MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v08/MSV000095342/primary200OKJohn R. Yates IIIhttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=2fd701f792b843079bf0bf133742a406jyates@scripps.eduMassIVE71MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetProteomicsOrbitrap EclipseOrbitrap Fusion LumosMus Musculus (ncbitaxon:10090)The Scripps Research Institute4-d]imidazol-4-yl)pentanoic acid, Deacura, 2-amino-4-(methylsulfanyl)butanoic acid, vitamin B7, Biotine, Aminosaeure, Aminocarbonsaeure, NI220|250, Medebiotin, L Isomer, Amino acid, Gene, My043, protein, alpha-amino acid, Vitamin H, Spectrum Analyses, SMADIP, protein-containing complex, Polypeptides, L-Isomer Methionine, protein polypeptide chains, Foocen, %, MADHIP, Methionine, SP1507, peptido, polypeptide chain, Biotin, NSP, Nsp, 4beta, Gene Products, PPP1R173, KIAA0886, Mass, L-Methionine, Neuroendocrine-specific protein C homolog, 6aR)-Hexahydro-2-oxo-1H-thieno[3, Nbla10545, Analysis, protein aggregate, 6aalpha))-, Gelfert, Pedameth, Mass Spectroscopy, Mass Spectrum Analysis, Biotin Hermes, amino acids, NOGO-A, peptides, Coenzyme R, Analyses, M, NOGO, 4930441F12Rik, (3aS, cis-Hexahydro-2-oxo-1H-thieno(3, biotina, labeling, biotine, proteins, 4-d]imidazole-4-valeric acid, reaction, Hermes, 2-amino-4-(methylthio)butanoic acid, ASY, (+)-cis-Hexahydro-2-oxo-1H-thieno[3, Neurite outgrowth inhibitor, E030027L17, 0710005K15Rik, peptidos, biotinum, Rtn1-b, metionina, Rtn1-c, Racemethionine, Neuroendocrine-specific protein, Rtn1-a, thiol methyltransferase activity, Alkyne, Medobiotin, hSARA, NI220/250, Rombellin, NOGOC, DL-Methionine, Nbla00271, protein complex, Aminokarbonsaeure, Madh-interacting protein, Proteins, HCMOGT1, 2-Amino-4-(methylthio)butyric acid, total expressed protein, Roche, Biotin Gelfert, alpha-amino acids, Reticulon-5, Nogo-B, Nogo-C, L-Isomer, 5-(2-oxohexahydro-1H-thieno[3, NSP-CL, Spectrum Analysis, alpha-amino carboxylic acids, 4]imidazole-4-valeric acid, RTN-x, Peptide, 4S, 4-d)imidazole-4-pentanoic acid, (3aS-(3aalpha, Spectroscopy, TPSG1, S-adenosyl-L-methionine:thiol S-methyltransferase activity, cis-Tetrahydro-2-oxothieno(3, MS, 4]imidazoline-4-valeric acid, biotin, native protein, natural protein, RTN4-A, Novel serine protease, Protein, RTN4-C, Biotin Ratiopharm, Methionin, Madhip, CYTSB, Hmet, 4-d)imidazoline-4-valeric acid, Acetylenes, Mass Spectrum Analyses, Acetylenic Compounds, Met, RTN-X, D-Biotin, Biodermatin, Receptor activation anchor, Amino Acid, Acid, Biotin-Ratiopharm, 1H-Thieno(3, Mass Spectrum, Smad anchor for receptor activation, Biotine Roche, Gabunat, Amino acids, Protein., RTN4-B2, RTN4-B1, Nogo protein, hexahydro-2-oxo-, Spectrometry, HCMOGT-1, Amino, 4)imidazole-4-valeric acid, SARA, Protein Gene Products, Gene Proteins, R75279, PRSS31, alpha-amino-gamma-methylmercaptobutyric acid, cis-(+)-Tetrahydro-2-oxothieno[3, Peptid, Acids, Polypeptide, D-(+)-biotin, Liquimeth, TMT, Biokur, Proteomes, Mothers against decapentaplegic homolog-interacting proteinMedobiotin, 4-d]imidazol-4-yl)pentanoic acid, Deacura, vitamin B7, Rombellin, determination, Biotine, acid, Medebiotin, Roche, Biotin Gelfert, Vitamin H, 5-(2-oxohexahydro-1H-thieno[3, 4]imidazole-4-valeric acid, Peptide, 4S, 4-d)imidazole-4-pentanoic acid, (3aS-(3aalpha, Polypeptides, cis-Tetrahydro-2-oxothieno(3, 4]imidazoline-4-valeric acid, biotin, peptido, Biotin, sensitive, 4beta, Biotin Ratiopharm, 6aR)-Hexahydro-2-oxo-1H-thieno[3, 4-d)imidazoline-4-valeric acid, 6aalpha))-, Acetylenes, Gelfert, sensitivity, Acetylenic Compounds, D-Biotin, Biodermatin, Acid, Biotin-Ratiopharm, 1H-Thieno(3, Biotin Hermes, Biotine Roche, Gabunat, peptides, Coenzyme R, Saeuren, acide, (3aS, cis-Hexahydro-2-oxo-1H-thieno(3, biotina, hexahydro-2-oxo-, Specificity, acids, chemical analysis., biotine, acido, 4)imidazole-4-valeric acid, allergic reaction, 4-d]imidazole-4-valeric acid, Hermes, Specificity and Sensitivity, (+)-cis-Hexahydro-2-oxo-1H-thieno[3, cis-(+)-Tetrahydro-2-oxothieno[3, Sensitivity, Peptid, peptidos, Polypeptide, assay, biotinum, Saeure, D-(+)-biotin, Biokur, AlkynePXD053959falseAcid cleavable biotin-alkyne improves sensitivity for direct detection of biotin labeled peptides in BONCAT analysis.BONCAT (Biorthogonal noncanonical amino acid tagging) is a labeling strategy that covalently adds a biotin-alkyne (BA) to methionine analogs via a click reaction. When methionine analogs are incorporated into a proteome, enrichment of the BA-labeled proteins allows the detection of newly synthesized proteins (NSP) by mass spectrometry. We previously reported that using our Direct Detection of Biotin-containing Tags (DidBIT) strategy, protein identifications and confidence are increased by enriching for BA-peptides instead of BA-proteins. We compared cleavable BA (DADPS) and uncleavable BA in the identification and TMT quantification of NSP. More than fifty percent more proteins were identified and quantified using DADPS than with uncleavable BA. Interrogation of the data revealed that multiple factors are responsible for the superior performance of DADPS.Mon Jul 15 16:11:00 BST 2024MSV000095342