{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v08/MSV000095249/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"submitter":["Jacob Geri"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=3a0ddee3f45c424fba3d40fa6e1d36eb"],"submitter_email":["jag4016@med.cornell.edu"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["1,884"],"ptm_modification":["UNIMOD:4 - \"Iodoacetamide derivative.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["timsTOF Pro 2"],"species":["Plasmodium Falciparum (ncbitaxon:5833)"],"submitter_affiliation":["Weill Cornell Medicine"],"description_synonyms":["small, sodium salt, big, molecula., bead, Procedures, gyltl1b-b, Proteins, Plasmodium, Mbp1, Gene, neutral molecular compounds, Procedure, Strepavidin, Cell, Peptide, froggy, Gyltl1a, Polypeptides, method, Techniques, large, potassium salt, peptido, reduced, Method, MDDGA6, 14C-labeled, method used in an experiment, mKIAA0609, Protein, Studies, Gene Products, tiny, bis(p-chlorophenyl)acetic acid, myd, KIAA0609, molecule, Technique, molecula, DDA, fg, molecules, gyltl1b, peptides, underdeveloped, Plasmodium (Laverania) falciparum, MDDGB6, ligand, mdc1d, hypoplasia, expanded, Mbp-1, labeling, LARGE, Molekuel, Methodological, Methodological Study, free, plan specification, Protein Gene Products, Study, Biotinylations, Gene Proteins, BPFD#36, MDC1D, Methodological Studies, enr, enlarged, Acetamide, great, falciparums, Peptid, peptidos, Polypeptide, malaria parasite P. falciparum, Plasmodium falciparums, 2-iodo-"],"name_synonyms":["Macronutrients, screening, findings, Screenings, survival, Parasite, death rate, Mass Screenings, time of survival., nutrients, symptoms, Mass, Screening, signs, Nutrient, Macronutrient"],"additional_accession":["PXD053644"]},"is_claimable":false,"name":"Phenotypic screening against P. falciparum under physiologically relevant nutrients levels reveals novel chemical diversity and highlights the importance of the PSAC channel for parasite survival.","description":"Microenvironment mapping was used to identify the binding targets of two small molecules in Plasmodium Falciparum cells. In the method, small molecule - photocatalyst conjugates sensitize biotinylation of proteins binding the small molecules, which were reduced, alkylated with iodoacetamide, and immunoprecipitated using streptavidin beads. Labeling was subject to offcompete using a large excess of the free small molecule. On-bead tryptic digestion yielded peptides, which were subjected to DDA-PASEF MS measurement using a timsTOF Pro 2. CLAG3 was significantly enriched by labeling by both molecules. ","dates":{"publication":"Thu Jul 04 09:34:00 BST 2024"},"accession":"MSV000095249","cross_references":{}}