{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v10/MSV000098399/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Dr. Nathan Basisty"],"instrument_platform":["Q Exactive HF"],"species":["Mus Musculus (ncbitaxon:10090)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=45e490f16bad4edfae528dd52422c2aa"],"submitter_email":["nathan.basisty@nih.gov"],"submitter_affiliation":["NIH-National Institute on Aging, Baltimore, MD"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["259"],"ptm_modification":["UNIMOD:4 - \"Iodoacetamide derivative.\""],"data_protocol":[""],"name_synonyms":["Mus musculus, Life Span, lifespan, Lifespans, Life Spans, Laboratory, Swiss, entire lifespan, mice, entire life cycle, Blood, Length of Life, Mus domesticus, number, mouse, Swiss Mouse, life., House Mice, Swiss Mice, Blood Serum, Serum, presence, House Mouse, domesticus, Laboratory Mice, count in organism, House, Mus, Mus musculus domesticus, Mouse, house mouse, Mice, Laboratory Mouse, Serums, Lifespan"],"description_synonyms":["Cognitive Function, Biological Markers, Viral Marker, Materials, Surrogate Endpoints, determination, Laboratory, Blood, Glukose, Mus domesticus, Liquid Chromatography Mass Spectrometry, Biochemical, High Performance Liquid Chromatography Mass Spectrometry, Monohydrate, Endpoint, Ultra Performance Liquid Chromatography-Mass Spectrometry, protein, Aging, (DL)-Isomer, Serum, House Mouse, Nanocrystal, High Performance Liquid Chromatography-Mass Spectrometry, DmelCG11081, protein polypeptide chains, Laboratory Markers, Hydroxyethylpalmitamide, Palmitoylethanolamide, HPLC-MS, Liquid Chromatography-Mass, Biological, DPlexA, Dextrose, Palmitamide MEA, Pisum sativum L., Work Flow, protein aggregate, Senescence, Ultra Performance Liquid Chromatography Mass Spectrometry, me75, Swiss Mice, proteins, D17Mit170, CG17245, T1, DL-glucose, glucose, Biological Aging, Body Compositions, Cognitions, Immune, Markers, Functions, Liquid Chromatography-Mass Spectrometries, Viral Markers, house mouse, garden pea, Viral, Surrogate Endpoint, Nanocrystalline Materials, mouse, Biochemical Markers, Blood Serum, Tl3, Tl2, Biologic Marker, Workflows, Marker, RENBP, (alpha-D)-Isomer, PlexA1, Palmitic acid monoethanolamide, BcDNA:GM05237, Mus musculus, D-Glucose, End Points, PlexB, PlexA, High Pressure Liquid Chromatography Mass Spectrometry, mice, Swiss Mouse, Spectrometry, peas, hexadecanoyl ethanolamide, Immunologic, Laboratory Marker, Plex1, domesticus, AGE, Composition, Material, D Glucose, Palmitinsaeure-beta-hydroxyethylamid, Biochemical Marker, Pea, PEA, Liquid, Mouse, N-palmitoylethanolamine, Glucose Monohydrate, PLEXB, Palmitoyl-EA, Proteomes, Work Flows, gluco-hexose, Pisum, Glucose, Clinical Markers, CG8241, pea, Clinical Marker, Nanocrystalline, number, Gene, CG11081, protein-containing complex, presence, Surrogate End Points, Peas, Surrogate Markers, polypeptide chain, House, Gene Products, Mus musculus domesticus, prp22, D-Plex A, Low, Mice, N-hexadecanoylethanolamine, UPLC-MS, Biomarker, Ultra-Performance Liquid Chromatography-Mass Spectrometry, Clinical, Swiss, Biological Marker, plex A, plex B, Spectrometries, DmelCG17245, Cognitive Functions, palmitoylethanolamide, Immunologic Markers, Nanocrystals, biological marker, Chromatography-Mass Spectrometries, Immunologic Marker, Anhydrous, Biologic, Nanocrystalline Material, Compositions, Nanoparticle, cou, (beta-D)-Isomer, protein complex, RnBP, Monoethanolamine palmitic acid amide, Proteins, Serum Markers, total expressed protein, GlcNAc 2-epimerase, End Point, High Pressure Liquid Chromatography-Mass Spectrometry, plex, DmelCG8241, Immune Marker, Insights, count in organism, N-acetyl-D-glucosamine 2-epimerase, Lr, Cytokine, native protein, natural protein, Mus, Surrogate End Point, Protein, chemical analysis, Anandamide (16:0), palmidrol, Biologic Markers, Serum Marker, Surrogate, Endpoints, Function, Prp22, N-(2-Hydroxyethyl)palmitamide, palmidrolum, House Mice, Cognitive, Body, Surrogate Marker, Anhydrous Dextrose, Laboratory Mice, Protein Gene Products, Gene Proteins, Chromatography-Mass Spectrometry, cg8241, Insight., Bra, renin-binding protein, LC-MS, assay, Laboratory Mouse, Serums, Glc, Immune Markers"],"additional_accession":[]},"is_claimable":false,"name":"Deep and Quantitative Proteomic Profiling of Low Volume Mouse Serum Across the Lifespan","description":"We developed a nanoparticle-based LC-MS workflow optimized for low-volume mouse serum samples, enabling deep, quantitative, and reproducible proteome profiling. Applied to aging cohorts, the approach identified 3,992 protein groups across all samples and uncovered age-associated proteomic signatures in serum and linked to glucose and body composition. Complementary analysis using a 48-plex proximity extension assay (PEA) quantified an additional 39 cytokines not detected by MS, enhancing proteome coverage. This integrated platform supports robust biomarker discovery and systems-level insights in volume-limited preclinical studies.","dates":{"publication":"Wed Jul 02 01:05:00 BST 2025"},"accession":"MSV000098399","cross_references":{}}