LIPID MAPS are used to annotate the identified metabolites. The main databases include KEGG, HMDB and LIPID MAPS. These databases are used to annotate the identified metabolites in order to understand the functional properties and classification of different metabolites.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse phase","Liquid Chromatography MS - positive - reverse phase"],"chromatography_protocol":["Chromatographic column: Manufacturer: Thermo Fisher Scientific; Model: Hypersil GOLD C18; Dimensions: 100 mm length x 2.1 mm inner diameter; Particle Size: 1.9 µm; Catalog Number: 25002-102130.
Column temperature: 40 °C
Flow rate: 0.2 mL/min
Positive mode: mobile phase A: 0.1% formic acid; Mobile phase B: methanol
Negative mode: Mobile phase A: 5 mM ammonium acetate ammonium, pH 9.0; Mobile phase B: methanol
"],"publication":["Enhanced photosynthetic CO2 fixation in mutualistic Chlorella sp. and Mesorhizobium loti co-cultures."],"submitter_affiliation":["Institute of soil science, Chinese academy of sciences"],"submitter_name":["Dandan Yao"],"organism_part":["Whole Organism"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Putative metabolite identification was achieved using the HMDB database. For sample preparation, 100 mg of microalgae was ground with liquid nitrogen, resuspended in precooled 80% methanol and 0.1% formic acid, followed by centrifugation and dilution. UHPLC-MS/MS analysis was conducted using a Vanquish UHPLC system and an Orbitrap Q Exactive mass spectrometer, with a 17 min linear gradient elution on a Hypesil Gold column. Positive/negative polarity modes were employed, with specific solvent gradients, and mass spectrometer settings. Putative metabolite identification was achieved using the HMDB database. For sample preparation, 100 mg of microalgae was ground with liquid nitrogen, resuspended in precooled 80% methanol and 0.1% formic acid, followed by centrifugation and dilution. UHPLC-MS/MS analysis was conducted using a Vanquish UHPLC system and an Orbitrap Q Exactive mass spectrometer, with a 17 min linear gradient elution on a Hypesil Gold column. Positive/negative polarity modes were employed, with specific solvent gradients, and mass spectrometer settings.
"],"organism":["Chlorella"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS11422"],"author":["Yao Dandan. 4123300678. 327 Lawn. yaodandan@issas.ac.cn. 4123300678."],"data_transformation_protocol":["The.raw file was imported into CD3.1 database search software for processing, and the retention time, mass-charge ratio and other parameters of each metabolite were simply selected, and then the retention time deviation of 0.2 min and mass deviation of 5 ppm were set for peak alignment of different samples, so as to make the identification more accurate. Then set mass deviation of 5 ppm, signal strength deviation of 30%, signal-to-noise ratio of 3, minimum signal strength, plus and ion and other information for peak extraction.
