{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v09/MSV000097888/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"submitter":["Abhay Moghekar","Roland Bruderer"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=00eb30f68bb44f008abdc4f9de6be1dd"],"submitter_email":["roland.bruderer@biognosys.com","m@jhmi.edu"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["231"],"ptm_modification":["UNIMOD:893 - \"Carbamidomethylated DTT modification of cysteine.\"","UNIMOD:35 - \"Oxidation or Hydroxylation.\""],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["Orbitrap Exploris 480"],"species":["Homo"],"submitter_affiliation":["Johns Hopkins University","Biognosys AG"],"name_synonyms":["Plasma, Fresh, average, Cerebro Spinal Fluid, Fresh Frozen Plasmas, Fresh Frozen, Blood Plasma, Frozen Plasma, peptides, Fresh Frozen Plasma, Blood, Fluids, Cerebrospinal Fluids, portion of plasma, spinal fluid, CSF, Aging, Cognitive., Cerebro, Plasmas, Peptide, Spinal Fluid, Cerebrospinal, Cerebro Spinal Fluids, polypeptide, peptide, Frozen Plasmas, Polypeptides, Spinal Fluids, Fluid, cerebral spinal fluid, peptido, Blood Plasmas, Cerebro Spinal, liquor cerebrospinalis, Peptid, peptidos, Polypeptide, plasma"],"description_synonyms":["Amyloid intracellular domain 57, APO-E, Amyloid intracellular domain 59, Materials, Surrogate Endpoints, Laboratory, A4, Amyloid intracellular domain 50, Downregulation, phosphorylation, 5730420M11Rik, AAA, Cvap, Polypeptides, protein polypeptide chains, CG42318, AG, cerebral spinal fluid, insulin-like growth factor receptor ligand, Biological, symptoms, Vkg, Analysis, Extracellular Matrices, IGF receptor binding, AID(50), adult, average, treatment, Senescence, Fresh Frozen, SET, Peripheral Insulin Like Peptides, Ag, Analyses, Hs.22998, Isoproteins, proteins, axon growth, SCZD17, DmelCG4299, Alzheimer disease amyloid A4 protein homolog, N-APP, Biological Aging, set, Gamma-secretase C-terminal fragment 50, Cognitions, GPIa*, Gamma-secretase C-terminal fragment 57, Functions, Gamma-secretase C-terminal fragment 59, xigf1, AD1, AD2, Apo-E, Insulin Like Peptides, Insulin Like, Insulin Like Growth Factor, plasma, screening, Somatomedin C, Viral, S-APP-alpha, CT25584, Up-Regulation (Physiology), CG17144, POREN2, IBP1, Insight, insulin-like growth factor, igf1-A, Spectrum Analysis, signal transduction by trans-phosphorylation, AI255918, Marker, IGF-I-SmC, alpha(IV)2/vkg, Cerebral vascular amyloid peptide, LAMB2T, HLA-DR-associated protein II, End Points, DI-2, Upregulation, I-2Dm, Spectrometry, signs, INSDC_feature:gene, Immunologic, Laboratory Marker, Cerebro, AGE, I-2PP1, TAF-IBETA, signaling pathway, Material, TAF-Ibeta, Fresh, Fresh Frozen Plasma, DmelCG42318, conformation, Clinical Marker, Insulin-Like Peptides, GM-CSF, protein-containing complex, betaApp, AID(59), Growth Factors, APOEA, Insulin, Gene Products, Cerebro Spinal, Growth Factor, C030017F07Rik, lipid metabolism, Clinical, PreA4, Receptor Up-Regulation, Peripheral Insulin-Like, Peripheral ILPs, 2pp2a, S-APP-beta, Dmel_CG17144, CG5620, Gamma-CTF(59), CG10574, Cognitive Functions, AID(57), DmColA2, 2PP2A, Like Peptides, Extracellular, dSET, dSet, coll. IV, peptidos, Biologic, Insulin-Like Growth Factors, Amyloidogenic glycoprotein, findings, Central ILPs, Proteins, Serum Markers, Fluids, Phosphorylations, spinal fluid, Factor, Spinal Fluid, Down Regulation, EMILIN4, Immune Marker, polypeptide, Frozen Plasmas, Spinal Fluids, N-acetyl-D-glucosamine 2-epimerase, MS, Down-Regulation, Fluid, native protein, I-2PP2A, Surrogate End Point, Down-Regulation (Physiology), chemical analysis, Dm I-2, signalling cascade, Mass Spectrum Analyses, A230068P09Rik, Biologic Markers, Insulin-Like Growth, Col4a-2, ensemble, Surrogate, Endpoints, Function, CSF, C31, Gene Proteins, Apo E, LAMNB2, Central Insulin-Like, Receptor, PN2, Beta-APP42, Beta-APP40, General activity, ABPP, Immune Markers, APP, Apo E Isoproteins, ABETA, Cognitive Function, IPP2A2, Coll IValpha2, Biological Markers, Viral Marker, Activity, determination, Blood, cleavage, Biochemical, CTFgamma, Endpoint, protein, Aging, Serum, Abpp, peptide, Insulin Like