{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v09/MSV000097213/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"submitter":["Edward Marcotte"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=6d7ceb8b993c4d6181bb312bb6350cbd"],"submitter_email":["marcotte@icmb.utexas.edu"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["25"],"ptm_modification":["UNIMOD:765 - \"Removal of initiator methionine from protein N-terminus.\"","UNIMOD:766 - \"Removal of initiator methionine from protein N-terminus, then acetylation of the new N-terminus.\"","UNIMOD:1 - \"Acetylation.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["Orbitrap Fusion Lumos"],"species":["Dictyostelium Discoideum Ax2 (ncbitaxon:366501)"],"submitter_affiliation":["University of Texas-Austin"],"description_synonyms":["d230, Size, determination, Gel Permeation, Molecular Sieve, Gene, dTAFII250, Gel Filtration Chromatography, protein, Spectrum Analyses, Ion Exchange, protein-containing complex, EfW1, dmTAF[[II]]230, protein polypeptide chains, polypeptide chain, dmTAF1, Gel Filtration, Taf230, Gel, Gene Products, Mass, Analysis, protein aggregate, Mass Spectroscopy, TAF250, Mass Spectrum Analysis, Taf200, dTAF[[II]]250, TFIID TAF250, Analyses, cel, cell, Molecular, Dictyostelium discoideum, Dictyosteliums, Ion-Exchange, Taf1p, proteins, Exclusion, Ion-Exchange Chromatographies, dTAF250, Dictyostelium discoideums, Size Exclusion Chromatography, Chromatography, site, Dictyostelium, Molecular Sieve Chromatography, TAF, Chromatographies, Sieve Chromatography, Gene., dTAF[[II]]230, TAF[[II]]250, Filtration Chromatography, protein complex, Proteins, TAF200, l(3)84Ab, BG:DS00004.13, TAFII-250, TAF250/230, Spectrum Analysis, Cell, dTAF230, Spectroscopy, Filtration, Ion Exchange Chromatography, TAFII250, MS, native protein, natural protein, Gel Permeation Chromatography, p230, Gel Chromatography, chemical analysis, Protein, TAF[[II]]250/230, sequence, TFIID, Exclusion Chromatography, region, Mass Spectrum Analyses, Taf[[II]]250, Mass Spectrum, TAF[[II]]230, Spectrometry, TAF[II]250, discoideum, CG17603, TAF[[II]], primary structure of sequence macromolecule, Size Exclusion, Protein Gene Products, Gene Proteins, Ion-Exchange Chromatography, DmelCG17603, Taf250, SR3-5, assay, Ion Exchange Chromatographies, TAF230, TAF1"],"name_synonyms":["Repeated Rounds of Survey, Questionnaire, protein complex, Design, Proteins, Gene, Dictyostelium., protein, Survey Methods, Questionnaire Design, protein-containing complex, Questionnaire Designs, Techniques, protein polypeptide chains, native protein, natural protein, polypeptide chain, Baseline, Survey Methodology, Protein, Community Survey, Baseline Surveys, Gene Products, Survey, Randomized, Nonrespondents, Randomized Response Techniques, protein aggregate, Randomized Response, Methods, Community Surveys, Response Techniques, Methodology, Response Technique, Nonrespondent, Dictyostelium discoideum, Designs, Dictyosteliums, proteins, Questionnaires, Respondent, Survey Method, Questionnaires and Surveys, discoideum, Protein Gene Products, Gene Proteins, Baseline Survey, Dictyostelium discoideums, Respondents, Surveys, Randomized Response Technique, Community"],"additional_accession":["PXD061189"]},"is_claimable":false,"name":"Survey of megadalton protein assemblies from Dictyostelium discoideum","description":"The analysis of high-abundance megadalton protein complexes in Dictyostelium discoideum cell lysates was conducted as follows: the solubilized protein fraction was subjected to size-exclusion chromatography isolating fractions corresponding to the megadalton region, followed by ion-exchange chromatography to further resolve the protein complexes. The fractions obtained after both chromatographic steps were then analyzed using mass spectrometry to identify the constituent protein complexes, which provided the necessary data for cryo-EM.","dates":{"publication":"Tue Feb 25 11:43:00 GMT 2025"},"accession":"MSV000097213","cross_references":{}}