The metabolites were identified by comparison with mzCloud, mzVault and MassList, both high-resolution secondary spectrograph databases. The specific principles are as follows: The molecular weight of the metabolite was determined according to the mass charge ratio (m/z) of the parent ion in the primary mass spectrometry, and the molecular formula was predicted by mass number deviation (ppm) and admixture ion, and then matched with the database. The database containing secondary spectra is matched with the fragment ion, collision energy and other information of each metabolite in the database according to the actual secondary spectra to achieve secondary identification of metabolites
"],"study_factor":["Enzyme concentration"],"submitter_email":["yaodandan@issas.ac.cn"],"sample_collection_protocol":["The microalgae were treated with varying concentrations of MlCA (10, 20 mg/L), referred to as low MlCA and high MlCA treatments, with a control group (CK) serving as the baseline. After the experiment, the samples were stored at 4 °C for metabolome determination
"],"omics_type":["Metabolomics"],"study_design":["Metabolomics","Carbonic Anhydrase 6","microalgae","carbon fixation"],"curator_keywords":["Metabolomics","Carbonic Anhydrase 6","microalgae","carbon fixation"],"mass_spectrometry_protocol":["Scanning range: 100-1500 m/z
The ESI source Settings are as follows: Spray Voltage: 3.5 kV; Sheath gas flow rate: 35 psi; Aux Gas flow rate: 10 L/min; Ion transfer tube temperature (Capillary Temp): 320 °C; Ion import RF level (S-lens RF level): 60; Aux gas heater temp: 350 °C; Polarity: positive, negative; MS/MS Secondary Scanning: data-dependent scans
"],"metabolite_name":["Skimmin","Corchorifatty acid F","Ascorbic acid","Sucrose","(-)-secoisolariciresinol","Leu-Pro","Corticosterone","Panthenol","Curculigoside","Palmitic acid","19(R)-Hydroxy-prostaglandin E2","Guan-fu base A","2-Hydroxyhippuric acid","Verbenalin","Pyrroloquinoline quinone","Praeruptorin B","Cytidine-5'-monophosphate","16-Hydroxyhexadecanoic acid","Hesperetin 5-O-glucoside","Germacrone","Uridine diphosphate glucose","Artesunate","Kynurenic acid","Deoxycholic Acid","Bergenin","gamma-Glutamylleucine","S-Sulfo-L-cysteine","Esculin","2-Isopropylmalic acid","LPC 18:1","8-Hydroxyguanosine","2'-Deoxyinosine","Geniposidic acid","alpha-Asarone","Ganoderic acid H","12-Hydroxydodecanoic acid","Arbutin","Arecoline","Capric acid","Procyanidin B2","5-Dehydroquinic acid","Hordatine B","N6-Succinyl Adenosine","Arachidic Acid","3-Dehydroshikimic acid","Mesaconic acid","Sclareolide","2-Oxoglutaric acid","Ingenol","Ademetionine","Atractylenolide III","Gingerglycolipid B","Oridonin","L-Threonic acid","trans-10-Heptadecenoic Acid","Patchouli alcohol","Pectolinarin","3-Hydroxyvaleric acid","14-Methylhexadecanoic Acid","Eugenyl acetate","L-Tryptophan","Glucose 1-phosphate","Valepotriate","3-Methyladipic acid","Uridine 5'-Diphospho-N-Acetylgalactosamine","5'-Adenylic acid","Ginkgolide C","Salicylic acid","Curcumol","Methyl Eugenol","Dodecanedioic acid","Thymidine 5'-monophosphate","UDP-galactose","Schisandrin C","1-Monopalmitin","Ginsenoside F1","dCMP","Citric acid","2-Methylglutaric acid","Psoralidin","Lauric Acid","tetranor-PGFM","Rosavin","P-Toluenesulfonic acid","Adenosine 5'-monophosphate","4-Pyridoxic acid","Tanshinone IIA","Glycerol 3-phosphate","Sugiol","(-)-chimonanthine","Guanosine","N-Acetyl-L-methionine","3-Hydroxydecanoic acid","Tauroursodeoxycholic acid","Thymidine","Prostaglandin F3alpha","4-tert-butylphenol","Aldosterone","Pentadecanoic Acid","Pseudolaric Acid B","2-Hydroxyphenylalanine","Phytocassane E","Isochlorogenic acid C","N-Tigloylglycine","Levodopa","Dihydroartemisinin","Myristic Acid","2-Hydroxycaproic acid","20-Hydroxyecdysone","Prostaglandin D2","Ursolic Acid","Methyl jasmonate","Apo-13-zeaxanthinone","(-)-Arctigenin","pteroside A","2-Hydroxy-2-methylbutyric acid","5-Sulfosalicylic acid","Bornyl acetate","Brassinolide","Uridine","Peimisine","Trillin","Thymidine 5'-diphosphate","3-Hydroxybenzoic acid","Vitamin B2","Hippuric acid","Trachelogenin","Elaidic acid","Thymol","10-Formyl-THF","Myristoleic Acid","Prostaglandin A1","Genipin","Isoeugenol","Schizandrol B","Vincamine","Azelaic acid","5-Methyluridine","Inosine diphosphate","Sinapyl Alcohol","2-Hydroxymyristic acid","Estrone sulfate","Methyl linoleate","Prostaglandin J2","Pogostone","D-ribose 5-phosphate","4-Isopropylbenzoic acid","Cyasterone","alpha-Zearalanol","2-Hydroxyestradiol","Kirenol","dAMP","3-Hydroxy-3-methylglutaric acid","N-Isovalerylglycine","10-Gingerol","Protectin D1","Estrone","Lumichrome"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced photosynthetic CO2 fixation in mutualistic Chlorella sp. and Mesorhizobium loti co-cultures","description":"It was further confirmed the stimulatory effect of M. loti-derived eCA on microalgae, highlighting key carbon metabolic pathways, including the Calvin cycle, TCA cycle and antioxidant defense response.