Growth Factor I, Insulin-Like Somatomedin Peptide I, B2T, Laboratory Markers, peptido, Beta-amyloid protein 42, Beta-amyloid protein 40, l(2)01209, liquor cerebrospinalis, Peripheral, protein aggregate, Mass Spectrum Analysis, signal transduction by protein phosphorylation, Fresh Frozen Plasmas, Insulin-like growth factor I, 1700062G21Rik, mKIAA0578, peptides, TAF-I, apo-E, Central Insulin Like Peptides, ApoE, APOE, IGAAD, Immune, Markers, DmelCG10574, Viral Markers, ICH, disease management, Therapies, C730016P09Rik, Abeta, Apolipoprotein E Isoproteins, APPI, VkgC, C80, mKIAA0343, C83, phapii, Therapy, Cerebro Spinal Fluid, IGF-I, Frozen Plasma, CG16858, AICD-50, Surrogate Endpoint, Igf-I, signal transduction by conformational transition, StF-IT-1, CR32097, Biochemical Markers, Insulin Like Somatomedin Peptide I, col4a2, Gamma-CTF(57), Plasmas, Biologic Marker, Cerebro Spinal Fluids, Spectroscopy, LDLCQ5, C99, ILPs, signaling cascade, Receptor Down-Regulation, IGF-1, RENBP, Lipid, AICD-57, Genetic Materials, Igf-1, Adults, Genetic Material, AICD-59, clotting, Factors, CG4299, Alzheimer disease amyloid protein, Colony-stimulating factor, Csfgm, Protease nexin-II, Dmel_CG5620, Treatments, Biochemical Marker, PTHSL2, Cistron, Insulin-Like Growth Factor, Polypeptide, i2pp2a, nuclear mRNA cis splicing, Central Insulin-Like Peptides, Clinical Markers, 6072, P3(40), Matrix, Gene, Spectrum Analyses, Cognitive., Matrices, PHAPII, 9330127H16Rik, Cerebrospinal, Surrogate End Points, Surrogate Markers, Insulin-Like, polypeptide chain, Metabolism, APP-C57, Insulin-Like Peptides (ILPs), APP-C59, Mass, GMCSF, Peripheral Insulin-Like Peptides, Mass Spectroscopy, study, LPG, Biomarker, Blood Plasma, matrisome, Insulin Like Growth Factors, Genetic, BM600, Up Regulation, Biological Marker, ipp2a2, C130076O07Rik, igf-1, EBR2, signalling pathway, via U2-type spliceosome, Molgramostin, Immunologic Markers, taf-ibeta, Blood Plasmas, Cerebral Spinal Fluid, ECM, signal transduction by cis-phosphorylation, relational structural quality, Immunologic Marker, Apoproteins E, Peptides, Central, protein complex, E030013M08Rik, RnBP, EBR2A, igaad, GlcNAc 2-epimerase, End Point, Cerebrospinal Fluids, DmelCG16858, Cistrons, Peptide, group, Gamma-CTF(50), APP-C99, Insights, natural protein, Sargramostim, Adap, Protein, I2PP2A, Bravo, Plasma, Mass Spectrum, P3(42), PN-II, Serum Marker, NRXN1, Apoprotein (E), portion of plasma, Apolipoprotein E, Soluble APP-beta, Cognitive, Surrogate Marker, Protein Gene Products, dSET/TAF-Ibeta, 2610030F17Rik, Therapeutic, Soluble APP-alpha, renin-binding protein, Peptid, Treatment, assay, MMRN, AA407739, CVAP, 1209"],"additional_accession":["PXD063924"]},"is_claimable":false,"name":"Unveiling Proteomic and Peptide-Level Modifications in Cerebrospinal Fluid and Plasma in Normal Cognitive Aging","description":"This study presents a comprehensive proteomic and peptide level analysis of matched cerebrospinal fluid (CSF) and plasma samples from cognitively normal young (n=52) and older (n=40) adults, employing mass spectrometry to elucidate protein-level and peptide-level changes associated with normal cognitive aging. Differential abundance analysis revealed significant upregulation of extracellular matrix components (ECM), coagulation, and inflammatory pathways in CSF and downregulation of insulin-like growth factor-1 (IGF-1) signaling pathway in plasma with aging based on gene set enrichment and weighted correlation network analyses. Novel alternative protein cleavage/splicing and phosphorylation patterns were identified in many proteins involved in lipid metabolism, ECM structure, axonogenesis and synaptic activity such as APP, APOE, COL4A2, NRXN1, and NRCAM. The examination of alternative cleavage/splicing and phosphorylation events in CSF and plasma provided unprecedented insights into age-associated protein modifications. These findings offer potential biomarkers and therapeutic targets and highlight the complex molecular landscape of normal cognitive aging.\n","dates":{"publication":"Wed May 14 04:58:00 BST 2025"},"accession":"MSV000097888","cross_references":{"TAXONOMY":["9605"]}}