{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v09/MSV000097696/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Sahar El Aidy","Gilles van Wezel"],"instrument_platform":["Q Exactive HF"],"species":["Pseudomonas Fluorescens (ncbitaxon:294)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=6bfdcb062ce0497585c222ccffa0bbe9"],"submitter_affiliation":["Institute of Biology, Leiden University","Swammerdam Institute for Life Sciences (SILS), University of Amsterdam"],"submitter_email":["g.wezel@biology.leidenuniv.nl","s.elaidy@uva.nl"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["56"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"pubmed_abstract":["<h4>Background</h4>Pseudomonas fluorescens is a Gram-negative bacterium with a remarkable metabolic and physiological versatility that enables it to adapt and colonize diverse ecological niches, including the human small intestine. While serotonin is primarily found in high concentrations in gut tissue, its levels in the lumen can be elevated in conditions such as celiac disease, where P. fluorescens is also found in increased abundance. The potential effects of serotonin on P. fluorescens in such contexts remain unclear.<h4>Results</h4>We demonstrate that P. fluorescens metabolizes serotonin primarily into 5-hydroxyindole-3-acetic acid (5-HIAA) and, to a lesser extent, into 5-hydroxytryptophol and N-acetylserotonin. Gene expression analysis revealed significant changes in oxidative stress-related pathways over time, and proteomic analysis confirmed the shifts seen particularly in amino acid catabolic pathways. Serotonin metabolism also enhanced bacterial resistance to oxidative stress, suggesting a protective role.<h4>Conclusions</h4>The findings reveal a novel mechanism by which serotonin modulates the metabolism and stress responses of P. fluorescens. This study provides insight into how P. fluorescens adapts to serotonin-rich environments, such as in celiac disease, and may inform future research on microbial interactions with host-derived metabolites in disease contexts."],"pubmed_title":["Serotonin modulation of metabolism and stress response in Pseudomonas fluorescens."],"pubmed_authors":["Waclawiková Barbora B, Schwalbe Markus M, Ilyaskina Diana D, Toptas Semih S, Thome Nicola U NU, Du Chao C, Elsayed Somayah S SS, de Jong Anne A, van Wezel Gilles P GP, El Aidy Sahar S"],"additional_accession":[]},"is_claimable":false,"name":"Pseudomonas fluorescens - response to serotonin (proteomics)","description":"Pseudomonas fluorescens MFY63 (isolated from human) was incubated with and without 100 micromolar serotonin. The cell pellets were collected after 12 or 24 hours of incubation and the proteome was extracted.","dates":{"publication":"Wed Apr 23 03:59:00 BST 2025"},"accession":"MSV000097696","cross_references":{"pubmed":["41174663"]}}