","dates":{"publication":"2025-01-20","submission":"2024-10-19"},"accession":"MTBLS11422","cross_references":{"HMDB":["HMDB0035612","HMDB0011599","HMDB0003331","HMDB0003282","HMDB0003099","HMDB0245964","HMDB0005049","HMDB0006031","HMDB0003871","HMDB0013656","HMDB0004680","HMDB0000101","HMDB0000905","HMDB0004326","HMDB0032142","HMDB0013817","HMDB0000292","HMDB0013651","HMDB0242634","HMDB0302754","HMDB0002641","HMDB0033766","HMDB0133970","HMDB0030254","HMDB0062121","HMDB0000754","HMDB0006116","HMDB0000732","HMDB0030027","HMDB0000459","HMDB0000479","HMDB0000355","HMDB0029306","HMDB0003464","HMDB0000500","HMDB0013894","HMDB0031617","HMDB0000017","HMDB0001173","HMDB0000763","HMDB0010217","HMDB0031624","HMDB0006203","HMDB0002658","HMDB0030801","HMDB0031085","HMDB0247418","HMDB0004669","HMDB0000034","HMDB0000050","HMDB0248142","HMDB0035796","HMDB0001147","HMDB0248395","HMDB0000031","HMDB0001043","HMDB0000052","HMDB0015643","HMDB0036656","HMDB0036562","HMDB0034054","HMDB0031048","HMDB0248849","HMDB0249055","HMDB0249168","HMDB0249187","HMDB0001008","HMDB0003134","HMDB0000030","HMDB0249428","HMDB0035710","HMDB0304284","HMDB0003154","HMDB0247207","HMDB0002121","HMDB0035824","HMDB0031867","HMDB0003066","HMDB0000097","HMDB0003441","HMDB0250266","HMDB0250270","HMDB0006709","HMDB0015466","HMDB0250407","HMDB0000063","HMDB0002802","HMDB0035220","HMDB0250582","HMDB0002214","HMDB0000078","HMDB0000089","HMDB0000630","HMDB0000163","HMDB0013609","HMDB0001321","HMDB0001401","HMDB0000127","HMDB0304820","HMDB0003213","HMDB0003423","HMDB0251511","HMDB0250994","HMDB0000016","HMDB0003581","HMDB0035104","HMDB0031565","HMDB0012215","HMDB0002183","HMDB0000583","HMDB0011632","HMDB0001999","HMDB0251767","HMDB0244828","HMDB0000068","HMDB0251918","HMDB0000349","HMDB0000153","HMDB0004472","HMDB0252457","HMDB0036769","HMDB0001397","HMDB0035304","HMDB0005812","HMDB0033897","HMDB0001514","HMDB0028929","HMDB0000132","HMDB0242331","HMDB0012273","HMDB0029598","HMDB0031159","HMDB0000157","HMDB0000738","HMDB0029737","HMDB0000734","HMDB0003320","HMDB0060484","HMDB0000195","HMDB0037318","HMDB0032797","HMDB0000696","HMDB0000472","HMDB0000168","HMDB0000904","HMDB0000148","HMDB0000725","HMDB0000684","HMDB0031398","HMDB0001645","HMDB0000159","HMDB0000267","HMDB0000279","HMDB0000167","HMDB0000158","HMDB0000191","HMDB0000182","HMDB0002184","HMDB0014623","HMDB0254014","HMDB0011175","HMDB0034225","HMDB0034277","HMDB0000673","HMDB0012252","HMDB0303673","HMDB0012254","HMDB0001296","HMDB0254414","HMDB0002005","HMDB0031740","HMDB0254600","