MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v10/MSV000098687/primaryOK200ProteomicsAudrey Odom JohnAndrew L. KauAgilent GC-qTOFC57bl/6 Micehttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=1969b5b47be34e20a653eb863a5c9300Children's Hospital of PhiladelphiaWashington University School of Medicine, St. Louisjohna3@chop.eduakau@wustl.eduMassIVE28MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetThe gut microbiota is crucial to health, yet implementation of microbiota-based therapeutics is limited by the lack of rapid diagnostics. We hypothesize that breath contains gut microbe-derived volatile organic compounds (VOCs) reflecting microbiota composition and metabolism. In healthy children, we found that breath VOC composition (or volatilome), assessed by gas chromatography-mass spectrometry, correlates with gut microbiome composition and function. By capturing exhaled breath from human-stool-colonized and monocolonized gnotobiotic mice, we profiled breath VOCs and discovered that murine breath is also significantly influenced by the gut microbiome. VOCs from cultured gut microbes were identified in vivo in monocolonized gnotobiotic colonized mice. As a proof of principle, we demonstrated that exhaled breath predicts the abundance of a disease-associated bacterium, Eubacterium siraeum, in children with asthma. Altogether, our studies identify microbe-derived VOCs in breath, show that gut bacterial metabolism directly contributes to mammalian breath VOC profiles, and inform the development of non-invasive microbiome diagnostics.The gut microbiota shapes the human and murine breath volatilome.Aging in perspective: the evolution of medical and social policy.Hernandez-Leyva Ariel J AJ, Berna Amalia Z AZ, Bui Maggie H MH, Liu Yang Y, Rosen Anne L AL, Lint Michael A MA, Whiteside Samantha A SA, Jaeger Natalia N, McDonough Ryan T RT, Joardar Nikhilesh N, Santiago-Borges Jesús J, Tomera Christopher P CP, Luo Wentai W, Odom John Audrey R AR, Kau Andrew L ALSomers A R ARfalseExamples of output air collected from a mice ventilator Examples of output air collected from a mice ventilator. mzML files used to generate Figure 6S C. Wed Jul 30 10:09:00 BST 2025MSV00009868741576933