MassIVEapplication/xmlftp://massive-ftp.ucsd.edu/v12/MSV000100665/primary200OKAykut Urenhttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=21a00fd9ba4f4690b94c7e07f99b0f83au26@georgetown.eduMassIVE10MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetProteomicsOrbitrap Fusion LumosnanoACQUITY UPLCHomo Sapiens (ncbitaxon:9606)Georgetown University Medical CenterPXD073850falseROME BioID2 raw proteomics data from ROME KO manuscriptFor proteomics, samples were heated for 10 min at 95 degrees. After cooling down to room temperature, 4 uL of 500 mM IAA in 5%SDS was added to a final concentration of 40 mM and incubated in the dark for 30 min. Undissolved matter was centrifuged for 8 min at 13,000 x g. The supernatant was saved and used for downstream processing using a S-Trap column (ProtiFi, LLC). Proteins were digested with sequencing-grade Lys-C/trypsin (Promega) by incubation at 37 degrees overnight. Peptides were analyzed with a nanoAcquity UPLC system coupled with Orbitrap Fusion Lumos mass spectrometer (ThermoFisher) as previously described.Fri Jan 30 10:01:00 GMT 2026MSV000100665