HMDB0061859","HMDB0254615","HMDB0034363","HMDB0254854","HMDB0014554","HMDB0034181","HMDB0001200","HMDB0033971","HMDB0000866","HMDB0032055","HMDB0000812","HMDB0000803","HMDB0255056","HMDB0002064","HMDB0000678","HMDB0241916","HMDB0000821","HMDB0240595","HMDB0062175","HMDB0002099","HMDB0002189","HMDB0001406","HMDB0006809","HMDB0001488","HMDB0003269","HMDB0029846","HMDB0034737","HMDB0001721","HMDB0035858","HMDB0002117","HMDB0000207","HMDB0002088","HMDB0015224","HMDB0256022","HMDB0001867","HMDB0003229","HMDB0002100","HMDB0000210","HMDB0003263","HMDB0256345","HMDB0013302","HMDB0256418","HMDB0256449","HMDB0000263","HMDB0029377","HMDB0302993","HMDB0256776","HMDB0003411","HMDB0253028","HMDB0002982","HMDB0013041","HMDB0030759","HMDB0000239","HMDB0014351","HMDB0000244","HMDB0257364","HMDB0257463","HMDB0030024","HMDB0251013","HMDB0258172","HMDB0258165","HMDB0000792","HMDB0302242","HMDB0258235","HMDB0001257","HMDB0034146","HMDB0013078","HMDB0032523","HMDB0042015","HMDB0011725","HMDB0004826","HMDB0035846","HMDB0032062","HMDB0014613","HMDB0015002","HMDB0258793","HMDB0000234","HMDB0014897","HMDB0247895","HMDB0251530","HMDB0003252","HMDB0259107","HMDB0012204","HMDB0032538","HMDB0034263","HMDB0259297","HMDB0253473","HMDB0000306","HMDB0000300","HMDB0000288","HMDB0000301","HMDB0000883","HMDB0000208","HMDB0036792","HMDB0304435","HMDB0000072","HMDB0001120","HMDB0011741","HMDB0304704","HMDB0002048","HMDB0029686","HMDB0036565","HMDB0000186","HMDB0013624","HMDB0000229","HMDB0030087","HMDB0030280","HMDB0013692","HMDB0000972","HMDB0005783","HMDB0002059","HMDB0031067","HMDB0006294","HMDB0001908","HMDB0000071","HMDB0001987","HMDB0001624","HMDB0000338","HMDB0000840","HMDB0002261","HMDB0006050","HMDB0000402","HMDB0000422","HMDB0242587","HMDB0304122","HMDB0002466","HMDB0010725","HMDB0000531","HMDB0000555","HMDB0035268","HMDB0032063","HMDB0011617","HMDB0012710","HMDB0000884","HMDB0002044","HMDB0001185","HMDB0000045","HMDB0000037","HMDB0302026","HMDB0002212","HMDB0029943","HMDB0030353","HMDB0240267","HMDB0000044","HMDB0248708","HMDB0000784","HMDB0249087","HMDB0303194","HMDB0034081","HMDB0000511","HMDB0000094","HMDB0035919","HMDB0001547","HMDB0250592","HMDB0038122","HMDB0250618","HMDB0000095","HMDB0001548","HMDB0000626","HMDB0242686","HMDB0000623","HMDB0000573","HMDB0030820","HMDB0000145","HMDB0001425","HMDB0034122","HMDB0035987","HMDB0035830","HMDB0034942","HMDB0252704","HMDB0041094","HMDB0036860","HMDB0039555","HMDB0001586","HMDB0000126","HMDB0252967","HMDB00001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