MetaboLights

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matrix was then matched with the public databases HMDM (www.hmdb.ca/) and Metlin (http://www.metlin.scripps.edu/), as well as majorbio library, to obtain the metabolite information. After searching the library, the matrix data were uploaded to the cloud platform (http://www.cloud.majorbio.com) for data analysis, and the final analytical data matrix was obtained by 80% removal of missing values, sum normalisation, and Log10 logarithmic processing.MetaboLightsPublicLiquid Chromatography MS - negative - reverse phaseLiquid Chromatography MS - positive - reverse phaseThe 2 μL sample was separated on a HSS T3 column and then entered into the mass spectrometry detection. Mobile phase A was 95% water + 5% acetonitrile and mobile phase B was 47.5& acetonitrile + 47.5% isopropanol + 5% water, both containing 0.1% formic acid. The flow rate was 0.40 mL/min, the column temperature was 40 ℃, and the separation gradient gradient was set as follows: 0-1 min, 0-5% B mobile phase; 1-2 min, 5-25% B mobile phase; 2-9 min, 25-100% B mobile phase; 9-13 min, 100% B mobile phase; 13-13.1 min, 100-0% B mobile phase; 13.1-16 min, 0% B mobile phase.Lactobacillus johnsonii-derived oxindole protects acute lung injury by reducing CXCL13.chongqing medical universityshixin tangLungmass spectrometry assay200 mg of lung samples were mixed with 400 μL of extraction solution for metabolite extraction. The sample solution was ground in a frozen tissue mill for 6 min and then extracted by low temperature ultrasonication for 30 min, and the sample was centrifuged at 4°C for 5 min at 13,000 g, then supernatant was taken for analysis, QC was used as the quality control sampleMus musculushttps://www.ebi.ac.uk/metabolights/MTBLS11438Shixin Tang. Chongqing Medical University. chongqing medical university, chongqing. 874617878@qq.com.Subsequently, the LC-MS raw data were imported into the metabolomics processing software Prognesisi QI (Waters Corporation, Milford, USA) for baseline filtering, peak identification, integration, retention time correction, and peak alignment, and finally obtained a data matrix containing retention time, mass-to-charge ratio, and peak intensity data.Lactobacillus johnsonii for 14 days874617878@qq.comLung tissues were collected after 14 days lactobacillus johnsonii treatment in acute lung injury mice Metabolomicsuntargeted metabolitesLactobacillus johnsoniiacute lung injuryuntargeted metabolitesLactobacillus johnsoniiacute lung injuryThe mass spectra were performed in positive and negative ion scanning modes with a mass scan range of m/z: 70-1050, positive ion voltage 3500 V, negative ion 2800 V, sheath gas 40 psi, auxiliary heated gas 100 psi, ion source heating temperature 400 °C, cyclic collision energies of 20-40-60 V. The resolution of the MS1 was 70,000, and the resolution of the MS2 was 17,500. resolution 17500.falseLactobacillus johnsonii-derived oxindole protects acute lung injury by reducing CXCL13Gut microbiota is altered in mice with acute lung injury (ALI). Supplementation with Lactobacillus johnsonii, which is deficient in it, improved ALI in mice, and we elucidated the underlying mechanisms of the improvement by metabolomics.2026-02-122024-11-10MTBLS11438