MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v08/MSV000095249/primaryOK200Jacob Gerihttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=3a0ddee3f45c424fba3d40fa6e1d36ebjag4016@med.cornell.eduMassIVE1,884UNIMOD:4 - "Iodoacetamide derivative."ProteomicstimsTOF Pro 2Plasmodium Falciparum (ncbitaxon:5833)Weill Cornell Medicinesmall, sodium salt, big, molecula., bead, Procedures, gyltl1b-b, Proteins, Plasmodium, Mbp1, Gene, neutral molecular compounds, Procedure, Strepavidin, Cell, Peptide, froggy, Gyltl1a, Polypeptides, method, Techniques, large, potassium salt, peptido, reduced, Method, MDDGA6, 14C-labeled, method used in an experiment, mKIAA0609, Protein, Studies, Gene Products, tiny, bis(p-chlorophenyl)acetic acid, myd, KIAA0609, molecule, Technique, molecula, DDA, fg, molecules, gyltl1b, peptides, underdeveloped, Plasmodium (Laverania) falciparum, MDDGB6, ligand, mdc1d, hypoplasia, expanded, Mbp-1, labeling, LARGE, Molekuel, Methodological, Methodological Study, free, plan specification, Protein Gene Products, Study, Biotinylations, Gene Proteins, BPFD#36, MDC1D, Methodological Studies, enr, enlarged, Acetamide, great, falciparums, Peptid, peptidos, Polypeptide, malaria parasite P. falciparum, Plasmodium falciparums, 2-iodo-Macronutrients, screening, findings, Screenings, survival, Parasite, death rate, Mass Screenings, time of survival., nutrients, symptoms, Mass, Screening, signs, Nutrient, MacronutrientPXD053644falsePhenotypic screening against P. falciparum under physiologically relevant nutrients levels reveals novel chemical diversity and highlights the importance of the PSAC channel for parasite survival.Microenvironment mapping was used to identify the binding targets of two small molecules in Plasmodium Falciparum cells. In the method, small molecule - photocatalyst conjugates sensitize biotinylation of proteins binding the small molecules, which were reduced, alkylated with iodoacetamide, and immunoprecipitated using streptavidin beads. Labeling was subject to offcompete using a large excess of the free small molecule. On-bead tryptic digestion yielded peptides, which were subjected to DDA-PASEF MS measurement using a timsTOF Pro 2. CLAG3 was significantly enriched by labeling by both molecules. Thu Jul 04 09:34:00 BST 2024MSV000095249