{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v10/MSV000098529/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Michael Rape"],"instrument_platform":["Q Exactive GC Orbitrap"],"species":["Homo Sapiens (ncbitaxon:9606)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=37f7b71cf5604b039a5d5432ff2f581d"],"submitter_email":["mrape@berkeley.edu"],"submitter_affiliation":["University of California, Berkeley"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["33"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"pubmed_abstract":["The oxidative stress response is centered on the transcription factor NRF2 and protects cells from reactive oxygen species (ROS). While ROS inhibit the E3 ligase CUL3 <sup>KEAP1</sup> to stabilize NRF2 and elicit antioxidant gene expression, cells recovering from stress must rapidly reactivate CUL3 <sup>KEAP1</sup> to prevent reductive stress and oxeiptosis-dependent cell death. How cells restore efficient NRF2-degradation upon ROS clearance remains poorly understood. Here, we identify TRIP12, an E3 ligase dysregulated in Clark-Baraitser Syndrome and Parkinson's Disease, as a component of the oxidative stress response. TRIP12 is a ubiquitin chain elongation factor that cooperates with CUL3 <sup>KEAP1</sup> to ensure robust NRF2 degradation. In this manner, TRIP12 accelerates stress response silencing as ROS are being cleared, but limits NRF2 activation during stress. The need for dynamic control of NRF2-degradation therefore comes at the cost of diminished stress signaling, suggesting that TRIP12 inhibition could be used to treat degenerative pathologies characterized by ROS accumulation."],"pubmed_title":["Dynamic regulation of the oxidative stress response by the E3 ligase TRIP12."],"pubmed_authors":["Ingersoll Andrew J AJ, McCloud Devlon M DM, Hu Jenny Y JY, Rape Michael M"],"additional_accession":[]},"is_claimable":false,"name":"Dynamic regulation of the oxidative stress response by the E3 ligase TRIP12","description":"NRF2 FLAG IP to determine intracellular interactors","dates":{"publication":"Tue Jul 15 17:36:00 BST 2025"},"accession":"MSV000098529","cross_references":{"pubmed":["39651249"]}}