{"database":"MassIVE","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v12/MSV000100665/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"submitter":["Aykut Uren"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=21a00fd9ba4f4690b94c7e07f99b0f83"],"submitter_email":["au26@georgetown.edu"],"sample_protocol":[""],"repository":["MassIVE"],"file_size":["10"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"omics_type":["Proteomics"],"instrument_platform":["Orbitrap Fusion Lumos","nanoACQUITY UPLC"],"species":["Homo Sapiens (ncbitaxon:9606)"],"submitter_affiliation":["Georgetown University Medical Center"],"additional_accession":["PXD073850"]},"is_claimable":false,"name":"ROME BioID2 raw proteomics data from ROME KO manuscript","description":"For proteomics, samples were heated for 10 min at 95 degrees. After cooling down to room temperature, 4 uL of 500 mM IAA in 5%SDS was added to a final concentration of 40 mM and incubated in the dark for 30 min. Undissolved matter was centrifuged for 8 min at 13,000 x g. The supernatant was saved and used for downstream processing using a S-Trap column (ProtiFi, LLC).  Proteins were digested with sequencing-grade Lys-C/trypsin (Promega) by incubation at 37 degrees overnight.  Peptides were analyzed with a nanoAcquity UPLC system coupled with Orbitrap Fusion Lumos mass spectrometer (ThermoFisher) as previously described.","dates":{"publication":"Fri Jan 30 10:01:00 GMT 2026"},"accession":"MSV000100665","cross_references":{}}