttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=0ca00eef3f7b4a7fb7a5234a4c3a226bjag4016@med.cornell.eduMassIVE17,110[C] [137.0840] S-(hex-5-yn-1-ylcarbamoyl)-L-cysteine[C] [438.2845] 3-(6-(4-(4-(2-((2-amino-2-carboxyethyl)thio)acetamido)butyl)-1H-1,2,3-triazol-1-yl)hexyl)-1,1',3'-trimethyl-1H,1'H-[2,2'-biimidazole]-3,3'-diium[C] [422.3118] S-(2-((4-(1-(2-(bis(2-(dimethylamino)ethyl)amino)-2-oxoethyl)-1H-1,2,3-triazol-4-yl)butyl)amino)-2-oxoethyl)cysteine[C] [418.2805] S-(2-oxo-2-((4-(1-(2-oxo-2-(4-(2-(piperazin-1-yl)ethyl)piperazin-1-yl)ethyl)-1H-1,2,3-triazol-4-yl)butyl)amino)ethyl)cysteine[C] [296.1848] S-(2-((2-(6-((4R,5S)-5-methyl-2-oxoimidazolidin-4-yl)hexanamido)ethyl)amino)-2-oxoethyl)cysteine[C] [392.2648] S-(2-((4-(1-(2-(1,4,7,10-tetraazacyclododecan-1-yl)-2-oxoethyl)-1H-1,2,3-triazol-4-yl)butyl)amino)-2-oxoethyl)cysteine[C] [366.2492] S-(2-((4-(1-(2-(bis(2-aminoethyl)amino)-2-oxoethyl)-1H-1,2,3-triazol-4-yl)butyl)amino)-2-oxoethyl)cysteine[C] [432.2962] S-((4-(1-(2-(4-(2-(4-methylpiperazin-1-yl)ethyl)piperazin-1-yl)-2-oxoethyl)-1H-1,2,3-triazol-4-yl)butyl)carbamoyl)cysteine[C] [391.2209] 3-(1-amino-1-carboxy-5-oxo-9,12,15-trioxa-3-thia-6-azaheptadecan-17-yl)-1,1',3'-trimethyl-1H,1'H-[2,2'-biimidazole]-3,3'-diium[C] [297.1683] 3-(1-amino-1-carboxy-5-oxo-9,12,15-trioxa-3-thia-6-azaheptadecan-17-yl)-1-methyl-1H-imidazol-3-ium[C] [530.3805] S-(2-oxo-2-((4-(1-(2-oxo-2-(4-(2-(4-(2-(piperazin-1-yl)ethyl)piperazin-1-yl)ethyl)piperazin-1-yl)ethyl)-1H-1,2,3-triazol-4-yl)butyl)amino)ethyl)cysteineUNIMOD:1 - "Acetylation."[C] [531.36454] S-(2-((4-(1-(2-(4-(2-(4-(2-morpholinoethyl)piperazin-1-yl)ethyl)piperazin-1-yl)-2-oxoethyl)-1H-1,2,3-triazol-4-yl)butyl)amino)-2-oxoethyl)cysteine[C] [518.3220] 3-(2-(1H-imidazol-1-yl)ethyl)-1-(2-(3-(6-(4-(4-(2-((2-amino-2-carboxyethyl)thio)acetamido)butyl)-1H-1,2,3-triazol-1-yl)hexyl)-1H-imidazol-3-ium-1-yl)ethyl)-1H-imidazol-3-iumMOD:01060 - "A protein modification that effectively converts an L-cysteine residue to S-carboxamidomethyl-L-cysteine."[C] [532.3370] 3-(6-(4-(4-(2-((2-amino-2-carboxyethyl)thio)acetamido)butyl)-1H-1,2,3-triazol-1-yl)hexyl)-1-(2-(3-(2-(3-methyl-1H-imidazol-3-ium-1-yl)ethyl)-1H-imidazol-3-ium-1-yl)ethyl)-1H-imidazol-3-ium[C] [283.1532] 17-amino-1-(1H-imidazol-1-yl)-13-oxo-3,6,9-trioxa-15-thia-12-azaoctadecan-18-oic acid[C] [573.4227] S-(2-((4-(1-(2-(4-(2-(bis(2-(piperazin-1-yl)ethyl)amino)ethyl)piperazin-1-yl)-2-oxoethyl)-1H-1,2,3-triazol-4-yl)butyl)amino)-2-oxoethyl)cysteineMOD:00719 - "A protein modification that oxygenates an L-methionine residue to one of the diastereomeric L-methionine sulfoxide residues."[C] [514.3005] 3-(2-(2-(2-(2-(4-(4-(2-((2-amino-2-carboxyethyl)thio)acetamido)butyl)-1H-1,2,3-triazol-1-yl)ethoxy)ethoxy)ethoxy)ethyl)-1,1',3'-trimethyl-1H,1'H-[2,2'-biimidazole]-3,3'-diium[C] [438.2845] 3-(6-(4-(4-(2-((2-amino-2-carboxyethyl)thio)acetamido)butyl)-1H-1,2,3-triazol-1-yl)hexyl)-1-(2-(3-methyl-1H-imidazol-3-ium-1-yl)ethyl)-1H-imidazol-3-iumProteomicstimsTOF Pro 2Homo Sapiens (ncbitaxon:9606)Weill Cornell MedicinefalseSelective Gas Phase Enrichment of Modified Peptides for Activity-Based Protein ProfilingGas phase enrichment separates ionized peptides by their physicochemical properties, offering both simplified sample processing and integration with existing MS technology. We leveraged differential ion mobility of labeled peptides to develop a gas phase enrichment approach for reactive amino acid profiling. A compact cysteine-reactive reagent dramatically increases the ion mobility of labeled peptides and enables their selective fragmentation and sequencing. Wed Jul 03 07:05:00 BST 2024MSV000095231