MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v09/MSV000097955/primaryOK200Midhat S Farooqihttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=9d2254e0b3234576a23d3eeacc459a99msfarooqi@cmh.eduMassIVE52UNIMOD:4 - "Iodoacetamide derivative."UNIMOD:35 - "Oxidation or Hydroxylation."ProteomicsOrbitrap AscendHomo Sapiens (ncbitaxon:9606)Children's Mercy HospitalPXD064162falseIntegrated multi-omic analysis reveals novel subtype-specific regulatory interactions in pediatric B-cell acute lymphoblastic leukemiaIn this study, we performed proteomic and phosphoproteomic profiling of B-cell acute lymphoblastic leukemia (B-ALL) samples collected at diagnosis and remission from pediatric leukemia patients with one of two subtypes of B-ALL: BCR::ABL1-like (Ph-like) and ETV6::RUNX1. We analyzed each dataset, as well as existing RNAseq data at diagnosis, to identify subtype-specific features, including increased calcium-dependent signaling in Ph-like samples. We then performed an integrated analysis of all three datasets, which enabled us to identify multiple layers of regulation, including subtype-specific phosphorylation of known cancer-associated proteins. Taken together, these data add to our understanding of the molecular profile of Ph-like and ETV6::RUNX1 B-ALL, demonstrating the utility of multi-omic comparison in pediatric leukemia subtype characterization. Wed May 21 11:48:00 BST 2025MSV000097955